eLife

Latest preprint reviews

1. A spatiotemporal reconstruction of the C. elegans pharyngeal cuticle reveals a structure rich in phase-separating proteins

   This article has 15 authors:

   1. Muntasir Kamal
   2. Levon Tokmakjian
   3. Jessica Knox
   4. Peter Mastrangelo
   5. Jingxiu Ji
   6. Hao Cai
   7. Jakub W Wojciechowski
   8. Michael P Hughes
   9. Kristóf Takács
   10. Xiaoquan Chu
   11. Jianfeng Pei
   12. Vince Grolmusz
   13. Malgorzata Kotulska
   14. Julie Deborah Forman-Kay
   15. Peter J Roy

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Cuticles are specialized extracellular matrices that cover the bodies of ecdysozoans, which make up 85% of all animals. How cuticles are formed is very poorly understood, in particular in light of the fact that cuticles are shed and regrown as animals grow. The authors present a comprehensively and carefully curated resource of the components of the pharyngeal cuticle of C. elegans and provide a spatio-temporal framework to understand cuticle assembly. In doing so, the authors propose a function for a large class of intrinsically disordered proteins (IDPs). The significance of this work is high because our understanding of both cuticle formation and of IDPs is poor.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

2. Recruitment of clathrin to intracellular membranes is sufficient for vesicle formation

   This article has 5 authors:

   1. Cansu Küey
   2. Méghane Sittewelle
   3. Gabrielle Larocque
   4. Miguel Hernández-González
   5. Stephen J Royle

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript reports a striking finding, which should be of interest to cell biologists and biophysicists. The authors use an innovative approach to recruit clathrin to mitochondrial membranes and observe the budding and fission of clathrin-coated vesicles. The study leads to a much clearer view of how the clathrin lattice functions in endocytosis.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

3. Filamentation modulates allosteric regulation of PRPS

   This article has 10 authors:

   1. Huan-Huan Hu
   2. Guang-Ming Lu
   3. Chia-Chun Chang
   4. Yilan Li
   5. Jiale Zhong
   6. Chen-Jun Guo
   7. Xian Zhou
   8. Boqi Yin
   9. Tianyi Zhang
   10. Ji-Long Liu

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors investigated the structure of phosphoribosyl pyrophosphate (PRPP) synthase (PRPPS) from Escherichia coli, a highly conserved enzyme from bacteria to mammals that catalyzes the synthesis of a key common compound for several metabolic pathways. Combining structural data with mutagenesis and activity assays, they demonstrate that the enzyme is regulated differently by allosteric effectors when assembled into one filament form or the other. The strength of the manuscript is the high-quality cryo-EM data, which allows the reconstruction of two different filament forms bound to different ligands.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

4. A connectomics-based taxonomy of mammals

   This article has 7 authors:

   1. Laura E Suarez
   2. Yossi Yovel
   3. Martijn P van den Heuvel
   4. Olaf Sporns
   5. Yaniv Assaf
   6. Guillaume Lajoie
   7. Bratislav Misic

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper uses an impressively rich dataset (obtained and curated by the authors) to compare the structural brain connectomes of many animals spanning 6 taxonomic orders. The approach is innovative and relies on graph theoretical measures to describe the connectivity, which means it can be done without the need to spatially/functionally match the brains. The authors find that there is more variability between than within order. They attribute this effect to changes in local connectivity features, whereas global patterns are preserved. The approach can potentially be a useful way to study phylogeny and brain evolution.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

5. Automated hippocampal unfolding for morphometry and subfield segmentation with HippUnfold

   This article has 7 authors:

   1. Jordan DeKraker
   2. Roy AM Haast
   3. Mohamed D Yousif
   4. Bradley Karat
   5. Jonathan C Lau
   6. Stefan Köhler
   7. Ali R Khan

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper presents an exciting new automated package to investigate the hippocampal organization in new ways. As such, this package will be equally interesting for the fundamental basic and clinical neurosciences.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

6. Nuclear receptor corepressor 1 controls regulatory T cell subset differentiation and effector function

   This article has 24 authors:

