eLife

Latest preprint reviews

1. Disruption of the TCA cycle reveals an ATF4-dependent integration of redox and amino acid metabolism

   This article has 14 authors:

   1. Dylan Gerard Ryan
   2. Ming Yang
   3. Hiran A Prag
   4. Giovanny Rodriguez Blanco
   5. Efterpi Nikitopoulou
   6. Marc Segarra-Mondejar
   7. Christopher A Powell
   8. Tim Young
   9. Nils Burger
   10. Jan Lj Miljkovic
   11. Michal Minczuk
   12. Michael P Murphy
   13. Alex von Kriegsheim
   14. Christian Frezza

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors of this study investigate the consequences of acute or chronic disruption of parts of the TCA cycle, and how different interventions can drive different transcriptional responses. Specifically, the authors use both pharmacological and genetic methods to disrupt succinate dehydrogenase or fumarate hydratase, and characterize the effect of each on metabolism. They also find that disruption of these enzymes elicits a transcriptional response through ATF4. This work provides insight into how metabolism is affected by TCA cycle loss, and how how this affects metabolic stress signaling.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

2. Two functionally distinct Purkinje cell populations implement an internal model within a single olivo-cerebellar loop

   This article has 2 authors:

   1. Dora E. Angelaki
   2. Jean Laurens

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript features high quality experimental data with a detailed and clear analysis, combined with a neural network model to address the concept of differentiation in cerebellar functioning. This is an intensively debated topic currently and this work has an important, clear message to add to that debate. The data is very exciting, and the analyses and computational modeling very revealing and insightful. This stands on its own as a major contribution. The authors also raise an extremely interesting mechanistic interpretation of these data, which is tantalising but requires further support.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

3. Evolution of host-microbe cell adherence by receptor domain shuffling

   This article has 7 authors:

   1. EmilyClare P Baker
   2. Ryan Sayegh
   3. Kristin M Kohler
   4. Wyatt Borman
   5. Claire K Goodfellow
   6. Eden R Brush
   7. Matthew F Barber

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Baker et al. investigates the molecular evolution in primates of one protein family, the CEACAMs, that are a recurrent target of bacterial surface adhesions at epithelial surfaces. They show that multiple members of this gene family have experienced repeated episodes of positive selection in primates, especially in the N-terminal domains that are associated with protein binding and go on to evaluate the functional consequences of these evolutionary changes.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

4. Motor memories of object dynamics are categorically organized

   This article has 5 authors:

   1. Evan Cesanek
   2. Zhaoran Zhang
   3. James N Ingram
   4. Daniel M Wolpert
   5. J Randall Flanagan

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper is of interest to scientists within the field of motor learning. Converging evidence from several behavioural experiments support key claims of the paper. However, it is unclear to what degree the reported effects can be strongly linked to motor versus cognitive systems, and to what degree they novel demand revision of existing theoretical frameworks.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

5. Disintegration promotes protospacer integration by the Cas1-Cas2 complex

   This article has 4 authors:

   1. Chien-Hui Ma
   2. Kamyab Javanmardi
   3. Ilya J Finkelstein
   4. Makkuni Jayaram

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In this manuscript, an in vitro Cas1-Cas2 model system is used to study the reaction used to insert foreign DNA elements into a CRISPR array during the adaptive phase of immunity. The authors propose that hydrolysis of one end of the transposon DNA may be the primary mechanism for the insertion of very small DNA elements (which are difficult to bend tightly) that are found for the proto spacer sequences, and that cellular repair pathways are responsible for ligating the CRISPR array back together in vivo. The findings additionally suggest that water-mediated disintegration has an unappreciated role in the generation of CRISPR arrays as part of the bacterial immune response. These hypotheses are intriguing and of potential interest to those in the CRISPR field. However, it is unclear how this in vitro study, which does not monitor the full the reaction (directionality is lost due to the lack of a PAM sequence in the substrate and several required cellular factors are missing), relates to transposition as it occurs in vivo. Overall, this is an interesting study that challenges the current thinking in the field, but it does not present sufficient evidence to establish the physiological significance of the observed effects, thereby limiting its potential broader impact.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

