eLife

Latest preprint reviews

1. Label-free imaging of M1 and M2 macrophage phenotypes in the human dermis in vivo using two-photon excited FLIM

   This article has 8 authors:

   1. Marius Kröger
   2. Jörg Scheffel
   3. Evgeny A Shirshin
   4. Johannes Schleusener
   5. Martina C Meinke
   6. Jürgen Lademann
   7. Marcus Maurer
   8. Maxim E Darvin

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Kröger et al use 2-photon FLIM tomography to perform correlative imaging on in vitro, ex vivo and in vivo blood and skin cells to determine characteristic NADPH fluorescence lifetimes for M1 and M2 ends of macrophage spectrum. Interestingly, M1 and M2 macrophages, and all other tissue cells, had distinctive lifetime features leading to robust prediction of phenotypes, with ground trust defined by cytokine staining. They generate a decision tree that has ~90% accuracy in identifying M1 and M2 based on FLIM parameters and additional information. The ability to use two photon fluorescence lifetime tomography of NADPH fluorescence to identify macrophages and their inflammatory status in human tissues should open opportunities in experimental medicine and eventually medical diagnosis.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

2. pH-dependent 11° F1FO ATP synthase sub-steps reveal insight into the FO torque generating mechanism

   This article has 2 authors:

   1. Seiga Yanagisawa
   2. Wayne D Frasch

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper is of outstanding interest to the broad community of scientists interested in biological energy conversion in general and rotary ATPases in particular. The authors show that the 36{degree sign} power stroke in ATP synthesis is subdivided into two steps of 11{degree sign} and 25{degree sign} in the E. coli enzyme, which serves as a comparatively simple model of the fundamental and universally important process of ATP production in mitochondria and chloroplasts. By combining precise and sophisticated single-molecule studies with directed mutagenesis, this work provides the much-needed functional context for recent high-resolution cryo-EM structures of rotary ATPases.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

3. Widespread discrepancy in Nnt genotypes and genetic backgrounds complicates granzyme A and other knockout mouse studies

   This article has 8 authors:

   1. Daniel J Rawle
   2. Thuy T Le
   3. Troy Dumenil
   4. Cameron Bishop
   5. Kexin Yan
   6. Eri Nakayama
   7. Phillip I Bird
   8. Andreas Suhrbier

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper is of interest not only for immunologists studying the inflammation, but also for biomedical researchers studying various biological processes using C57BL/6 mice. The data in this paper indicate that genetic differences between C57BL/6 substrains can affect reproducibility and generalizability in a broad range of biological studies with mouse models reported to date.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

4. Genetic basis and dual adaptive role of floral pigmentation in sunflowers

   This article has 12 authors:

   1. Marco Todesco
   2. Natalia Bercovich
   3. Amy Kim
   4. Ivana Imerovski
   5. Gregory L Owens
   6. Óscar Dorado Ruiz
   7. Srinidhi V Holalu
   8. Lufiani L Madilao
   9. Mojtaba Jahani
   10. Jean-Sébastien Légaré
   11. Benjamin K Blackman
   12. Loren H Rieseberg

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This work identifies the primary genetic mechanism underlying UV-absorbance variation across the geographic range of sunflower (Helianthus annuus) and provides evidence that suggests that abiotic variables, rather than pollinators, may maintain this variation in H. annuus and perhaps Helianthus broadly. While claims about direct links to fitness in natural population remain untested, the authors synthesize an ambitious amount of work from an impressive breadth of methods (from transgenics to ecology) that will be of high interest to ecologists and evolutionary biologists.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

5. Respiration aligns perception with neural excitability

   This article has 4 authors:

   1. Daniel S Kluger
   2. Elio Balestrieri
   3. Niko A Busch
   4. Joachim Gross

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Kluger and colleagues investigated the influence of respiration on visual sensory perception in a near-threshold task and argue that the detected correlation between respiration phase and detection precision is liked to alpha power, which in turn is modulated by the phase of respiration. The main finding - that the moment-to-moment relationship between excitability and perception is coupled to the body's slower respiratory oscillation - poses a potentially important advance for advancing our understanding of how the brain-body system works as a whole.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

6. RNase III-mediated processing of a trans-acting bacterial sRNA and its cis-encoded antagonist

   This article has 2 authors:

   1. Sarah Lauren Svensson
   2. Cynthia Mira Sharma

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Campylobacter jejuni is serious food-borne pathogen and understanding how the various products necessary for pathogenesis are regulated is a key step in preventing its growth and/or treating disease. Here, Sharma and coworkers describe the complex pathway that leads to the maturation of two complementary regulatory RNAs and how one of the RNAs antagonizes the other to relieve repression of a virulence-related gene.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the reviewers.)

   Reviewed by eLife

7. Supracellular organization confers directionality and mechanical potency to migrating pairs of cardiopharyngeal progenitor cells

   This article has 5 authors:

   1. Yelena Y Bernadskaya
   2. Haicen Yue
   3. Calina Copos
   4. Lionel Christiaen
   5. Alex Mogilner

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In this study, the authors develop a model to understand the simplest form of collective migration represented by a pair of cardiopharyngeal progenitor cells. They propose that the collective migrates as a "supracell", with leader cells assuming a greater protrusive capability and trailer cells assuming greater retractive capability. They meticulously study the effects of leader-trailer and cell-matrix adhesivity, intracellular force distributions and noise on robustness of cell migration. They corroborate their simulation results with experiments. Overall, this study comprehensively demonstrates that migrating as a collective leads to more mechanically efficient and persistent migration than as a single motile cell.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

8. Emergence of a geometric pattern of cell fates from tissue-scale mechanics in the Drosophila eye

   This article has 3 authors:

   1. Kevin D Gallagher
   2. Madhav Mani
   3. Richard W Carthew

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper will have a high impact for all developmental and cell biologists who are interested in tissue patterning and organogenesis. It provides unexpected insights into the problem of regular spacing of sub-organ structures. The study is based on innovative live imaging technology with state of the art analysis tools.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript.)

   Reviewed by eLife

9. Sensing complementary temporal features of odor signals enhances navigation of diverse turbulent plumes

   This article has 3 authors:

   1. Viraaj Jayaram
   2. Nirag Kadakia
   3. Thierry Emonet

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript by Jayaram and colleagues uses computational modeling approaches to examine how temporal filtering of an odor signal contributes to navigation success in different odor environments. The manuscript advances the literature in considering how different algorithms may be optimal for different environments. Further evidence is required to more convincingly prove the intriguing trade-off between frequency and "intermittency" sensing described here.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

10. Mechanisms of distributed working memory in a large-scale network of macaque neocortex

    This article has 2 authors:

    1. Jorge F Mejías
    2. Xiao-Jing Wang

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      Mejias and Wang propose here the first large-scale model of the brain that actually performs a cognitive task. Previous models have focused on neural dynamics during the so-called "resting state", in which subjects are not performing any cognitive task - thus, resting. This study is therefore an important improvement in the field of large-scale modelling and will certainly become an influential reference for future modelling efforts.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

    Reviewed by eLife