   1. Valentina Stolz
   2. Rafael de Freitas e Silva
   3. Ramona Rica
   4. Ci Zhu
   5. Teresa Preglej
   6. Patricia Hamminger
   7. Daniela Hainberger
   8. Marlis Alteneder
   9. Lena Müller
   10. Monika Waldherr
   11. Darina Waltenberger
   12. Anastasiya Hladik
   13. Benedikt Agerer
   14. Michael Schuster
   15. Tobias Frey
   16. Thomas Krausgruber
   17. Sylvia Knapp
   18. Clarissa Campbell
   19. Klaus Schmetterer
   20. Michael Trauner
   21. Andreas Bergthaler
   22. Christoph Bock
   23. Nicole Boucheron
   24. Wilfried Ellmeier

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The study shows that the LXRbeta - NCOR1 axis restricts the terminal differentiation of Treg cells into effector Tregs. It also suggests that, in addition to an impact on effector Treg differentiation, loss of NCOR1 leads to impaired suppression function in Treg cells. The results may contribute to our understanding of Treg cell differentiation and function.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

7. Obligate sexual reproduction of a homothallic fungus closely related to the Cryptococcus pathogenic species complex

   This article has 12 authors:

   1. Andrew Ryan Passer
   2. Shelly Applen Clancey
   3. Terrance Shea
   4. Márcia David-Palma
   5. Anna Floyd Averette
   6. Teun Boekhout
   7. Betina M Porcel
   8. Minou Nowrousian
   9. Christina A Cuomo
   10. Sheng Sun
   11. Joseph Heitman
   12. Marco A Coelho

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     There are various ways in which self-fertility has arisen in the fungal kingdom. This study describes a novel form of self-fertility that evolved in a species closely related to the Cryptococcus species causing serious human lung disease, in which sexual development is achieved by self signaling of a cognate pheromone and pheromone receptor pair. Through a combination of high-quality genomic analysis and experimental gene expression and manipulation work, the study adds to our understanding of the evolution and flexibility of fungal breeding systems. This work will be of interest to colleagues studying fungi as well as mating systems in any eukaryote.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

8. Spatial modulation of individual behaviors enables an ordered structure of diverse phenotypes during bacterial group migration

   This article has 6 authors:

   1. Yang Bai
   2. Caiyun He
   3. Pan Chu
   4. Junjiajia Long
   5. Xuefei Li
   6. Xiongfei Fu

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors present a study on the cohesion maintenance of E.coli during collective migration in a self-generated gradient. They performed experiments and complemented the study with a predictive model and simulation to understand how bacteria with different phenotype are able to move as a cohesive group and how the individual bacterium defines its own position within the group. Particularly interesting aspects of the study are the use of titration of behavior with chemoreceptor abundance and the use of potential wells to model the attraction of bacteria to the center of their cohesive group. This approach will be of interest to physicists and biologists interested in collective motility and migration.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript.The reviewers remained anonymous to the authors.)

   Reviewed by eLife

9. Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasis

   This article has 7 authors:

   1. Virender Kumar Pal
   2. Ragini Agrawal
   3. Srabanti Rakshit
   4. Pooja Shekar
   5. Diwakar Tumkur Narasimha Murthy
   6. Annapurna Vyakarnam
   7. Amit Singh

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In the present study Pal and colleagues provide evidence that hydrogen sulfide (H2S) inhibits HIV replication and reactivation by a variety of mechanisms including inhibition of NF-kB and and recruitment of the epigenetic silencer, YY1, to the HIV promoter. They further report that H2S helps to maintaining mitochondrial bioenergetics and redox homeostasis and suggest that inclusion of an H2S donor in current ART regimens may help to achieve a functional HIV-1 cure.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

10. Homeostatic interferon-lambda response to bacterial microbiota stimulates preemptive antiviral defense within discrete pockets of intestinal epithelium

    This article has 14 authors:

    1. Jacob A Van Winkle
    2. Stefan T Peterson
    3. Elizabeth A Kennedy
    4. Michael J Wheadon
    5. Harshad Ingle
    6. Chandni Desai
    7. Rachel Rodgers
    8. David A Constant
    9. Austin P Wright
    10. Lena Li
    11. Maxim N Artyomov
    12. Sanghyun Lee
    13. Megan T Baldridge
    14. Timothy J Nice

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      The paper provides information about the relative importance of the type I and type III interferon-driven gene expression and anti-viral responses, particularly focused on the role of the Intestinal microbiota to maintain background levels of type III (interferon lambda) signaling. Type III-driven gene expression is highly discontinuous in the epithelial layer and mainly at the villous tips with consequent effects on the kinetics of rotavirus model infections.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript.The reviewers remained anonymous to the authors.)

    Reviewed by eLife