eLife

Latest preprint reviews

1. Single-cell RNA sequencing analysis of shrimp immune cells identifies macrophage-like phagocytes

   This article has 8 authors:

   1. Peng Yang
   2. Yaohui Chen
   3. Zhiqi Huang
   4. Huidan Xia
   5. Ling Cheng
   6. Hao Wu
   7. Yueling Zhang
   8. Fan Wang

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The shrimp market is growing globally, with 8.12 tons produced in 2020. The market size is thought to reach $55 billion by 2027. Most of the market comes from farms, the white shrimp, Penaeus vannamei, being one of the most commonly farmed species worldwide. The present study provides a single cell transcriptional atlas of the white shrimp, P. vannamei, immune cells in the hemolymph, known as hemocytes. White shrimp single cell RNA sequencing studies uncovered two macrophage-like populations, one of them with markers similar to mammalian macrophages. These findings redefine the current classification of shrimp immune cells which has been done using morphological approaches and via targeted qPCR studies but never using single cell transcriptomics.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

2. PLK4 drives centriole amplification and apical surface area expansion in multiciliated cells

   This article has 6 authors:

   1. Gina M LoMastro
   2. Chelsea G Drown
   3. Aubrey L Maryniak
   4. Cayla E Jewett
   5. Margaret A Strong
   6. Andrew Jon Holland

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     PLK4 is the master regulator of centriole biogenesis, but whether it is also key for centriole amplification during differentiation of multiciliated cells (MCCs) has been questioned based on PLK4 chemical inhibition. Here, using mouse models engineered to lack PLK4 or PLK4 activity, LoMastro et al provide very compelling evidence that PLK4 and its activity are essential for centriole amplification in MCCs. Moreover, they show that centriole amplification in MCCs drives expansion of their apical surface. The findings will be of interest to cell biologists and experts interested in multi-ciliogenesis-related pathologies.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

3. Characterization of full-length CNBP expanded alleles in myotonic dystrophy type 2 patients by Cas9-mediated enrichment and nanopore sequencing

   This article has 12 authors:

   1. Massimiliano Alfano
   2. Luca De Antoni
   3. Federica Centofanti
   4. Virginia Veronica Visconti
   5. Simone Maestri
   6. Chiara Degli Esposti
   7. Roberto Massa
   8. Maria Rosaria D'Apice
   9. Giuseppe Novelli
   10. Massimo Delledonne
   11. Annalisa Botta
   12. Marzia Rossato

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     To precisely diagnose DM2 caused by CCTG repetition in CNBP, the authors established a Cas9-mediated target enrichment system followed by Nanopore sequencing and analysis. The authors are fully aware of the limitations of the current diagnostic tests of DM2 and efficiently presented what novel findings have been revealed by the Cas9 nanopore sequencing. The findings of the current study suggest that Cas9 nanopore sequencing can be very useful for accurate genetic diagnosis of DM2 and understanding the genotype-phenotype correlation of this disease.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

4. The skeletal muscle circadian clock regulates titin splicing through RBM20

   This article has 13 authors:

   1. Lance A Riley
   2. Xiping Zhang
   3. Collin M Douglas
   4. Joseph M Mijares
   5. David W Hammers
   6. Christopher A Wolff
   7. Neil B Wood
   8. Hailey R Olafson
   9. Ping Du
   10. Siegfried Labeit
   11. Michael J Previs
   12. Eric T Wang
   13. Karyn A Esser

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript will be of broad interest to the field of muscle biology, muscle physiology, exercise physiology, metabolism and circadian rhythms. This manuscript identifies a new molecular pathway that connects circadian rhythms to muscle structure and function through titin isoform switching.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

5. A moonlighting function of a chitin polysaccharide monooxygenase, CWR-1, in Neurospora crassa allorecognition

   This article has 6 authors:

   1. Tyler C Detomasi
   2. Adriana M Rico-Ramírez
   3. Richard I Sayler
   4. A Pedro Gonçalves
   5. Michael A Marletta
   6. N Louise Glass

