eLife

Latest preprint reviews

1. An amphipathic helix in Brl1 is required for nuclear pore complex biogenesis in S. cerevisiae

   This article has 13 authors:

   1. Annemarie Kralt
   2. Matthias Wojtynek
   3. Jonas S Fischer
   4. Arantxa Agote-Aran
   5. Roberta Mancini
   6. Elisa Dultz
   7. Elad Noor
   8. Federico Uliana
   9. Marianna Tatarek-Nossol
   10. Wolfram Antonin
   11. Evgeny Onischenko
   12. Ohad Medalia
   13. Karsten Weis

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This work advances our understanding of the nuclear pore complex (NPC) biogenesis pathway by providing much needed additional insight into the function of one of the few NPC assembly factors, Brl1. It thus addresses a long-standing and fundamental question relevant to individuals interested in nuclear transport, nuclear cell biology, and membrane-protein interactions more generally.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

2. Complex pattern of facial remapping in somatosensory cortex following congenital but not acquired hand loss

   This article has 8 authors:

   1. Victoria Root
   2. Dollyane Muret
   3. Maite Arribas
   4. Elena Amoruso
   5. John Thornton
   6. Aurelie Tarall-Jozwiak
   7. Irene Tracey
   8. Tamar R Makin

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This is a remarkably ambitious study that has been skilfully executed on a strong number of control participants, amputees, and one-handers. The complementarity of state-of-the-art uni- and multi-variate analyses are in the service of the research question, and the paper is clearly written. The main contribution of this paper, relative to previous studies, resides in the mapping of multiple face-part all at once in the three groups. The study suggests that the deprived hand cortical territory is not invaded by the facial cortical neighbor, the forehead, but instead by the lips that are more distant but functionally related to the hand as it can be used to compensate hand loss for manipulating objects.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

3. Gas6 drives Zika virus-induced neurological complications in humans and congenital syndrome in immunocompetent mice

   This article has 30 authors:

   1. Joao Luiz Silva-Filho
   2. Lilian Gomes de Oliveira
   3. Leticia Monteiro
   4. Pierina L. Parise
   5. Nagela G. Zanluqui
   6. Carolina M. Polonio
   7. Carla Longo de Freitas
   8. Daniel A. Toledo-Teixeira
   9. William M. Souza
   10. Najara Bittencourt
   11. Mariene R. Amorim
   12. Julia Forato
   13. Stéfanie Primon Muraro
   14. Gabriela Fabiano de Souza
   15. Matheus Cavalheiro Martini
   16. Karina Bispos-dos-Santos
   17. Carla C. Judice
   18. Maria Laura Costa
   19. Rodrigo N. Angerami
   20. André R. R. Freitas
   21. Mariangela R. Resende
   22. Márcia T. Garcia
   23. Maria Luiza Moretti
   24. The Zika-Unicamp Network
   25. Laurent Renia
   26. Lisa F. P. Ng
   27. Carla V. Rothlin
   28. Fabio TM Costa
   29. Jean Pierre Schatzmann Peron
   30. José Luiz Proença-Modena

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The pathophysiology of Zika virus infection remains an area of high research interest. In this study, the authors use diverse experimental models to examine the potential role of Gas6 in Zika virus infection and associated neurological sequelae.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

4. Intrinsic excitability mechanisms of neuronal ensemble formation

   This article has 4 authors:

   1. Tzitzitlini Alejandre-García
   2. Samuel Kim
   3. Jesús Pérez-Ortega
   4. Rafael Yuste

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript reveals the contribution of intrinsic excitability to the formation of cortical neuronal ensembles. By combining optogenetic and electrophysiological approaches in vitro, the authors provide new insight regarding the role that plasticity of membrane excitability (intrinsic plasticity) plays in synaptic plasticity and the formation of memories.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

5. Transient exposure of a buried phosphorylation site in an autoinhibited protein

   This article has 3 authors:

