eLife

Latest preprint reviews

1. A computational method for predicting the most likely evolutionary trajectories in the stepwise accumulation of resistance mutations

   This article has 5 authors:

   1. Ruth Charlotte Eccleston
   2. Emilia Manko
   3. Susana Campino
   4. Taane G Clark
   5. Nicholas Furnham

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This report is a useful demonstration of how to predict the mutational pathways to antimicrobial resistance (AMR) emergence, particularly in the enzyme DHFR (dihydrofolate reductase). The methodology is overall solid but some of the claims are only partially supported. The work will be of interest to microbiologists and evolutionary biologists interested in antimicrobial resistance and its population genetics dynamic.

   Reviewed by eLife

2. Determining growth rates from bright-field images of budding cells through identifying overlaps

   This article has 6 authors:

   1. Julian MJ Pietsch
   2. Alán F Muñoz
   3. Diane-Yayra A Adjavon
   4. Iseabail Farquhar
   5. Ivan BN Clark
   6. Peter S Swain

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     In this interesting manuscript, Pietsch et al. develop innovative machine learning approaches for automated analysis of budding yeast live-cell imaging data obtained with a dedicated microfluidic device that retains mother cells. Developing such tools is crucial to enable high-throughput image analysis. These methods will be useful for researchers studying these cells, and may also inspire similar approaches for other types of cells.

   Reviewed by eLife

3. An extracellular vesicle targeting ligand that binds to Arc proteins and facilitates Arc transport in vivo

   This article has 5 authors:

   1. Peter H Lee
   2. Michael Anaya
   3. Mark S Ladinsky
   4. Justin M Reitsma
   5. Kai Zinn

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     Extracellular vesicles (EVs) are emerging as important mediators of cell-to-cell signaling. Lee et al show convincingly that Stranded at second (Sas), a Drosophila cell surface protein, is trafficked by and localized to Drosophila EVs. However, the data supporting interaction with dArc and whether Sas facilitates the intercellular transfer of dArc protein or mRNA is incomplete. Moreover, almost all experiments rely on gain-of-function and over-expression of Sas, thus the relevance to normal physiological signaling is unclear.

   Reviewed by eLife

4. Single-cell profiling coupled with lineage analysis reveals vagal and sacral neural crest contributions to the developing enteric nervous system

   This article has 4 authors:

   1. Jessica Jacobs-Li
   2. Weiyi Tang
   3. Can Li
   4. Marianne E Bronner

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This is an elegant study combining virally-delivered lineage-tracing with single cell RNA-sequencing of the developing chicken enteric nervous system, showing potentially differential contribution of cell identities from the sacral and vagal neural crest. Addressing this important issue is pivotal to understanding basic enteric nervous system development as well as to devise therapeutic approaches to enteric neuropathies. The study is therefore generally interesting and in particular to researchers in the fields of enteric neuroscience and peripheral nervous system development. Lack of a basic classification scheme of neuronal cell types in the chicken, limited computational and functional analysis on a relatively immature stage and makes the conclusions of this work preliminary in its current state.

   Reviewed by eLife

5. A single-cell atlas depicting the cellular and molecular features in human anterior cruciate ligamental degeneration: A single cell combined spatial transcriptomics study

   This article has 7 authors:

   1. Runze Yang
   2. Tianhao Xu
   3. Lei Zhang
   4. Minghao Ge
   5. Liwei Yan
   6. Jian Li
   7. Weili Fu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The creation of a single-cell atlas of normal and degenerative human anterior cruciate ligament (ACL) tissues using a single-cell RNA sequencing method is an important approach to understanding the pathological mechanisms of ACL degeneration. The data of this study showed the existence of fibroblasts, endothelial cells, pericytes, and immune cells in healthy ACL, and their ratios altered in the degenerative ACL, mainly exhibited as an increase in fibroblasts and immune cells. The data analysis suggests that alterations of spatial transcriptome and changes in gene expression and signaling pathways may contribute to ACL degeneration.

