eLife

Latest preprint reviews

1. Peroxiredoxin 5 regulates osteogenic differentiation through interaction with hnRNPK during bone regeneration

   This article has 13 authors:

   1. Eunjin Cho
   2. Xiangguo Che
   3. Mary Jasmin Ang
   4. Seongmin Cheon
   5. Jinkyung Lee
   6. Kwang Soo Kim
   7. Chang Hoon Lee
   8. Sang-Yeop Lee
   9. Hee-Young Yang
   10. Changjong Moon
   11. Chungoo Park
   12. Je-Yong Choi
   13. Tae-Hoon Lee

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The research presented in this manuscript is focused on testing the role of peroxiredoxin (Prdx5) and heterogeneous nuclear ribonucleoprotein K (hnRNPK) in bone biology and osteoporosis. Using cell-base and animal models, as well as various experimental methodologies the authors demonstrated that Prdx5 is upregulated during osteogenesis but suppressed during osteoclastogenesis. This novel function Prdx5 was found to be associated with binding and regulation of hnRNPK which controls the expression of genes involved in osteoclastogenesis, such as osteocalcin (Ocn).

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

2. We couldn't get details of this article at this time:
   10.1101/2022.05.11.490678

3. Desiccation resistance differences in Drosophila species can be largely explained by variations in cuticular hydrocarbons

   This article has 7 authors:

   1. Zinan Wang
   2. Joseph P Receveur
   3. Jian Pu
   4. Haosu Cong
   5. Cole Richards
   6. Muxuan Liang
   7. Henry Chung

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper is an extensive analysis of the underlying basis of desiccation resistance in 50 Drosophila species from diverse habitats. The work suggests that the longer methyl-branched alkanes (mbCHC) of the cuticular hydrocarbons are critical for this resistance. The study, which informs on the evolution of desiccation resistance in flies, is well done, although the main hypothesis is currently only partially supported by coating experiments, which presently lack controls and would be greatly strengthened by "replacement" experiments to add mbCHCs to flies without CHCs. The work is of relevance to evolutionary biologists in general.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

4. Spatiotemporal properties of glutamate input support direction selectivity in the dendrites of retinal starburst amacrine cells

   This article has 10 authors:

   1. Prerna Srivastava
   2. Geoff de Rosenroll
   3. Akihiro Matsumoto
   4. Tracy Michaels
   5. Zachary Turple
   6. Varsha Jain
   7. Santhosh Sethuramanujam
   8. Benjamin L Murphy-Baum
   9. Keisuke Yonehara
   10. Gautam Bhagwan Awatramani

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This is an important paper that addresses a key mechanism that underlies the canonical computation of direction selectivity in the retina. By using fluorescence imaging of glutamate release from excitatory interneurons combined with a computational model of dendritic integration, the authors make a convincing case that the kinetics of glutamate release contributes to the direction-selectivity of individual neural processes in retinal neurons. This work will appeal to visual neuroscientists as well as cellular physiologists interested in dendritic computations.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

5. Interplay between PML NBs and HIRA for H3.3 dynamics following type I interferon stimulus

   This article has 10 authors:

   1. Constance Kleijwegt
   2. Florent Bressac
   3. Coline Seurre
   4. Wilhelm Bouchereau
   5. Camille Cohen
   6. Pascale Texier
   7. Thomas Simonet
   8. Laurent Schaeffer
   9. Patrick Lomonte
   10. Armelle Corpet

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In this manuscript, of interest to the fields of animal immunity and epigenetics, the authors investigate the crosstalk between PML Nuclear Bodies and HIRA, a member of the H3.3 histone chaperone complex, during inflammatory stress. This study raises interesting perspectives on how availability of HIRA could be regulated by PML Nuclear Bodies for histone deposition onto interferon-stimulated genes, which in turn, could be relevant for immune-response mediated gene regulation.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

6. Postsynaptic plasticity of cholinergic synapses underlies the induction and expression of appetitive memories in Drosophila

   This article has 14 authors:

