eLife

Latest preprint reviews

1. The mechanism of DRB7.2:DRB4 mediated sequestering of endogenous inverted-repeat dsRNA precursors in plants

   This article has 7 authors:

   1. Sneha Paturi
   2. Debadutta Patra
   3. Priti Chanda Behera
   4. Ramdas Aute
   5. Nilam Waghela
   6. Priyadarshan Kinatukara
   7. Mandar V Deshmukh

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The manuscript provides valuable findings in the field for understanding the RNAi regulation in plants at the molecular level with a model of how DRB7.2 and DRB4 form a heterodimer and protect dsRNA from DICER activity. The presented data provide a solid basis for the model, but certain measurements could benefit from replicates for robust statistics.

   Reviewed by eLife

2. Navigating contradictions: Salmonella Typhimurium chemotactic responses to conflicting effector stimuli

   This article has 5 authors:

   1. Kailie Franco
   2. Zealon Gentry-Lear
   3. Michael Shavlik
   4. Michael J Harms
   5. Arden Baylink

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     In this manuscript, Franco and colleagues describe valuable findings about the chemotactic response of Salmonella to serine and indole, conflicting chemotactic signals. Although the evidence presented is solid, concerns were raised about the novelty of the chemotactic phenomena observed with these two compounds. Also, although the induction of invasion by feces is a novel and interesting finding, the lack of follow-up to this observation was also noted.

   Reviewed by eLife

3. C-terminal tagging, transmembrane domain hydrophobicity, and an ER retention motif influence the secretory trafficking of the inner nuclear membrane protein emerin

   This article has 4 authors:

   1. Jessica Mella
   2. Regan Volk
   3. Balyn Zaro
   4. Abigail Buchwalter

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This solid work, a Research Advance linked to Buchwalter et al., 2019, demonstrates that epitope tagging influences protein fate, serving as a cautionary example of how different tagging and imaging strategies may alter the pattern of endogenous protein trafficking. The information presented will be useful for researchers in the field of membrane trafficking, particularly in guiding their experimental designs. That being said, the study offers limited new insights into the biogenesis or disposal of endogenous Emerin.

   Reviewed by eLife

4. How bats exit a crowded colony when relying on echolocation only - a modeling approach

   This article has 2 authors:

   1. Omer Mazar
   2. Yossi Yovel

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This potentially important model-based study seeks to mimic bat echolocation behavior and flight under high-density conditions. The simulations convincingly suggest that the problem of acoustic jamming in these situations may be less severe than previously thought, a finding that would be of broad interest to scientists working in the fields of bat biology and collective behaviour. However, some aspects of the manuscript were found to lack clarity and concerns were raised about some of the assumptions underlying the parameters used for the simulations, which impact both the modeling results and the conclusions that can be made from the data.

   Reviewed by eLife

5. MARK2 regulates Golgi apparatus reorientation by phosphorylation of CAMSAP2 in directional cell migration

   This article has 11 authors:

   1. Peipei Xu
   2. Rui Zhang
   3. Zhengrong Zhou
   4. Honglin Xu
   5. Yuejia Li
   6. Mengge Yang
   7. Ruifan Lin
   8. Yingchun Wang
   9. Xiahe Huang
   10. Qi Xie
   11. Wenxiang Meng

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The authors propose that the kinase MARK2 regulates the Golgi's reorientation towards the cell's leading edge through the regulation of microtubule binding protein CAMSAP2 and its binding to USO1. While the model is interesting and the study is useful, the quantification of an insufficient number of cells and insufficient description of the methods and biological replicates mean the results are inadequate to support the model.

     [Editors' note: this paper was reviewed by Review Commons.]

   Reviewed by eLife

6. Kinetic regulation of kinesin’s two motor domains coordinates its stepping along microtubules

   This article has 5 authors:

   1. Yamato Niitani
   2. Kohei Matsuzaki
   3. Erik Jonsson
   4. Ronald D Vale
   5. Michio Tomishige

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides compelling evidence that kinesin's stepping mechanism is governed by strain-induced conformational changes in its nucleotide-binding pockets. Using pre-steady state kinetics and single-molecule assays, the authors demonstrate that the neck linker's conformation differentially modulates nucleotide affinity and detachment rates, establishing an asynchronous chemo-mechanical cycle that prevents simultaneous detachment. Supported by cryo-EM structural data, the work presents an important advance in our understanding of kinesin's hand-over-hand movement.

     [Editors' note: this paper was reviewed by Review Commons.]

   Reviewed by eLife, Review Commons

7. The title and authors for this article are not available from our external data provider yet:
   10.48550/arxiv.2303.14159v8

8. Movie reconstruction from mouse visual cortex activity

   This article has 3 authors:

   1. Joel Bauer
   2. Troy W Margrie
   3. Claudia Clopath

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study uses state-of-the-art neural encoding and video reconstruction methods to achieve a substantial improvement in video reconstruction quality from mouse neural data, providing a convincing demonstration of how reconstruction performance can be improved by combining these methods. The findings showed that model ensembling and the number of neurons used for reconstruction were key determinants of reconstruction accuracy, but the theoretical contribution to understanding neural encoding was less clear. The treatment of how image masking improved reconstruction performance was also incomplete.

   Reviewed by eLife

9. The C3-C3aR axis modulates trained immunity in alveolar macrophages

   This article has 10 authors:

   1. Alexander P. Earhart
   2. Rafael Aponte Alburquerque
   3. Marick Starick
   4. Aasritha Nallapu
   5. Lorena Garnica
   6. Ayse Naz Ozanturk
   7. Rahul Kumar Maurya
   8. Xiaobo Wu
   9. Jeffrey A. Haspel
   10. Hrishikesh S. Kulkarni

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study explores how complement protein C3 and its signalling may modulate immune training in alveolar macrophages. The findings are an important contribution to the field of trained immunity. The data presented is mainly solid, but incomplete in parts.

   Reviewed by eLife

10. Contributions of insula and superior temporal sulcus to interpersonal guilt and responsibility in social decisions

    This article has 6 authors:

    1. Maria Gädeke
    2. Tom Willems
    3. Omar Salah Ahmed
    4. Bernd Weber
    5. René Hurlemann
    6. Johannes Schultz

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This manuscript provides valuable novel insights into the role of interpersonal guilt in social decision-making by showing that responsibility for a partner's bad lottery outcomes influences happiness. Through the integration of neuroimaging and computational modelling methods, and by combining findings from two studies, the authors provide solid support for their claims.

    Reviewed by eLife