eLife

Latest preprint reviews

1. ACE2 is the critical in vivo receptor for SARS-CoV-2 in a novel COVID-19 mouse model with TNF- and IFNγ-driven immunopathology

   This article has 24 authors:

   1. Riem Gawish
   2. Philipp Starkl
   3. Lisabeth Pimenov
   4. Anastasiya Hladik
   5. Karin Lakovits
   6. Felicitas Oberndorfer
   7. Shane JF Cronin
   8. Anna Ohradanova-Repic
   9. Gerald Wirnsberger
   10. Benedikt Agerer
   11. Lukas Endler
   12. Tümay Capraz
   13. Jan W Perthold
   14. Domagoj Cikes
   15. Rubina Koglgruber
   16. Astrid Hagelkruys
   17. Nuria Montserrat
   18. Ali Mirazimi
   19. Louis Boon
   20. Hannes Stockinger
   21. Andreas Bergthaler
   22. Chris Oostenbrink
   23. Josef M Penninger
   24. Sylvia Knapp

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     To establish a mouse model for the SARS-CoV-2 infection, Gawish and colleagues performed serial passage of a human virus isolate in mice. They show that the mouse-adapted SARS-CoV-2 variant remains dependent on ACE2 for efficient infection and recapitulates some clinical characteristics of COVID-19. In addition, they demonstrate that inhalation of recombinant ACE2 protected mice from mouse COVID-19 suggesting this this model will be useful for the testing of antiviral agents.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife, ScreenIT

2. Identification of electroporation sites in the complex lipid organization of the plasma membrane

   This article has 6 authors:

   1. Lea Rems
   2. Xinru Tang
   3. Fangwei Zhao
   4. Sergio Pérez-Conesa
   5. Ilaria Testa
   6. Lucie Delemotte

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Using coarse-grained simulations, machine learning analysis and Bayesian inference modeling, the authors explored features that dictate the location and kinetics of electroporation in complex lipid membranes. The resulting understanding and modeling will lead to an effective multi-scale approach for predicting the kinetics of electroporation and guiding the design of experimental protocols for inducing electroporation in broad applications such as tumor treatment, gene therapy and vaccination against cancer.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

3. Cavefish cope with environmental hypoxia by developing more erythrocytes and overexpression of hypoxia-inducible genes

   This article has 2 authors:

   1. Corine M van der Weele
   2. William R Jeffery

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This article provides insight into how Astyanax mexicanus cavefish may have adapted to the hypoxic waters present in the cave environment. How the extreme environmental pressure of low oxygen has shaped cavefish evolution has been understudied compared to other pressures like absence of light or low nutrients. This is the first study to look for changes in early cavefish development that may provide hypoxia tolerance. The claims that cavefish have expanded erythrocyte development and increased hypoxia gene expression are strongly supported by the data. Demonstrating that these traits are adaptive and provide hypoxia tolerance requires further assessment of the current results and would be strengthened by additional experiments. Overall, this work is an important first step in understanding the evolution of hypoxia tolerance in A. mexicanus cavefish.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

4. Single-cell profiling reveals periventricular CD56bright NK cell accumulation in multiple sclerosis

   This article has 8 authors:

   1. Sabela Rodríguez-Lorenzo
   2. Lynn van Olst
   3. Carla Rodriguez-Mogeda
   4. Alwin Kamermans
   5. Susanne MA van der Pol
   6. Ernesto Rodríguez
   7. Gijs Kooij
   8. Helga E de Vries

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This is a well-written, well-illustrated and well-conducted study of the immune cell landscape of multiple sclerosis (MS) tissue, with a particular focus on the periventricular region (septum) and choroid plexus, using single cell mass cytometry (CyTOF). Overall the work is an impressive analysis of an understudied cell-type in MS, and represents an important resource. It will be important to follow up to establish how representative the findings are given the heterogeneity of the disease and the limited population studied here.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

5. Regulation of ERK2 activity by dynamic S-acylation

   This article has 4 authors:

   1. Saara-Anne Azizi
   2. Tian Qiu
   3. Noah E. Brookes
   4. Bryan C. Dickinson

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript describes the regulation of ERK1/2, two protein kinases that play important roles in cell signaling, by protein cysteine palmitoylation. The intriguing observations reported here could be of broad interest to colleagues in the field of cell signaling and protein post-translational modifications. Mechanistic understanding is, however, still limited and the work would benefit from additional experimental evidence.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

