eLife

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1. We couldn't get details of this article at this time:
   10.1101/2023.03.16.532898

2. A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genes

   This article has 8 authors:

   1. Nicole A Ellis
   2. Kevin S Myers
   3. Jessica Tung
   4. Anne Davidson Ward
   5. Kathryn Johnston
   6. Katherine E Bonnington
   7. Timothy J Donohue
   8. Matthias P Machner

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study uses CRISPRi to silence multiple effectors in the pathogen, Legionella pneumophila. It provides a technique that will allow researchers to address functional redundancy amongst effectors, a problem that has persisted even after decades of study. The methodology used is convincing, and further improvement (such as using multiple guides per gene) can lead to the identification of novel virulence factors.

   Reviewed by eLife

3. Multiple repeat regions within mouse DUX recruit chromatin regulators to facilitate an embryonic gene expression program

   This article has 4 authors:

   1. Christina M. Smith
   2. Edward J. Grow
   3. Sean C. Shadle
   4. Bradley R. Cairns

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This useful study provides a systematic mutational analysis to elucidate mechanisms involved in transcriptional activation by the murine DUX protein, DUX is a master transcription factor regulating mammalian early embryonic gene activation and its human homolog DUX4 is also involved in a muscular disease, fascioscapulohumeral dystrophy (FSHD). The data are solid and the interpretations of the findings are reasonable. The work will be of interest to colleagues studying early embryonic development or FSHD.

   Reviewed by eLife

4. Heterogeneous non-canonical nucleosomes predominate in yeast cells in situ

   This article has 6 authors:

   1. Zhi Yang Tan
   2. Shujun Cai
   3. Alex J Noble
   4. Jon K Chen
   5. Jian Shi
   6. Lu Gan

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important paper exploits new cryo-EM tomography tools to examine the state of chromatin in situ. The experimental work is meticulously performed, with a vast amount of data collected, with convincing interpretation of possible nucleosomal structures in yeast. The majority of these nucleosomes appear not to possess stable octameric configurations. Although it cannot be excluded that there could be an unknown bias in class detection, we agree that the authors have gone as far as is possible, given constraints in current technology and analysis, to support their model. Despite the provocative nature of this report, it is our hope that such work will spark debate, and further the development of exciting new tools that can interrogate the dynamic nature of the nucleosome, and its associated functions in situ.

   Reviewed by eLife

5. Inositol pyrophosphate dynamics reveals control of the yeast phosphate starvation program through 1,5-IP8 and the SPX domain of Pho81

   This article has 8 authors:

   1. Valentin Chabert
   2. Geun-Don Kim
   3. Danye Qiu
   4. Guizhen Liu
   5. Lydie Michaillat Mayer
   6. Muhammed Jamsheer K
   7. Henning J Jessen
   8. Andreas Mayer

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This fundamental study describes the mechanisms for regulation of the phosphate starvation response in baker's yeast, clarifies the interpretations of prior data, and suggests a unifying mechanism across eukaryotes. The study provides compelling data, based on biochemical analyses, protein localization by fluorescence, and genetic approaches that 1,5-InsP8 is the phosphate nutrient messenger in yeast.

   Reviewed by eLife

6. Genetic validation of PfFKBP35 as an antimalarial drug target

   This article has 13 authors:

   1. Basil T Thommen
   2. Jerzy M Dziekan
   3. Fiona Achcar
   4. Seth Tjia
   5. Armin Passecker
   6. Katarzyna Buczak
   7. Christin Gumpp
   8. Alexander Schmidt
   9. Matthias Rottmann
   10. Christof Grüring
   11. Matthias Marti
   12. Zbynek Bozdech
   13. Nicolas MB Brancucci

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     FKBP35 is the only FK506-binding protein present in the malaria-causing parasite Plasmodium falciparum, and has been considered a promising drug target due to its high affinity to the macrolide compound FK506, an immunosuppressant with antiplasmodial activity. This study demonstrates the essentiality of FKBP35 in parasite growth, based on compelling genetic evidence. The data also suggest that FK506 may exert its antimalarial activity through FKBP35-independent mechanisms that have not yet been characterised. This important study will be of interest to scientists working on the parasite biology and antimalarial drug development.

   Reviewed by eLife

7. Determinants of sugar-induced influx in the mammalian fructose transporter GLUT5

   This article has 7 authors:

   1. Sarah E McComas
   2. Tom Reichenbach
   3. Darko Mitrovic
   4. Claudia Alleva
   5. Marta Bonaccorsi
   6. Lucie Delemotte
   7. David Drew

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The current manuscript investigates the energy landscape of the mammalian sugar porter GLUT5 using enhanced molecular dynamics simulations and biochemical assays. The approach generates important insights into the mechanism of GLUT5 conformational change, and into mechanistic diversity among the GLUT sugar porters more generally. The overall strategy is solid, but without an additional error analysis, the computational components remain incomplete. These findings will be of interest to the transporter and membrane biology communities.

   Reviewed by eLife

8. Inferring causal connectivity from pairwise recordings and optogenetics

   This article has 5 authors:

   1. Mikkel Elle Lepperød
   2. Tristan Stöber
   3. Torkel Hafting
   4. Marianne Fyhn
   5. Konrad Paul Kording

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This useful study adapts methods from causal inference to develop analytical tools for determining the causal influence of single cells on downstream neurons. The simulation evidence is solid, suggesting that these causal methods produce better estimates under biologically-relevant confounds given enough data, although the practical application of the method and the biophysics it relies on is unclear. Nonetheless, this application of causal methods developed in econometrics and other fields could suggest new ways to think about largely observational datasets in neuroscience.

   Reviewed by eLife

9. Interplay between VSD, pore, and membrane lipids in electromechanical coupling in HCN channels

   This article has 7 authors:

   1. Ahmad Elbahnsi
   2. John Cowgill
   3. Verena Burtscher
   4. Linda Wedemann
   5. Luise Zeckey
   6. Baron Chanda
   7. Lucie Delemotte

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     Hyperpolarised-activated and Cyclic Nucleotide-gated (HCN) channels are the only mammalian channels to open under hyperpolarisation, being important for their roles in cardiac and neuronal cells. The authors of this study use atomistic simulations to enforce changing interaction distances that have been identified from a cryoEM structure and a homology model based on the hERG channel. The simulations suggest state-dependent interactions involving pore and voltage sensor helices, as well as with lipids, leading the authors to propose a domino-like mechanism of activation. These findings will be of considerable interest to the ion channel community.

   Reviewed by eLife

10. Local angiogenic interplay of Vegfc/d and Vegfa controls brain region-specific emergence of fenestrated capillaries

    This article has 10 authors:

    1. Sweta Parab
    2. Olivia A Card
    3. Qiyu Chen
    4. Michelle America
    5. Luke D Buck
    6. Rachael E Quick
    7. William F Horrigan
    8. Gil Levkowitz
    9. Benoit Vanhollebeke
    10. Ryota L Matsuoka

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      This is a comprehensive approach to understanding the genetic requirements of the VEGF family in the generation of brain capillary structure in zebrafish. The manuscript combines vascular brain/meningeal anatomy revealed with a variety of reporter lines, with mutants for Wnt/β-catenin signaling and angiogenic cues. In particular revealing genetic redundancy in VEGF receptor family in an essential function in vasculature development.

      This paper is significant because it provides compelling evidence that features methods, data, and analyses more rigorous than the current state-of-the-art in analysis of brain vasculature development. The data derived from the reporter lines are convincing, and the germline mutations allow for solid visualization and quantification of results.

    Reviewed by eLife