   Reviewed by eLife

6. Histone deacetylase 6 inhibition promotes microtubule acetylation and facilitates autophagosome–lysosome fusion in dystrophin‐deficient mdx mice

   This article has 5 authors:

   1. Akanksha Agrawal
   2. Erin L. Clayton
   3. Courtney L. Cavazos
   4. Benjamin A. Clayton
   5. George G. Rodney

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study pinpoints nitrite oxide synthase 2 activity and decreased microtubule acetylation as distinct regulators of altered autophagic flux that may contribute to pathogenesis in a mouse model of Duchenne muscular dystrophy. While most of the evidence to support these claims is convincing, the claim that autophagy is improved with increased microtubule acetylation is incompletely supported. This work may be of broad interest to muscle biologists and has translational potential for the treatment of Duchenne muscular dystrophy.

   Reviewed by eLife

7. The impact of COVID-19 on cancer screening and treatment in older adults: The Multiethnic Cohort Study

   This article has 6 authors:

   1. Victoria P Mak
   2. Kami White
   3. Lynne R Wilkens
   4. Iona Cheng
   5. Christopher A Haiman
   6. Loic Le Marchand

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The authors used the Multiethnic Cohort (MEC) study to study how COVID-19 impacted access to cancer screenings and treatment. This study's important findings served to identify key factors associated with cancer-related screening and healthcare-seeking during the pandemic. This investigation provides solid evidence to inform future policies, particularly in older and vulnerable populations.

   Reviewed by eLife

8. Associations of four biological age markers with child development: A multi-omic analysis in the European HELIX cohort

   This article has 20 authors:

   1. Oliver Robinson
   2. ChungHo E Lau
   3. Sungyeon Joo
   4. Sandra Andrusaityte
   5. Eva Borras
   6. Paula de Prado-Bert
   7. Lida Chatzi
   8. Hector C Keun
   9. Regina Grazuleviciene
   10. Kristine B Gutzkow
   11. Lea Maitre
   12. Dries S Martens
   13. Eduard Sabido
   14. Valérie Siroux
   15. Jose Urquiza
   16. Marina Vafeiadi
   17. John Wright
   18. Tim S Nawrot
   19. Mariona Bustamante
   20. Martine Vrijheid

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This is an important study that examined multiple biological age measures in children, which has been lacking in literature. The findings of this study provided convincing evidence to interpret and understand the aging and developmental processes in children.

   Reviewed by eLife

9. Comparative single-cell profiling reveals distinct cardiac resident macrophages essential for zebrafish heart regeneration

   This article has 9 authors:

   1. Ke-Hsuan Wei
   2. I-Ting Lin
   3. Kaushik Chowdhury
   4. Khai Lone Lim
   5. Kuan-Ting Liu
   6. Tai-Ming Ko
   7. Yao-Ming Chang
   8. Kai-Chien Yang
   9. Shih-Lei (Ben) Lai

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The authors analyze changes in the gene expression of different immune cells during heart regeneration using single-cell RNA-sequencing and assess changes upon drug treatment that depletes macrophages. They find that drug treatment affects the gene expression profiles of different and abundance of immune cells. The work provides a wealth of gene expression data and a nice analysis supporting immune cell interactions during heart regeneration, so will be a useful resource.

   Reviewed by eLife

10. Tracing the development and lifespan change of population-level structural asymmetry in the cerebral cortex

    This article has 14 authors:

    1. James M Roe
    2. Didac Vidal-Pineiro
    3. Inge K Amlien
    4. Mengyu Pan
    5. Markus H Sneve
    6. Michel Thiebaut de Schotten
    7. Patrick Friedrich
    8. Zhiqiang Sha
    9. Clyde Francks
    10. Espen M Eilertsen
    11. Yunpeng Wang
    12. Kristine B Walhovd
    13. Anders M Fjell
    14. René Westerhausen

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      Roe et al. provide a large-sample analysis of hemispheric lateralisation in brain structure, synthesising local cortical thickness and surface area data from 7 different datasets. The study provides a rich descriptive catalogue of phenomena related to hemispheric anatomical asymmetries. These results are convincing and will prove an important point of reference to neuroscientists who might want to compare their own future results to the ones from this large and varied data set.

    Reviewed by eLife