Latest preprint reviews

1. Single-cell characterization of menstrual fluid at homeostasis and in endometriosis

   This article has 10 authors:

   1. Petra C Schwalie
   2. Cemsel Bafligil
   3. Julie Russeil
   4. Magda Zachara
   5. Marjan Biocanin
   6. Daniel Alpern
   7. Evelin Aasna
   8. Bart Deplancke
   9. Geraldine Canny
   10. Angela Goncalves

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This basic research study presents useful data concerning the menstrual fluid composition and its potential for endometriosis biomarker research. However, despite solid bioinformatics analyses, the choice of markers used to separate or identify the different cell types needs to be justified and the results better discussed in relation to current knowledge of the pathophysiology of endometriosis.

   Reviewed by eLife

2. Tuberculosis susceptibility in genetically diverse mice reveals functional diversity of neutrophils

   This article has 14 authors:

   1. Mariëtta M Ravesloot-Chávez
   2. Erik Van Dis
   3. Douglas Fox
   4. Andrea Anaya Sanchez
   5. Scott Espich
   6. Xammy H Nguyenla
   7. Sagar L Rawal
   8. Helia Samani
   9. Mallory A Ballinger
   10. Henry Thomas
   11. Dmitri Kotov
   12. Russell Vance
   13. Michael W Nachman
   14. Sarah A Stanley

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides valuable insights into the host's variable susceptibility to Mycobacterium tuberculosis, using a novel collection of wild-derived inbred mouse lines from diverse geographic locations, along with immunological and single-cell transcriptomic analyses. While the data are convincing, a deeper mechanistic investigation into neutrophil subset functions would have further enhanced the study. This work will interest microbiologists and immunologists in the TB field.

   Reviewed by eLife

3. Proactive distractor suppression in early visual cortex

   This article has 3 authors:

   1. David Richter
   2. Dirk van Moorselaar
   3. Jan Theeuwes

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This well-written report uses functional neuroimaging in human observers to provide convincing evidence that activity in the early visual cortex is suppressed at locations that are frequently occupied by a task-irrelevant but salient item. This suppression appears to be general to any kind of stimulus, and also occurs in advance of any item actually appearing. The work in its present form will be valuable to those examining attention, perception, learning and prediction, but with a few additional analyses could more informatively rule out potential alternative hypotheses. Further discussion of the mechanistic implications could clarify further the broad extent of its significance.

   Reviewed by eLife

4. The ESCRT protein CHMP5 restricts bone formation by controlling endolysosome-mitochondrion-mediated cell senescence

   This article has 10 authors:

   1. Fan Zhang
   2. Yuan Wang
   3. Luyang Zhang
   4. Chunjie Wang
   5. Deping Chen
   6. Haibo Liu
   7. Ren Xu
   8. Cole M Haynes
   9. Jae-Hyuck Shim
   10. Xianpeng Ge

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important work advances our understanding of CHMP5's role in regulating osteogenesis through its impact on cellular senescence. The evidence supporting the conclusion is mostly convincing, although including additional experiments and discussions would further strengthen the study. This paper holds potential interest for skeletal biologists who study the pathogenesis of age-associated skeletal disorders.

   Reviewed by eLife

5. MorphoCellSorter: An Andrews plot-based sorting approach to rank microglia according to their morphological features

   This article has 17 authors:

   1. Sarah Benkeder
   2. Son-Michel Dinh
   3. Paul Marchal
   4. Priscille De Gea
   5. Muriel Thoby-Brisson
   6. Violaine Hubert
   7. Ines Hristovska
   8. Gabriel Pitollat
   9. Kassandre Combet
   10. Laura Cardoit
   11. Bruno Pillot
   12. Christelle Léon
   13. Marlène Wiart
   14. Serge Marty
   15. Jérôme Honnorat
   16. Olivier Pascual
   17. Jean-Christophe Comte

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The study describes a useful tool for assessing microglia morphology in a variety of experimental conditions. The MorphoCellSorter provides a solid platform for ranking microglia to reflect their morphology continuum and may offer new insight into changes in morphology associated with injury or disease. While the study provides an alternative approach to existing methods for measuring microglia morphology, the functional significance of measured morphological changes remains unclear.

