eLife

Latest preprint reviews

1. Quantitative prediction of variant effects on alternative splicing in MAPT using endogenous pre-messenger RNA structure probing

   This article has 8 authors:

   1. Jayashree Kumar
   2. Lela Lackey
   3. Justin M Waldern
   4. Abhishek Dey
   5. Anthony M Mustoe
   6. Kevin M Weeks
   7. David H Mathews
   8. Alain Laederach

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript will be of interest to biologists who study RNA structure-function relationships in a broad range of systems, splicing researchers, and RNA structure bioinformaticians. An integrative analysis of RNA structure probing, model-based RNA folding energetics, cryo-EM data, and protein binding sequence motifs serves as the basis for a comprehensive, accurate, and robust framework for predictive models of splicing dynamics in a well-studied system. The modeling is leveraged by in silico mutagenesis that reveals novel insights into the mechanisms and tradeoffs that underlie the impact of disease-associated mutations on alternative splicing.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

2. Cell-surface tethered promiscuous biotinylators enable comparative small-scale surface proteomic analysis of human extracellular vesicles and cells

   This article has 7 authors:

   1. Lisa L Kirkemo
   2. Susanna K Elledge
   3. Jiuling Yang
   4. James R Byrnes
   5. Jeff E Glasgow
   6. Robert Blelloch
   7. James A Wells

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This report describes a new technique to detect the surface proteome of normal and myc-transformed cells in relation to extracellular vesicles from the same cells. The data obtained from this comparison may be useful in evaluating cell surface and extracellular vesicle marker proteins that may be of diagnostic value. The article could possibly be more interesting if the actual proteomic results of control vs Myc and cells vs extracellular vesicles were more extensively exploited.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

   Reviewed by eLife

3. Overriding impaired FPR chemotaxis signaling in diabetic neutrophil stimulates infection control in murine diabetic wound

   This article has 15 authors:

   1. Ruchi Roy
   2. Janet Zayas
   3. Sunil K Singh
   4. Kaylee Delgado
   5. Stephen J Wood
   6. Mohamed F Mohamed
   7. Dulce M Frausto
   8. Yasmeen A Albalawi
   9. Thea P Price
   10. Ricardo Estupinian
   11. Eileena F Giurini
   12. Timothy M Kuzel
   13. Andrew Zloza
   14. Jochen Reiser
   15. Sasha H Shafikhani

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This work is of interest to scientists studying pathogenesis-associated neutrophil dysfunction. Roy et al. investigated impaired wound healing associated with infected diabetic wounds, a major clinical problem. The data reveal substantial alterations in the functional competence of diabetic or glucose-exposed neutrophils to react to chemotactic signals and provide potential therapeutic strategies to improve neutrophil fitness and improve wound healing. Conclusions are supported by the data but the study, in its current stage, needs further analysis.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

4. Hepatic MIR20B promotes nonalcoholic fatty liver disease by suppressing PPARA

   This article has 17 authors:

   1. Yo Han Lee
   2. Hyun-Jun Jang
   3. Sounkou Kim
   4. Sun Sil Choi
   5. Keon Woo Khim
   6. Hye-jin Eom
   7. Jimin Hyun
   8. Kyeong Jin Shin
   9. Young Chan Chae
   10. Hongtae Kim
   11. Jiyoung Park
   12. Neung Hwa Park
   13. Chang-Yun Woo
   14. Chung Hwan Hong
   15. Eun Hee Koh
   16. Dougu Nam
   17. Jang Hyun Choi

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The manuscript by Lee et al. provides mechanistic insight into the regulatory role of micro RNAs in modulating nuclear receptor expression and function. This is likely to have a high impact on the field as nuclear receptor regulation of metabolic disease is well established, however, the molecular mechanisms governing this process still remains unknown largely. Lee et al.'s manuscript provides a molecular target (miR-20b) that holds therapeutic potential in improving hepatic steatosis.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

5. Synaptic mechanisms of top-down control in the non-lemniscal inferior colliculus

   This article has 5 authors:

   1. Hannah M Oberle
   2. Alexander N Ford
   3. Deepak Dileepkumar
   4. Jordyn Czarny
   5. Pierre F Apostolides

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper will be of interest to neuroscientists who wish to understand how descending cortical projections interact in auditory midbrain neurons with their ascending inputs. The results have implications for other sensory systems and potentially other subcortical structures too. The data support the main conclusions of the manuscript, but additional control experiments and clarification of some parts are needed to strengthen the conclusions drawn and ensure that the findings of this interesting study can provide the basis for future modelling work.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. All reviewers agreed to share their names with the authors.)

