eLife

Latest preprint reviews

1. Aspirin’s effect on kinetic parameters of cells contributes to its role in reducing incidence of advanced colorectal adenomas, shown by a multiscale computational study

   This article has 5 authors:

   1. Yifan Wang
   2. C Richard Boland
   3. Ajay Goel
   4. Dominik Wodarz
   5. Natalia L Komarova

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This work develops a multistage/component mathematical model to analyze advanced colorectal adenomas and the impact that aspirin therapy has on adenoma formation rates. This study will be interesting to the cancer evolution community and in particular those interested in colorectal cancer incidence. While the model is mainly focused on aspirin chemoprevention, the model could be adapted to test other putative preventative agents, and thus could have a broad impact.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer 3 agreed to share their names with the authors.)

   Reviewed by eLife

2. Lactoferricins impair the cytosolic membrane of Escherichia coli within a few seconds and accumulate inside the cell

   This article has 11 authors:

   1. Enrico F Semeraro
   2. Lisa Marx
   3. Johannes Mandl
   4. Ilse Letofsky-Papst
   5. Claudia Mayrhofer
   6. Moritz PK Frewein
   7. Haden L Scott
   8. Sylvain Prévost
   9. Helmut Bergler
   10. Karl Lohner
   11. Georg Pabst

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper presents groundbreaking data on the effects of antimicrobial peptides on bacterial cells, obtained by time resolved small angle X-ray and neutron scattering experiments coupled to stopped-flow mixing. Application of this approach to cells is highly innovative and provides ms time resolution, and information on multiple length scales (from conformational changes in the cell, to structural changes in the membranes). This is an important extension of the effort of the scientific community to study model membranes. The main result is that the peptides reach the cytosol in a few seconds, accumulating to high concentrations. The data analysis should be improved, and many conclusions are speculative, in particular on the mechanism of entry of the peptides.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

3. Both consumptive and non-consumptive effects of predators impact mosquito populations and have implications for disease transmission

   This article has 11 authors:

   1. Marie C Russell
   2. Catherine M Herzog
   3. Zachary Gajewski
   4. Chloe Ramsay
   5. Fadoua El Moustaid
   6. Michelle V Evans
   7. Trishna Desai
   8. Nicole L Gottdenker
   9. Sara L Hermann
   10. Alison G Power
   11. Andrew C McCall

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript will be of interest to community ecologists working on the impact of predators on prey populations, as well as disease ecologists interested in understanding the potential role of predators on vector traits. The authors uncovered trends in the research that support beneficial impacts of predators on mosquito traits, from the standpoint of vector control.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

4. Analysis of rod/cone gap junctions from the reconstruction of mouse photoreceptor terminals

   This article has 11 authors:

   1. Munenori Ishibashi
   2. Joyce Keung
   3. Catherine W Morgans
   4. Sue A Aicher
   5. James R Carroll
   6. Joshua H Singer
   7. Li Jia
   8. Wei Li
   9. Iris Fahrenfort
   10. Christophe P Ribelayga
   11. Stephen C Massey

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper uses a powerful combination of imaging techniques to provide a thorough view of the structure of the gap junction network connecting rod and cone photoreceptors in the mouse retina. The main conclusion - that rod-cone coupling is much more prevalent than rod-rod or cone-cone coupling - is well supported by the data although some results require qualification. The main concern in review centers around the importance of this result beyond the retina community.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

   Reviewed by eLife

5. Disruption of the TCA cycle reveals an ATF4-dependent integration of redox and amino acid metabolism

   This article has 14 authors:

   1. Dylan Gerard Ryan
   2. Ming Yang
   3. Hiran A Prag
   4. Giovanny Rodriguez Blanco
   5. Efterpi Nikitopoulou
   6. Marc Segarra-Mondejar
   7. Christopher A Powell
   8. Tim Young
   9. Nils Burger
   10. Jan Lj Miljkovic
   11. Michal Minczuk
   12. Michael P Murphy
   13. Alex von Kriegsheim
   14. Christian Frezza