   1. Carlotta Pribbenow
   2. Yi-chun Chen
   3. Michael-Marcel Heim
   4. Desiree Laber
   5. Silas Reubold
   6. Eric Reynolds
   7. Isabella Balles
   8. Raquel Suárez Grimalt
   9. Carolin Rauch
   10. Jörg Rösner
   11. Gregor Lichtner
   12. Sridhar R. Jagannathan
   13. Tania Fernández-d.V. Alquicira
   14. David Owald

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Synaptic plasticity can take place on the presynaptic and/or postsynaptic sites, and these two sites of plasticity are known to involve distinct mechanisms. Using a combined approach of physiology, Drosophila genetics, and behaviour, this study provides evidence that postsynaptic mechanisms underlie plasticity for olfactory learning. This complements the field knowledge that olfactory associative learning largely relies on the presynaptic mechanism in mushroom body neurons. The paper also emphasizes the similarities in learning and memory mechanisms between vertebrates and invertebrates.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

7. Regulated degradation of the inner nuclear membrane protein SUN2 maintains nuclear envelope architecture and function

   This article has 7 authors:

   1. Logesvaran Krshnan
   2. Wingyan Skyla Siu
   3. Michael Van de Weijer
   4. Daniel Hayward
   5. Elena Navarro Guerrero
   6. Ulrike Gruneberg
   7. Pedro Carvalho

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The paper provides fundamental information through the identification of an E3 ligase and kinase/phosphatase regulatory machinery that regulates the inner nuclear membrane protein SUN2 using a GFP-based assay. The data reveal a model involving extraction of ubiquitylation of SUN2 from the membrane by p97, which is an important contribution to the field. Although the biochemical evidence is solid on the GFP-tagged SUN2 protein, one question is the extent to which this pathway works on endogenous SUN2 and the extent to which this is a quality control mechanism for turnover of unassembled SUN2 or whether it acts on the fully assembled complex.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

8. A specific role for importin-5 and NASP in the import and nuclear hand-off of monomeric H3

   This article has 2 authors:

   1. Alonso Javier Pardal
   2. Andrew James Bowman

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This is a very fundamental study that challenges the paradigm that histones H3 and H4 are imported to the nucleus primarily as heterodimers. Instead this study provides compelling evidence that H3 and H4 are imported by importin 5 as monomers and dimerize on chaperones in the nucleus. The work is of relevance to colleagues studying nuclear import and epigenetic regulation.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

9. miRNA-27a is essential for bone remodeling by modulating p62-mediated osteoclast signaling

   This article has 8 authors:

   1. Shumin Wang
   2. Eri O Maruyama
   3. John Martinez
   4. Justin Lopes
   5. Trunee Hsu
   6. Wencheng Wu
   7. Wei Hsu
   8. Takamitsu Maruyama

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors show that MiR-27a affects osteoclast-mediated bone resorption but not osteoblast-mediated bone formation during skeletal remodeling. Through gene profiling and bioinformatics study authors also identify the specific target of miR-27a in the osteoclast gene. MiR-27a exerts its effects on osteoclast differentiation through modulation of P62. This paper is of interest to scientists within the field of bone biology. The manuscript data analysis and conclusion are clear and directly supporting the previous known findings.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

10. RPG acts as a central determinant for infectosome formation and cellular polarization during intracellular rhizobial infections

    This article has 9 authors:

    1. Beatrice Lace
    2. Chao Su
    3. Daniel Invernot Perez
    4. Marta Rodriguez-Franco
    5. Tatiana Vernié
    6. Morgane Batzenschlager
    7. Sabrina Egli
    8. Cheng-Wu Liu
    9. Thomas Ott

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      This work, which will be of interest to all who study plant-microbe interactions as well as plant cell biology, addresses a fundamental question in symbiosis, placing a classic nodulation defective mutant (rpg) into a plausible protein complex and establishing a hierarchy of "infectosome" assembly. Evidence includes convincing genetics and subcellular localization of components during establishment and maintenance of infection. The study also includes compelling new FLIM-based imaging techniques to distinguish signals from closely associated domains in plant cells.

    Reviewed by eLife