eLife

Latest preprint reviews

1. The Plasmodium falciparum artemisinin resistance-associated protein Kelch 13 is required for formation of normal cytostomes

   This article has 6 authors:

   1. Madel V. Tutor
   2. Gerald J. Shami
   3. Ghizal Siddiqui
   4. Darren J. Creek
   5. Leann Tilley
   6. Stuart A. Ralph

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     Resistance of Plasmodium falciparum to artemisinin, which has become a threat to malaria control, has been linked to mutations in the parasite protein K13. This study provides important new insights into the function of K13 in the endocytosis of hemoglobin, a central process for the activation of artemisinin derivatives. Conditional protein mislocalization combined with high-resolution imaging provides convincing evidence that K13 is involved in the formation of cytostomes, the structures involved in the endocytosis of host cytosol. This study will be of interest to scientists working on parasite biology as well as antimalarial drug resistance.

   Reviewed by eLife

2. We couldn't get details of this article at this time:
   10.1101/2023.06.09.544290

3. Cervical cancer screening improvements with self-sampling during the COVID-19 pandemic

   This article has 3 authors:

   1. Miriam Elfström
   2. Penelope Grace Gray
   3. Joakim Dillner

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This paper is of interest to researchers and policy makers involved in cervical cancer prevention. The paper provides insight into how the Covid19 pandemic accelerated changes in organized cervical cancer screening. The claim that self-sampling led to a major improvement of test coverage seems somewhat exaggerated and alternative hypotheses to those provided by the authors on the population who chose self-sampling are possible. Nonetheless, this is a valuable piece of work given the scope of the intervention(s) and the precedent it sets i.e. a crisis can in fact accelerate positive changes in screening that have been academic possibilities rather than practical realities.

   Reviewed by eLife

4. An antagonism between Spinophilin and Syd-1 operates upstream of memory-promoting presynaptic long-term plasticity

   This article has 7 authors:

   1. Niraja Ramesh
   2. Marc Escher
   3. Oriane Turrel
   4. Janine Lützkendorf
   5. Tanja Matkovic
   6. Fan Liu
   7. Stephan J Sigrist

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This paper addresses the important question of presynaptic homeostasis and convincingly demonstrates antagonistic interactions between Spinophilin and Syd-1 in this process. It also provides a useful hypothesis for the downstream mechanisms.

   Reviewed by eLife

5. A scalable and tunable platform for functional interrogation of peptide hormones in fish

   This article has 3 authors:

   1. Eitan Moses
   2. Roman Franek
   3. Itamar Harel

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     Moises and Harel generate an important set of novel molecular tools in African turquoise killifish, an innovative model to study aging biology. The new solid tools described in this paper can boost this buddying model system for broad biotechnological applications. The authors showcase the efficacy of their tools in the context of peptide hormones involved in growth and gonad development. The killifish community will greatly benefit from these novel tools and the relevance of the developed methods will likely go beyond the killifish community.

   Reviewed by eLife

6. Diverse evolutionary pathways challenge the use of collateral sensitivity as a strategy to suppress resistance

   This article has 10 authors:

   1. Rebecca EK Mandt
   2. Madeline R Luth
   3. Mark A Tye
   4. Ralph Mazitschek
   5. Sabine Ottilie
   6. Elizabeth A Winzeler
   7. Maria Jose Lafuente-Monasterio
   8. Francisco Javier Gamo
   9. Dyann F Wirth
   10. Amanda K Lukens

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study addresses an important question in the field of antimicrobial chemotherapy: whether combinations of enzyme inhibitors that select for mutations that confer resistance to one inhibitor and at the same time increased sensitization to the other inhibitor can provide a path towards mitigating resistance risks. The authors here investigated one such combination of inhibitors of Plasmodium falciparum DHODH (dihydroorotate dehydrogenase), finding that despite "collateral sensitivity", it was still possible to select a mutation that mediated resistance to both inhibitors without any change in parasite fitness. Additional cross-susceptibility and structural modeling strengthen this study, which is performed to a high technical standard and presents a convincing body of data.

   Reviewed by eLife

7. Candida albicans exhibits heterogeneous and adaptive cytoprotective responses to antifungal compounds

   This article has 13 authors:

   1. Vanessa Dumeaux
   2. Samira Massahi
   3. Van Bettauer
   4. Austin Mottola
   5. Anna Dukovny
   6. Sanny Singh Khurdia
   7. Anna Carolina Borges Pereira Costa
   8. Raha Parvizi Omran
   9. Shawn Simpson
   10. Jinglin Lucy Xie
   11. Malcolm Whiteway
   12. Judith Berman
   13. Michael T Hallett

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The valuable study by Dumeaux et al examines the transcriptional response to antifungal treatment in the major opportunistic human fungal pathogen Candida albicans. Using solid methodology, including a novel droplet-based single cell transcriptomics platform, the authors report that fungal cells exhibit heterogeneity in their transcriptional response to antifungal drug treatment. The ability to study the trajectories of individual cells in a high-throughput manner provides a novel perspective on studying the emergence of drug tolerance and resistance in fungal pathogens.

   Reviewed by eLife

8. Resting-state fMRI signals contain spectral signatures of local hemodynamic response timing

   This article has 4 authors:

   1. Sydney M Bailes
   2. Daniel EP Gomez
   3. Beverly Setzer
   4. Laura D Lewis

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This manuscript addresses the important issue of hemodynamic response function (HRF) variability across brain areas and will be valuable to researchers who use fMRI and other types of functional imaging that rely on neurovascular coupling. Using simulations and experiments, the authors provide solid evidence that differences in the HRF can impact spectrum-based metrics such as ALFF and fALFF. A better understanding of the variability of the HRF is critical for the proper interpretation of activation onset times and of differences observed in clinical populations where both neural and vascular alterations can be expected.

   Reviewed by eLife

9. Rab12 is a regulator of LRRK2 and its activation by damaged lysosomes

   This article has 16 authors:

   1. Xiang Wang
   2. Vitaliy V Bondar
   3. Oliver B Davis
   4. Michael T Maloney
   5. Maayan Agam
   6. Marcus Y Chin
   7. Audrey Cheuk-Nga Ho
   8. Rajarshi Ghosh
   9. Dara E Leto
   10. David Joy
   11. Meredith EK Calvert
   12. Joseph W Lewcock
   13. Gilbert Di Paolo
   14. Robert G Thorne
   15. Zachary K Sweeney
   16. Anastasia G Henry

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This valuable study shows that Rab12 is required for LRRK2 activation. However, while some of the data are compelling, some claims, especially the ones related to LRRK2's membrane association are not supported. Addressing discrepancies between figures (pointed out by reviewers) and re-writing certain sections will greatly improve this manuscript.

   Reviewed by eLife

10. The Reissner fiber under tension in vivo shows dynamic interaction with ciliated cells contacting the cerebrospinal fluid

    This article has 8 authors:

    1. Celine Bellegarda
    2. Guillaume Zavard
    3. Lionel Moisan
    4. Françoise Brochard-Wyart
    5. Jean-François Joanny
    6. Ryan S Gray
    7. Yasmine Cantaut-Belarif
    8. Claire Wyart

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      This exceptional work substantially advances our understanding of the mechanics of the Reissner's fibre (RF) by performing in-vivo experiments that track and analyze the behavior of the RF when it is cut and the behavior of ciliated cells touching the RF when contact is interrupted. The data is valuable and the conclusions are compelling. The work will be of broad interest to many research communities including developmental neuroscience and cilia biology.

    Reviewed by eLife