   1. Simone Orioli
   2. Carl G. Henning Hansen
   3. Kresten Lindorff-Larsen

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The manuscript describes a metadynamics simulation-based characterization of the binding and unbinding dynamics of a buried phosphorylated residue in the inhibitory module to the functional domain of Vav1, in an effort to shed lights on the autoinhibition mechanism. The work led to a conformation-selection scenario of the event. The authors discussed the inconsistency between the computational findings and the NMR data, in terms of the free energy differences between the bound and unbound states. Further justification is required for the somewhat ad hoc choice of three collective variables for the metadynamics simulations, including two that are highly correlated.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

6. Comparative transcriptome analysis of human and murine choroidal neovascularization identifies fibroblast growth factor inducible-14 as phylogenetically conserved mediator of neovascular age-related macular degeneration

   This article has 8 authors:

   1. Julian Wolf
   2. Anja Schlecht
   3. Dennis-Dominik Rosmus
   4. Stefaniya Boneva
   5. Hansjürgen Agostini
   6. Günther Schlunck
   7. Peter Wieghofer
   8. Clemens Lange

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors present here an analysis of the gene expression patterns of lesions as found in patients wet age-related macular degeneration, known as choroidal neovascularization. This gene expression analysis is compared to an experimental mouse model. Using this analysis, the authors have identified a candidate target, FN14, as a potential target for the treatment of choroidal neovascularization. Identifying an alternative treatment modality is important for the treatment of these lesions beyond the current standard of care.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

7. A neural mechanism for detecting object motion during self-motion

   This article has 3 authors:

   1. HyungGoo R Kim
   2. Dora E Angelaki
   3. Gregory C DeAngelis

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper will be of broad interest to readers in the field of motion perception. The authors use concurrent psychophysics and single unit recordings, along with modeling, to investigate how primate cortical area MT uses specific visual signals to make inferences that distinguish between visual motion induced by self-motion and the motion of other objects in the world. The experiments and stimuli are expertly designed and the analyses are careful.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

8. Directing Cholangiocyte Morphogenesis in Natural Biomaterial Scaffolds

   This article has 3 authors:

   1. Quinton Smith
   2. Christopher Chen
   3. Sangeeta Bhatia

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper is of interest for cell biologists, developmental biologists and tissue engineers. The authors identify a combination of natural extracellular matrix and growth factors that enables to grow cholangiocytes as branched three-dimensional ducts in culture. The work is physiologically relevant and represents an interesting step forward in the study of bile duct formation and disease, although the cultured ducts could be characterized more in depth.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

9. Subcellular Dynamic Immunopatterning of Cytosolic Protein Complexes on Microstructured Polymer Substrates

   This article has 5 authors:

   1. Roland Hager
   2. Ulrike Müller
   3. Nicole Ollinger
   4. Julian Weghuber
   5. Peter Lanzerstorfer

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript describes a method of evaluating the steady state levels and kinetics of protein-protein interactions at the plasma membrane of living cells. This approach builds on previous work in this area, and with stronger validation and demonstration of biological applications, it may be complementary to other biochemical or imaging-based approaches to address important questions related to mechanisms of cell signaling.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

10. Structural model of microtubule dynamics inhibition by kinesin-4 from the crystal structure of KLP-12 –tubulin complex

    This article has 14 authors:

    1. Shinya Taguchi
    2. Juri Nakano
    3. Tsuyoshi Imasaki
    4. Tomoki Kita
    5. Yumiko Saijo-Hamano
    6. Naoki Sakai
    7. Hideki Shigematsu
    8. Hiromichi Okuma
    9. Takahiro Shimizu
    10. Eriko Nitta
    11. Satoshi Kikkawa
    12. Satoshi Mizobuchi
    13. Shinsuke Niwa
    14. Ryo Nitta

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      Here, Taguchi et al. study a member of the kinesin-4 family of motors, which is important in controlling microtubule length during normal development and maintenance. The authors aim to determine how a member of the kinesin-4 family is able to stabilize the tips of microtubules to suppress both their growth and shrinkage. This paper provides compelling data on KLP-12 by combining in vivo C. elegans work with in vitro single-molecule analysis and structural studies of the motor domain. The structure shows that KLP-12 bends tubulin heterodimers to a level that lies in between the extremes of bending by KIF5B (lattice stabilizer) and KIF2C (lattice destabilizer). This study will be of interest to those in the fields of neuronal development and cytoskeletal dynamics.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

    Reviewed by eLife