   Reviewed by eLife

6. Cellular evolution of the hypothalamic preoptic area of behaviorally divergent deer mice

   This article has 5 authors:

   1. Jenny Chen
   2. Phoebe R Richardson
   3. Christopher Kirby
   4. Sean R Eddy
   5. Hopi E Hoekstra

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study identifies species- and sex-specific neuronal cell types and gene expression in the medial preoptic area (MPOA) to help understand the evolutionary divergence of social behaviors. The evidence from single-nucleus RNA sequencing and immunostaining is convincing and suggests that cellular differences in the MPOA may contribute to behavioral variations such as mating and parental care that are apparent in two closely related deer mouse species. These rich observations provide an entry point for future hypothesis-driven experiments to demonstrate a causal role for these populations in sex- or species-variable behaviors in vertebrates. These data will be a resource that is of value to behavioral neuroscientists.

   Reviewed by eLife

7. Molecular dynamics simulations illuminate the role of sequence context in the ELF3-PrD-based temperature sensing mechanism in plants

   This article has 5 authors:

   1. Richard J Lindsay
   2. Rafael Giordano Viegas
   3. Vitor BP Leite
   4. Philip A Wigge
   5. Sonya M Hanson

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     In this potentially valuable computational study, the authors conducted atomistic and coarse-grained simulations to probe the temperature-dependent phase behaviors of ELF3, a disordered component of the evening complex in plant. The results aim to highlight the role of polyQ tracts in modulating the temperature sensitivity. The level of evidence is considered incomplete, due to the lack of systematic calibration of the coarse-grained model and limited statistical uncertainty analysis, especially considering the relatively subtle nature of the differences due to temperature change.

   Reviewed by eLife

8. Integrated Analysis of Single-Cell and Bulk RNA-Seq Data reveals that Ferroptosis-Related Genes Mediated the Tumor Microenvironment predicts Prognosis, and guides Drug Selection in Triple-Negative Breast Cancer

   This article has 7 authors:

   1. Xuantong Gong
   2. Lishuang Gu
   3. Di Yang
   4. Yu He
   5. Qian Li
   6. Hao Qin
   7. Yong Wang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents a useful finding on the ferroptosis-mediated tumor microenvironment (TME) in triple-negative breast cancer (TNBC) using public single-cell RNA sequencing (scRNA-seq) and bulk RNA sequencing data. The evidence supporting the claims of the authors is somewhat incomplete and some data are rather questionable; the authors should clarify the relations between ferroptosis-related genes in immune cells and those genes applied in a risk factor analysis in tumor cells. Moreover, the authors should provide experimental validation for the risk score model based on ferroptosis-related genes. The work will be of interest to scientists or clinical scientists working in the field of breast cancer.

   Reviewed by eLife

9. In silico screening by AlphaFold2 program revealed the potential binding partners of nuage-localizing proteins and piRNA-related proteins

   This article has 8 authors:

   1. Kawaguchi Shinichi
   2. Xu Xin
   3. Soga Takashi
   4. Yamaguchi Kenta
   5. Kawasaki Ryuuya
   6. Shimouchi Ryota
   7. Date Susumu
   8. Kai Toshie

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This useful study employs AlphaFold2 to predict interactions among 20 nuage proteins, identifying five novel interaction candidates, three of which are validated experimentally through co-immunoprecipitation. Expanding the analysis to 430 oogenesis-related proteins and screening ~12,000 Drosophila proteins for interactions with Piwi, the study identifies 164 potential binding partners, demonstrating how computational predictions can streamline experimental validation. This study provides a solid basis for further investigations into eukaryotic protein interaction networks.

   Reviewed by eLife

10. Auditory Cortex Learns to Discriminate Audiovisual Cues through Selective Multisensory Enhancement

    This article has 5 authors:

    1. Song Chang
    2. Beilin Zheng
    3. Les Keniston
    4. Jinghong Xu
    5. Liping Yu

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This is an important study that aims to investigate the behavioral relevance of multisensory responses recorded in the auditory cortex. The experiments are elegant and well-designed, and are supported by appropriate analyses of the data. However, the evidence presented for learning-dependent encoding of visual information is incomplete and it is possible that the surprisingly short-latency increases in activity are actually motor-related signals. Demonstrating that they really are visual responses is necessary in order to draw definitive conclusions from this study.

    Reviewed by eLife