eLife

Latest preprint reviews

1. NAD+ boosting by oral nicotinamide mononucleotide administration regulates key metabolic and immune pathways through SIRT1 dependent and independent mechanisms to mitigate diet-induced obesity and dyslipidemia in mice

   This article has 12 authors:

   1. Yasser Majeed
   2. Najeeb M Halabi
   3. Rudolf Engelke
   4. Hina Sarwath
   5. Muna N Al-Noubi
   6. Sunkyu Choi
   7. Aisha Al-Malki
   8. Maha V Agha
   9. Muneera Vakayil
   10. Lotfi Chouchane
   11. Frank Schmidt
   12. Nayef A Mazloum

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The mechanistic basis for the potential health benefits of NAD⁺ precursors remains incompletely understood. This manuscript provides a useful assessment of the role of SIRT1 in mediating the effects of NMN in mice fed a high-fat diet. The study addresses a key question, though some of the conclusions appear only partially supported by the presented data.

   Reviewed by eLife

2. Glycolysis-dependent sulfur metabolism orchestrates morphological plasticity and virulence in fungi

   This article has 7 authors:

   1. Dhrumi Shah
   2. Nikita Rewatkar
   3. Adishree M
   4. Siddhi Gupta
   5. Sudharsan Mathivathanan
   6. Sayantani Biswas
   7. Sriram Varahan

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This work identifies a novel, conserved link between glycolysis and sulfur metabolism that governs fungal morphogenesis and virulence. The compelling evidence, integrating multiple approaches, provides an important conceptual advance. A future mechanistic dissection of how sulfur metabolites interface with known pathways is encouraged.

   Reviewed by eLife

3. Ex vivo and in vivo CRISPR/Cas9 screenings identify the roles of protein N-glycosylation in regulating T-cell activation and functions

   This article has 5 authors:

   1. Yu Hong
   2. Xiaofang Si
   3. Wenjing Liu
   4. Xueying Mai
   5. Yu Zhang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The important study uses genome-wide CRISPR/Cas9 screenings to identify a novel target B4GALTZ1 that is implicated in modulating CD8+ T cell function in the context of anti-tumor immunity. The strength of evidence is solid but could benefit from more detail, particularly to verify the efficiency of knockout in their single gene KO lines and identification of N-glycosylation sites of TCR and CD8s. This work highlights the role of protein N-glycosylation, particularly B4GALT1 deficiency, in regulating CD8 function and anti-tumor immunity.

   Reviewed by eLife

4. PTEN restrains SHH medulloblastma growth through cell autonomous and nonautonomous mechanisms

   This article has 5 authors:

   1. Zhimin Lao
   2. Salsabiel El Nagar
   3. Yinwen Liang
   4. Daniel N Stephen
   5. Alexandra L Joyner

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study provides insights into the role of Pten mutations in SHH-medulloblastoma, by using mouse models to resolve the effects of heterozygous vs homozygous mutations on proliferation and cell death throughout tumorigenesis. The experiments presented are convincing, with rigorous quantifications and orthogonal experimentation provided throughout, and the models employing sporadic oncogene induction, rather than EGL-wide genetic modifications, represent an advancement in experimental design. However, additional experimentation focused on a greater characterization of macrophage phenotypes (e.g., microglia vs circulating monocytes) would enhance this study. The work will be of interest to medical biologists studying general cancer mechanisms, as the function of Pten may be similar across tumor types.

