eLife

Latest preprint reviews

1. Revised International Staging System (R-ISS) stage-dependent analysis uncovers oncogenes and potential immunotherapeutic targets in multiple myeloma (MM)

   This article has 15 authors:

   1. Ling Zhong
   2. Peng Hao
   3. Qian Zhang
   4. Tao Jiang
   5. Huan Li
   6. Jialing Xiao
   7. Chenglong Li
   8. Lan Luo
   9. Chunbao Xie
   10. Jiang Hu
   11. Liang Wang
   12. Yuping Liu
   13. Yi Shi
   14. Wei Zhang
   15. Bo Gong

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper is of potential interest to a broad audience across myeloma study and single cell technology, as it implies a major adjustment to our current understanding of pathogenesis and treatment of myeloma. Overall the data quality is good, although reasonable alternative explanations of the data can be identified.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

2. SRSF6 balances mitochondrial-driven innate immune outcomes through alternative splicing of BAX

   This article has 6 authors:

   1. Allison R Wagner
   2. Chi G Weindel
   3. Kelsi O West
   4. Haley M Scott
   5. Robert O Watson
   6. Kristin L Patrick

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper is of interest to people studying how differentially spliced genes regulate biological processes, and in particular, those interested in the intersection of cell death and immunity. This work offers new insight into how an alternatively spliced protein with a well-known function in cell death regulates the basal expression of genes involved in immunity and sensitizes cells to apoptotic cell death. Overall, the major conclusions are supported by the data but more investigation is needed to support the mechanism by which BAX splicing is inducing the phenotypes observed.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

3. Single-cell characterization of human GBM reveals regional differences in tumor-infiltrating leukocyte activation

   This article has 10 authors:

   1. Philip Schmassmann
   2. Julien Roux
   3. Steffen Dettling
   4. Sabrina Hogan
   5. Tala Shekarian
   6. Tomás A Martins
   7. Marie-Françoise Ritz
   8. Sylvia Herter
   9. Marina Bacac
   10. Gregor Hutter

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study is valuable and contains results that are supported by convincing evidence. In the future, the observations could be further strengthened by independent validation, and by looking at larger numbers of patients, as well as by determining whether patient heterogeneity is either contributing to or obscuring certain patterns. The work will be of interest to a broad audience in the oncology and immunology fields as it is on a cancer type that does not respond well to immune checkpoint therapeutics.

   Reviewed by eLife

4. Hormone replacement therapy for postmenopausal atherosclerosis is offset by late age iron deposition

   This article has 10 authors:

   1. Tianze Xu
   2. Jing Cai
   3. Lei Wang
   4. Li Xu
   5. Hongting Zhao
   6. Fudi Wang
   7. Esther G Meyron-Holtz
   8. Fanis Missirlis
   9. Tong Qiao
   10. Kuanyu Li

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     In this potentially important study, the authors attempt to explain why hormone replacement therapy with estrogen is not effective in preventing atherosclerosis in post-menopausal women by showing that iron accumulation prevents the hormone replacement therapy benefit through negative regulation of estrogen receptor expression via Mdm2-mediated proteolysis. The strength of evidence is currently incomplete as control groups are missing and there is a lack of clear-cut evidence that this effect is related to the estradiol therapy in addition to the accumulation of iron in the post-menopausal state. The general public as well as specialists might find this work to be of interest.

   Reviewed by eLife

5. Neutrophil-mediated fibroblast-tumor cell il-6/stat-3 signaling underlies the association between neutrophil-to-lymphocyte ratio dynamics and chemotherapy response in localized pancreatic cancer: A hybrid clinical-preclinical study

   This article has 12 authors:

   1. Iago de Castro Silva
   2. Anna Bianchi
   3. Nilesh U Deshpande
   4. Prateek Sharma
   5. Siddharth Mehra
   6. Vanessa Tonin Garrido
   7. Shannon Jacqueline Saigh
   8. Jonathan England
   9. Peter Joel Hosein
   10. Deukwoo Kwon
   11. Nipun B Merchant
   12. Jashodeep Datta

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Iago De Castro et al, constitute an exciting study conveying to readers that neutrophil-to-lymphocyte ratio dynamics predict pancreatic cancer pathologic response to neoadjuvant therapy. Specifically, the authors aim to determine the effect of gemcitabine/paclitaxel and anti-Ly6G treatment on stromal T cells and CAF populations. They conclude that neutrophil-to-lymphocyte ratios are associated with survival following this treatment and use animal models to show metastatic effects allied to it. The authors attempt to convey that microenvironmental neutrophils could play a causal role in pancreatic cancer chemoresistance. It was agreed that while the discoveries are very interesting, the public could benefit from the authors improving: the relevance to the human condition, providing a stronger link to fibroblastic cell functional transitions, broadening the discussion regarding previous/related published studies, and strengthening the anti-Ly6G specificity proof within the provided data.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