6. History-dependent physiological adaptation to lethal genetic modification under antibiotic exposure

   This article has 4 authors:

   1. Yuta Koganezawa
   2. Miki Umetani
   3. Moritoshi Sato
   4. Yuichi Wakamoto

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper presents results showing the temporal relationships between deletion of a resistance gene, introduction of antibiotic, and cell growth that are intriguing and novel. It will be of interest to researchers studying heterogeneity in antibiotic tolerance and the origins of drug resistance.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

7. Dorsal striatum coding for the timely execution of action sequences

   This article has 5 authors:

   1. Maria Cecilia Martinez
   2. Camila Lidia Zold
   3. Marcos Antonio Coletti
   4. Mario Gustavo Murer
   5. Mariano Andrés Belluscio

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript investigates an important topic related to the initiation signals of actions sequences detected in the dorsal striatum. The authors conduct an ambitious set of experiments to study how neural activity in the dorsal striatum relates to the ability to wait for a reward. The study nicely bridges research on striatum's roles in reward-seeking actions and in time processing. Interesting activity patterns are detected that suggest a relationship to the premature versus the timely release of actions. These observations are potentially interesting, in particular, the possible difference between adult and adolescent rats. The functional significance of these activity patterns remain to be examined.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

8. Integrative analysis of scRNA-seq and scATAC-seq revealed transit-amplifying thymic epithelial cells expressing autoimmune regulator

   This article has 23 authors:

   1. Takahisa Miyao
   2. Maki Miyauchi
   3. S Thomas Kelly
   4. Tommy W Terooatea
   5. Tatsuya Ishikawa
   6. Eugene Oh
   7. Sotaro Hirai
   8. Kenta Horie
   9. Yuki Takakura
   10. Houko Ohki
   11. Mio Hayama
   12. Yuya Maruyama
   13. Takao Seki
   14. Hiroto Ishii
   15. Haruka Yabukami
   16. Masaki Yoshida
   17. Azusa Inoue
   18. Asako Sakaue-Sawano
   19. Atsushi Miyawaki
   20. Masafumi Muratani
   21. Aki Minoda
   22. Nobuko Akiyama
   23. Taishin Akiyama

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The present work by Miyao and Miyauchi et al. provides new insights into the heterogeneity of mTEC. With single-cell approaches and the Fucci2a mouse model, they have found a proliferating mTEC sub-population that may be a precursor of mature mTECs expressing the Aire gene. The findings are potentially important for the field.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

9. CD47 cross-dressing by extracellular vesicles expressing CD47 inhibits phagocytosis without transmitting cell death signals

   This article has 11 authors:

   1. Yang Li
   2. Yan Wu
   3. Elena A Federzoni
   4. Xiaodan Wang
   5. Andre Dharmawan
   6. Xiaoyi Hu
   7. Hui Wang
   8. Robert J Hawley
   9. Sean Stevens
   10. Megan Sykes
   11. Yong-Guang Yang

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The manuscript shows that CD47, which provides inhibitory signals to myeloid cells via SIRP1alpha, is released in exosomal form by cells expressing it, and these exosomes can stick stably to other cells, resulting in CD47-mediated protection of these cells from phagocytosis by myeloid cells. The data are striking, and would be of interest to researchers working in both mechanistic and tranlational aspects of transplant-related immunoregulation.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

10. Molecular structures and conformations of protocadherin-15 and its complexes on stereocilia elucidated by cryo-electron tomography

    This article has 6 authors:

    1. Johannes Elferich
    2. Sarah Clark
    3. Jingpeng Ge
    4. April Goehring
    5. Aya Matsui
    6. Eric Gouaux

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      The manuscript by Elferich et al. describes challenging experiments to visualize components of the hair-cell mechanotransduction complex, especially PCDH15, in unfixed, fast-frozen stereocilia. The manuscript presents exquisite images of PCDH15 in its native state, confirming the predictions of earlier studies. While much of what is presented here was predicted from less-direct measurements, it is useful to confirm these with direct imaging of single molecules.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

    Reviewed by eLife