   Reviewed by eLife

6. A phosphoinositide and RAB switch controls early macropinocytosis

   This article has 8 authors:

   1. Hélène Spangenberg
   2. Marte Sneeggen
   3. Maria Mateo Tortola
   4. Camila Valenzuela
   5. Yuen-Yan Chang
   6. Harald Stenmark
   7. Camilla Raiborg
   8. Kay Oliver Schink

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study is of interest to the broader audience of cell biologists, as it aims to dissect the hierarchy of protein recruitment and lipid conversion events that may provide a potential mechanism for the formation, maturation and fate of macropinosomes. The conclusions are based on the observation that pharmacological inhibition of the lipid kinase VPS34, which generates the signalling lipid PI3P on endosomes, prevents accumulation of Rab5 on macropinosomes, blocking their maturation and causing them to re-fuse with the plasma membrane.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

7. Intact Drosophila central nervous system cellular quantitation reveals sexual dimorphism

   This article has 9 authors:

   1. Wei Jiao
   2. Gard Spreemann
   3. Evelyne Ruchti
   4. Soumya Banerjee
   5. Samuel Vernon
   6. Ying Shi
   7. R Steven Stowers
   8. Kathryn Hess
   9. Brian D McCabe

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript describes a pipeline to assess the number of a certain cell type in the larval Drosophila brain. Their work provides automated neuronal segmentation and topographical analysis methods for the whole larval nervous system organization in flies, revealing the previously unexpected sexual dimorphism. This paper may be of interest to the large class of neuroscientists and specialists, from those who use larval Drosophila as their study model to others who are generally interested in connectomics and transcriptomics, among others.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

8. Spatial tuning of face part representations within face-selective areas revealed by high-field fMRI

   This article has 5 authors:

   1. Jiedong Zhang
   2. Yong Jiang
   3. Yunjie Song
   4. Peng Zhang
   5. Sheng He

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     How the brain is organized to represent various concepts has long been a central cognitive neuroscience research topic. Zhang and colleagues investigated the spatial distribution of feature tuning for different face-parts within face-selective regions of human visual cortex using ultra-high resolution 7.0 T fMRI. The findings complement non-human primate studies of face-selective patches and will be of interest to psychologists and system neuroscientists.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

9. Chlamydomonas ARMC2/PF27 is an obligate cargo adapter for intraflagellar transport of radial spokes

   This article has 7 authors:

   1. Karl F Lechtreck
   2. Yi Liu
   3. Jin Dai
   4. Rama A Alkhofash
   5. Jack Butler
   6. Lea Alford
   7. Pinfen Yang

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper is of broad interest to readers interested in motile cilia and cargo transport mediated by intraflagellar transport (IFT). It examines how radial spokes are trafficked into cilia by IFT, which represents a key process in the assembly of motile cilia. The authors demonstrate that an adaptor protein (ARMC2) is needed for association of radial spokes with the IFT machinery. They also find that the interaction of ARMC2/radial spokes with individual IFT trains (or particles) occurs in a stochastic manner rather than there being a specialized subset of trains specifically designated for a particular cargo. The results support the key claims in the paper.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

10. Getting out of a mammalian egg: the egg tooth and caruncle of the echidna

    This article has 8 authors:

    1. Jane C. Fenelon
    2. Abbie Bennetts
    3. Neal Anthwal
    4. Michael Pyne
    5. Stephen D. Johnston
    6. Alistair R. Evans
    7. Abigail S. Tucker
    8. Marilyn B. Renfree

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      This paper addresses the development, fate and homology of the egg tooth and caruncle of the short-beaked echidna (Tachyglossus aculeatus) and will be of broad interest to workers in the fields of vertebrate evolutionary/developmental biology, comparative anatomy and palaeontology. This manuscript features high quality histological and tomographic data from an unprecedented developmental series of pre- and post-hatching echidna stages. The study is well-organised and clearly reported, though additional image data would strengthen the authors' conclusions.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

    Reviewed by eLife