6. Early stage NSCLS patients’ prognostic prediction with multi-information using transformer and graph neural network model

   This article has 7 authors:

   1. Jie Lian
   2. Jiajun Deng
   3. Edward S Hui
   4. Mohamad Koohi-Moghadam
   5. Yunlang She
   6. Chang Chen
   7. Varut Vardhanabhuti

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This work presents a new model that leverages imaging and non-imaging data for the prediction of the survival of patients with early-stage NSCLC. The new model sought to demonstrate the roles of imaging and non-imaging features in determining high-risk nodes within the graph neural network, and the results have the potential of broad interest to clinicians within the field of cancer and have a high value towards clinical application.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewer remained anonymous to the authors.)

   Reviewed by eLife

7. Computational model of the full-length TSH receptor

   This article has 3 authors:

   1. Mihaly Mezei
   2. Rauf Latif
   3. Terry F Davies

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript presents the first molecular dynamics (MD) simulations of full-length membrane-bound Thyroid Stimulating Hormone Receptor (TSHR). The authors find that its linker region (LR) is disordered, contrasting previous models. While this is largely a solid study that would interest researchers working in computational modeling, thyroid hormone metabolism, and signaling, the rationale for the arbitrarily chosen starting model and unclear mechanistic relevance need to be clarified further.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

8. Novel fast pathogen diagnosis method for severe pneumonia patients in the intensive care unit: randomized clinical trial

   This article has 17 authors:

   1. Yan Wang
   2. Xiaohui Liang
   3. Yuqian Jiang
   4. Danjiang Dong
   5. Cong Zhang
   6. Tianqiang Song
   7. Ming Chen
   8. Yong You
   9. Han Liu
   10. Min Ge
   11. Haibin Dai
   12. Fengchan Xi
   13. Wanqing Zhou
   14. Jian-Qun Chen
   15. Qiang Wang
   16. Qihan Chen
   17. Wenkui Yu

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper is of broad interest to clinicians focused on improving sepsis outcomes. The method developed by the authors can identify 10 common pathogens with species-specificity in 4 hours, thus significantly reducing the turnaround time compared to conventional diagnostic methods. Using their method to identify sepsis-causing pathogens early to guide antibiotic treatment, the authors demonstrate high clinical sensitivity and specificity, and some clinical benefit in a real-world scenario.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

9. Kiaa1024L/Minar2 is essential for hearing by regulating cholesterol distribution in hair bundles

   This article has 6 authors:

   1. Ge Gao
   2. Shuyu Guo
   3. Quan Zhang
   4. Hefei Zhang
   5. Cuizhen Zhang
   6. Gang Peng

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Mutations in MINAR2 causes deafness in human and mice. Loss of function of Minar2 in mice causes a reduction of stereocilia and subsequent hair cell degeneration but the underlying mechanism is unclear. This zebrafish study demonstrated that the Minar2 protein interacts with cholesterol and is localized to the stereocilia of hair cells. The loss of Minar2 reduces cholesterol enrichment in the stereocilia with concomitant accumulation in lysosomes. Thus, this study provides the mechanistic insight of Minar2 and the first glimpse at the importance of cholesterol homeostasis in hair cell function.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

10. Hepatic lipid overload triggers biliary epithelial cell activation via E2Fs

    This article has 10 authors:

    1. Ece Yildiz
    2. Gaby El Alam
    3. Alessia Perino
    4. Antoine Jalil
    5. Pierre-Damien Denechaud
    6. Katharina Huber
    7. Lluis Fajas
    8. Johan Auwerx
    9. Giovanni Sorrentino
    10. Kristina Schoonjans

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      This important study reports that a high-fat diet induces biliary epithelial cell proliferation and suggests this may account for the so-called ductular reaction in advanced fatty liver disease. Convincing data support the finding that the transcription factor E2F1 is required for biliary epithelial cell proliferation in mice fed with a high-fat diet, and organoid models indicate that lipid abundance promotes glycolysis in an E2F-dependent manner. These findings are potentially of broad interest to the field of liver biology and disease.

    Reviewed by eLife