   Reviewed by eLife

5. HEB collaborates with TCR signaling to upregulate Id3 and enable γδT17 cell maturation in the fetal thymus

   This article has 12 authors:

   1. Johanna S Selvaratnam
   2. Juliana Dutra Barbosa da Rocha
   3. Vinothkumar Rajan
   4. Helen Wang
   5. Emily C Reddy
   6. Miki S Gams
   7. Jenny Jiahuan Liu
   8. Cornelis Murre
   9. David L Wiest
   10. Cynthia J Guidos
   11. Juan Carlos Zúñiga-Pflücker
   12. Michele K Anderson

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The study provides important mechanistic insight into the transcriptional control of γδT17 development, elegantly demonstrating how HEB and Id3 act sequentially and cooperatively to regulate γδT17 cell specification and maturation. The study provides compelling evidence that advances the understanding of E-Id protein dynamics in thymic T cell specification. The work is comprehensive, technically rigorous, and conceptually clear, and will be of interest to immunologists, developmental biologists, and those studying the molecular underpinnings of physiological outcomes.

   Reviewed by eLife

6. Enhanced Processivity and Collective Force Production of Kinesin-1 at Low Radial Forces

   This article has 2 authors:

   1. Andrew M Hensley
   2. Ahmet Yildiz

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The current work uses DNA-tethered motor trapping to reduce vertical forces and improve datasets for kinesin-1 motility under load. The evidence is compelling and the significance is important to the kinesin field. Kinesin-1 is more robust and less prone to premature detachment than previously indicated. This represents a significant advancement in the field and is generally applicable to work with optical tweezers.

   Reviewed by eLife

7. Cell cycle-dependent cues regulate temporal patterning of the Drosophila central brain neural stem cells

   This article has 2 authors:

   1. Gonzalo N Morales Chaya
   2. Mubarak Hussain Syed

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This manuscript reports important findings indicating that cell cycle progression and cytokinesis both contribute to the transition from early to late neural stem cell fates. Loss-of-function experimental evidence convincingly shows that disrupting the cell cycle or cytokinesis can alter cell fate. This work sets the stage for future investigations into the underlying mechanisms linking the cell cycle to the expression of temporal factors controlling cell fate.

   Reviewed by eLife

8. Microbial consortia in salt marsh sediments are sequentially buried over millennia and genomic complementarity analysis indicates an important role in complex carbon decomposition

   This article has 4 authors:

   1. Joseph H Vineis
   2. Ashley N Bulseco
   3. Zoe G Cardon
   4. Jennifer L Bowen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides a valuable genome-centric characterization of microbial communities across deep sediment cores from a Spartina patens salt marsh. The study provides claims on the metabolic capabilities of the deep sediment microbiome as well as on a burial microbial assembly process and functional complementarity at depth. However, some of these claims remain incomplete and would benefit from further supporting evidence. Overall, this work will be of interest to microbial ecologists working on wetlands.

   Reviewed by eLife

9. Extracting Value Coding Features from Individual Serotonin Neurons

   This article has 3 authors:

   1. Emerson F Harkin
   2. Jean-Claude Béïque
   3. Richard Naud

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The authors used a Bayesian modeling framework to fit behavior and serotonin neuron activity to reward history across multiple timescales. A key goal was to distinguish value coding from other influences, particularly thirst, by comparing model fits across neurons. Although the question and approach are valuable, several limitations of the current manuscript mean that support for the conclusions is incomplete.

   Reviewed by eLife

10. Identifying regulators of associative learning using a protein-labelling approach in Caenorhabditis elegans

    This article has 8 authors:

    1. Aelon Rahmani
    2. Anna McMillen
    3. Ericka Allen
    4. Radwan Ansaar
    5. Renee Green
    6. Michaela E Johnson
    7. Anne Poljak
    8. Yee Lian Chew

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      In reporting on a valuable "learning proteome" for a C. elegans gustatory associative learning paradigm, this work identifies a new set of genes to be tested for roles in learning and memory, describes molecular pathways involving these genes and relevant for learning and memory in C. elegans, and deliver a new set of tools for prodding worm behavior. The methods and results convincingly support the findings, which will be of interest to neuroscientists and developmental biologists seeking to understand the self-assembly and operation of neural circuits for learning and memory.

      [Editors' note: this paper was reviewed by Review Commons.]

    Reviewed by eLife, Review Commons