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study identifies an important role for a lytic polysaccharide monooxygenase in allorecognition in the filamentous fungus Neurospora crassa, which is independent of the catalytic activity of this remarkable class of proteins. The study's findings are strongly supported through an interdisciplinary approach, combining microscopy with genetics and biochemistry. The study will be of great interest to fungal biologists and microbiologists, as well as biochemists studying carbohydrate-active enzymes.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

6. Epac2 in midbrain dopamine neurons contributes to cocaine reinforcement via enhancement of dopamine release

   This article has 11 authors:

   1. Xiaojie Liu
   2. Casey R Vickstrom
   3. Hao Yu
   4. Shuai Liu
   5. Shana Terai Snarrenberg
   6. Vladislav Friedman
   7. Lianwei Mu
   8. Bixuan Chen
   9. Thomas J Kelly
   10. David A Baker
   11. Qing-song Liu

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript reports that Epac2, a downstream effector of cAMP, positively regulates cocaine reward by altering dopamine release properties in the striatum. These results provide insight into Epac2 as a potential presynaptic molecular target through which dopamine signaling and drug taking might be manipulated and is of interest to scientists studying dopamine transmission and substance use disorders.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

7. Convergence of two global regulators to coordinate expression of essential virulence determinants of Mycobacterium tuberculosis

   This article has 6 authors:

   1. Hina Khan
   2. Partha Paul
   3. Ritesh Rajesh Sevalkar
   4. Sangita Kachhap
   5. Balvinder Singh
   6. Dibyendu Sarkar

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper will be of broad interest to those working on the regulation of gene expression and mycobacteria as it deals with the collaboration of two important transcription regulators. A combination of experiments indicates how a complex of two regulators selectively turns on gene expression of a few genes in intracellular pathogen Mycobacterium tuberculosis.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

8. Early anteroposterior regionalisation of human neural crest is shaped by a pro-mesodermal factor

   This article has 14 authors:

   1. Antigoni Gogolou
   2. Celine Souilhol
   3. Ilaria Granata
   4. Filip J Wymeersch
   5. Ichcha Manipur
   6. Matthew Wind
   7. Thomas JR Frith
   8. Maria Guarini
   9. Alessandro Bertero
   10. Christoph Bock
   11. Florian Halbritter
   12. Minoru Takasato
   13. Mario R Guarracino
   14. Anestis Tsakiridis

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript addresses the important question of how cell types acquire regional identity during embryonic development. The authors study the role of TBXT in the establishment of posterior identity and show unexpected temporally restricted and cell-specific modes of acquisition of posterior identities in neural crest and spinal cord cells. They conclude that Wnt signaling influences posterior identity acquisition in neural crest cells whereas FGF is the main driver for spinal cord axial patterning.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

9. Interleukin-33 regulates the endoplasmic reticulum stress of human myometrium via an influx of calcium during initiation of labor

   This article has 6 authors:

   1. Li Chen
   2. Zhenzhen Song
   3. Xiaowan Cao
   4. Mingsong Fan
   5. Yan Zhou
   6. Guoying Zhang

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper addresses an interesting an important topic bearing on the initiation of labor at the end of pregnancy, invoking interleukin-33 in an alteration of Ca2+ homeostasis in uterine smooth muscle. The study implicated altered IL-33 expression in the third trimester of pregnancy in the endoplasmic reticular stress that might be involved in initiating labor.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

10. Distinct population and single-neuron selectivity for executive and episodic processing in human dorsal posterior cingulate

    This article has 10 authors:

    1. Lyndsey Aponik-Gremillion
    2. Yvonne Y Chen
    3. Eleonora Bartoli
    4. Seth R Koslov
    5. Hernan G Rey
    6. Kevin S Weiner
    7. Daniel Yoshor
    8. Benjamin Y Hayden
    9. Sameer A Sheth
    10. Brett L Foster

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      This manuscript provides new insights into one of the most enigmatic brain regions; the posterior cingulate cortex. Using electrophysiological recordings from dorsal and ventral PCC subregions, the authors provide evidence for a dorsal-executive and ventral-episodic functional subdivision. This paper will be of high interest to a broad range of neuroscientists.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

    Reviewed by eLife