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The authors of this study investigate the consequences of acute or chronic disruption of parts of the TCA cycle, and how different interventions can drive different transcriptional responses. Specifically, the authors use both pharmacological and genetic methods to disrupt succinate dehydrogenase or fumarate hydratase, and characterize the effect of each on metabolism. They also find that disruption of these enzymes elicits a transcriptional response through ATF4. This work provides insight into how metabolism is affected by TCA cycle loss, and how how this affects metabolic stress signaling.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

6. Two functionally distinct Purkinje cell populations implement an internal model within a single olivo-cerebellar loop

   This article has 2 authors:

   1. Dora E. Angelaki
   2. Jean Laurens

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript features high quality experimental data with a detailed and clear analysis, combined with a neural network model to address the concept of differentiation in cerebellar functioning. This is an intensively debated topic currently and this work has an important, clear message to add to that debate. The data is very exciting, and the analyses and computational modeling very revealing and insightful. This stands on its own as a major contribution. The authors also raise an extremely interesting mechanistic interpretation of these data, which is tantalising but requires further support.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

7. Evolution of host-microbe cell adherence by receptor domain shuffling

   This article has 7 authors:

   1. EmilyClare P Baker
   2. Ryan Sayegh
   3. Kristin M Kohler
   4. Wyatt Borman
   5. Claire K Goodfellow
   6. Eden R Brush
   7. Matthew F Barber

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Baker et al. investigates the molecular evolution in primates of one protein family, the CEACAMs, that are a recurrent target of bacterial surface adhesions at epithelial surfaces. They show that multiple members of this gene family have experienced repeated episodes of positive selection in primates, especially in the N-terminal domains that are associated with protein binding and go on to evaluate the functional consequences of these evolutionary changes.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

8. Motor memories of object dynamics are categorically organized

   This article has 5 authors:

   1. Evan Cesanek
   2. Zhaoran Zhang
   3. James N Ingram
   4. Daniel M Wolpert
   5. J Randall Flanagan

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper is of interest to scientists within the field of motor learning. Converging evidence from several behavioural experiments support key claims of the paper. However, it is unclear to what degree the reported effects can be strongly linked to motor versus cognitive systems, and to what degree they novel demand revision of existing theoretical frameworks.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

9. Disintegration promotes protospacer integration by the Cas1-Cas2 complex

   This article has 4 authors:

   1. Chien-Hui Ma
   2. Kamyab Javanmardi
   3. Ilya J Finkelstein
   4. Makkuni Jayaram

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In this manuscript, an in vitro Cas1-Cas2 model system is used to study the reaction used to insert foreign DNA elements into a CRISPR array during the adaptive phase of immunity. The authors propose that hydrolysis of one end of the transposon DNA may be the primary mechanism for the insertion of very small DNA elements (which are difficult to bend tightly) that are found for the proto spacer sequences, and that cellular repair pathways are responsible for ligating the CRISPR array back together in vivo. The findings additionally suggest that water-mediated disintegration has an unappreciated role in the generation of CRISPR arrays as part of the bacterial immune response. These hypotheses are intriguing and of potential interest to those in the CRISPR field. However, it is unclear how this in vitro study, which does not monitor the full the reaction (directionality is lost due to the lack of a PAM sequence in the substrate and several required cellular factors are missing), relates to transposition as it occurs in vivo. Overall, this is an interesting study that challenges the current thinking in the field, but it does not present sufficient evidence to establish the physiological significance of the observed effects, thereby limiting its potential broader impact.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

10. High-intensity interval training remodels the proteome and acetylome of human skeletal muscle

    This article has 10 authors:

    1. Morten Hostrup
    2. Anders Krogh Lemminger
    3. Ben Stocks
    4. Alba Gonzalez-Franquesa
    5. Jeppe Kjærgaard Larsen
    6. Julia Prats Quesada
    7. Martin Thomassen
    8. Brian Tate Weinert
    9. Jens Bangsbo
    10. Atul Shahaji Deshmukh

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary

      This paper will be of interest to readers in the fields of exercise physiology, muscle biology and energy metabolism. The authors provide a proteomic resource where changes in the skeletal muscle proteome and acetyl-proteome have been assessed following the increasingly popular exercise intervention of high intensity interval training (HIIT). The adaptive responses reported provide new insight into the metabolic, contractile and transcriptional changes in muscle, and may represent an excellent resource for stimulating future focussed molecular studies in the field.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

    Reviewed by eLife