6. High-intensity interval training remodels the proteome and acetylome of human skeletal muscle

   This article has 10 authors:

   1. Morten Hostrup
   2. Anders Krogh Lemminger
   3. Ben Stocks
   4. Alba Gonzalez-Franquesa
   5. Jeppe Kjærgaard Larsen
   6. Julia Prats Quesada
   7. Martin Thomassen
   8. Brian Tate Weinert
   9. Jens Bangsbo
   10. Atul Shahaji Deshmukh

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary

     This paper will be of interest to readers in the fields of exercise physiology, muscle biology and energy metabolism. The authors provide a proteomic resource where changes in the skeletal muscle proteome and acetyl-proteome have been assessed following the increasingly popular exercise intervention of high intensity interval training (HIIT). The adaptive responses reported provide new insight into the metabolic, contractile and transcriptional changes in muscle, and may represent an excellent resource for stimulating future focussed molecular studies in the field.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

7. Excitatory neurotransmission activates compartmentalized calcium transients in Müller glia without affecting lateral process motility

   This article has 3 authors:

   1. Joshua M Tworig
   2. Chandler J Coate
   3. Marla B Feller

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Tworig and colleagues use the mouse retina to explore the motility of Muller glial processes during development and during retinal waves that drive intracellular calcium signals in Muller glia. This is an important topic, because astrocytes in the brain have been suggested to move relative to synapses during neuronal activity. By performing careful and rigorous experiments, the authors find Muller glia processes move during development, but are not driven to move by neuronal activity. This is an important finding that will be of interest to diverse groups of readers.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

8. A phosphoswitch at acinus-serine437 controls autophagic responses to cadmium exposure and neurodegenerative stress

   This article has 4 authors:

   1. Nilay Nandi
   2. Zuhair Zaidi
   3. Charles Tracy
   4. Helmut Krämer

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Initial insights were provided by yeast genetic experiments into the mechanisms of starvation-induced autophagy. Since malfunctioning of this process is involved in numerous diseases, there is a need for further understanding the role and regulation of autophagy in different physiological settings. This work convincingly shows that a newly identified phosphatase controls basal levels of autophagy via regulation of phospho-acinus levels and reveals how cadmium intoxication triggers a neuroprotective autophagic response in the popular animal model Drosophila.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

   Reviewed by eLife

9. Bisphosphonate drugs have actions in the lung and inhibit the mevalonate pathway in alveolar macrophages

   This article has 12 authors:

   1. Marcia A Munoz
   2. Emma K Fletcher
   3. Oliver P Skinner
   4. Julie Jurczyluk
   5. Esther Kristianto
   6. Mark P Hodson
   7. Shuting Sun
   8. Frank H Ebetino
   9. David R Croucher
   10. Philip M Hansbro
   11. Jacqueline R Center
   12. Michael J Rogers

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The manuscript by Munoz et al describes the effect of the bisphosphonate zoledronic acid on tissue-resident macrophages. For this, the investigators used both a fluorescent bisphosphonate derivative and an unmodified zoledronic acid, in combination with macrophage populations isolated from different tissues. Based on the pattern of cytokines released by macrophages in the presence of zoledronic acid, the authors conclude that administration of bisphosphonates could, in addition to preventing bone loss, boost immune responses and lessen the consequences of respiratory infections.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

10. Multisensory-motor integration in olfactory navigation of silkmoth, Bombyx mori, using virtual reality system

    This article has 5 authors:

    1. Mayu Yamada
    2. Hirono Ohashi
    3. Koh Hosoda
    4. Daisuke Kurabayashi
    5. Shunsuke Shigaki

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      This paper uses a multi-model virtual reality system to assess which combinations of visual, wind, and olfactory information male silk moths rely on to find a female. The overall conclusion is that for the moths to search effectively, wind direction information is an important input. Vision, on the other hand, while it is used to control angular velocity, does not appear to be important for the moths to search effectively. This paper is of interest to neuroscientists and engineers interested in how multimodal sensory input controls navigational behavior. The experiments and modeling effort provide an advance in our understanding of how odor and wind information are combined in male silkmoths as they search for females.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

    Reviewed by eLife