eLife

Latest preprint reviews

1. Passive muscle forces in Drosophila are large but insufficient to support a fly’s weight

   This article has 7 authors:

   1. Ninghan Wang
   2. Helene Babski
   3. Jonathan Elliot Perdomo
   4. Sarah Beth McMahan
   5. Arun Ramakrishnan
   6. Tirthabir Biswas
   7. Vikas Bhandawat

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study introduces a novel experimental and modeling framework to quantify passive joint torques in Drosophila, revealing that passive forces are insufficient to support body weight, contrary to prior assumptions based on larger insects. The approach is technically impressive, combining genetic silencing, kinematic tracking, and biomechanical modeling. However, the strength of evidence is incomplete, limited by concerns about the specificity of the genetic tools, simplifications in the mechanical model, and limited functional interpretation.

   Reviewed by eLife

2. Longitudinal tracking of neuronal activity from the same cells in the developing brain using Track2p

   This article has 7 authors:

   1. Jure Majnik
   2. Manon Mantez
   3. Sofia Zangila
   4. Stéphane Bugeon
   5. Leo Guignard
   6. Jean-Claude Platel
   7. Rosa Cossart

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study presents a new method for longitudinally tracking cells in two-photon imaging data that addresses the specific challenges of imaging neurons in the developing cortex. It provides compelling evidence demonstrating reliable longitudinal identification of neurons across the second postnatal week in mice. The study should be of interest to development neuroscientists engaged in population-level recordings using two-photon imaging.

   Reviewed by eLife

3. A quantitative pipeline for whole-mount deep imaging and analysis of multi-layered organoids across scales

   This article has 8 authors:

   1. Alice Gros
   2. Jules Vanaret
   3. Valentin Dunsing-Eichenauer
   4. Agathe Rostan
   5. Philippe Roudot
   6. Pierre-François Lenne
   7. Léo Guignard
   8. Sham Tlili

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study introduces a powerful imaging approach that enables deep-tissue visualization in gastruloids using two-photon microscopy, combined with spectral imaging and unmixing to achieve four-color 3D image acquisition. The evidence is compelling that many of the established methods are very helpful (e.g., registration, corrections, signal normalisation, lazy loading bioimage visualisation, spectral decomposition analysis), facilitate the development of quantitative research, and would be of interest to the wider scientific community.

   Reviewed by eLife

4. SETD2 suppresses tumorigenesis in a KRAS ^G12C -driven lung cancer model and its catalytic activity is regulated by histone acetylation

   This article has 11 authors:

   1. Ricardo J. Mack
   2. Natasha M. Flores
   3. Geoffrey C. Fox
   4. Hanyang Dong
   5. Metehan Cebeci
   6. Simone Hausmann
   7. Tourkian Chasan
   8. Jill M. Dowen
   9. Brian D. Strahl
   10. Pawel K. Mazur
   11. Or Gozani

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This is an important study providing molecular insight into how cross-talk between histone modifications regulates the histone H3K36 methyltransferase SETD2. The manuscript contains excellent quality data, and the conclusions are convincing and justified. This work will be of interest to many biochemists working in the field of chromatin biology and epigenetics.

   Reviewed by eLife

5. Mechanism of SK2 channel gating and its modulation by the bee toxin apamin and small molecules

   This article has 10 authors:

   1. Samantha J Cassell
   2. Weiyan Li
   3. Simon Krautwald
   4. Maryam Khoshouei
   5. Yan Tony Lee
   6. Joyce Hou
   7. Wendy Guan
   8. Stefan Peukert
   9. Wilhelm A Weihofen
   10. Jonathan R Whicher

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     In this important manuscript, Cassell and colleagues set out on a mechanistic and pharmacological exploration of an engineered chimeric small conductance calcium-activated potassium channel 2 (SK2). They show convincing evidence that the SK2 channel possesses a unique extracellular structure that modulates the conductivity of the selectivity filter, and that this structure is the target for the SK2 inhibitor apamin. While the interpretations are sound and the writing is clear, the manuscript would be strengthened by providing more detailed information for the electrophysiological experiments and the structural analyses attempted, in addition to relating dilation of the filter to mechanisms of inactivation in other potassium channels. This high-quality study will be of interest to membrane protein structural biologists, ion channel biophysicists, and chemical biologists, and will inform future drug development targeting SK channels.

   Reviewed by eLife

6. Multiple pathways prevent bi-parental mitochondria transmission in C. elegans

   This article has 5 authors:

   1. Valentine Melin
   2. Justine Cailloce
   3. Fanny Husson
   4. Jorge Merlet
   5. Vincent Galy

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable work used molecular biology, cell biology, and genetic approaches to unravel individual genes and potential pathways that contribute to paternal mitochondrial inheritance using C. elegans as the model organism. Their microscopy method is cutting edge, with sufficient biological replicates, proper control, and appropriate statistics. These findings are convincing and are of general interest for understanding mitochondrial inheritance in C. elegans, which could have implications for understanding similar biological processes in other organisms.

   Reviewed by eLife

7. A high-resolution analysis of arrestin2 interactions responsible for CCR5 endocytosis

   This article has 6 authors:

   1. Ivana Petrovic
   2. Samit Desai
   3. Polina Isaikina
   4. Layara Akemi Abiko
   5. Anne Spang
   6. Stephan Grzesiek

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The authors investigate arrestin2-mediated CCR5 endocytosis in the context of clathrin and AP2 contributions. Using an extensive set of NMR experiments, and supported by microscopy and other biophysical assays, the authors provide convincing data on the roles of AP2 and clathrin in CCR5 endocytosis. This important work will appeal to an audience beyond those studying chemokine receptors, including those studying GPCR regulation and trafficking. The distinct role of AP2 and not clathrin will be of particular interest to those studying GPCR internalization mechanisms.

   Reviewed by eLife

8. Long-term live imaging, cell identification and cell tracking in regenerating crustacean legs

   This article has 8 authors:

   1. Çağrı Çevrim
   2. Béryl Laplace-Builhé
   3. Ko Sugawara
   4. Maria Lorenza Rusciano
   5. Nicolas Labert
   6. Jacques Brocard
   7. Alba Almazán
   8. Michalis Averof

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents a valuable technical advance in the long-term live imaging of limb regeneration at cellular resolution in Parhyale hawaiensis. The authors develop and carefully validate a method to continuously image entire regenerating legs over several days while minimizing photodamage and optimizing conditions for robust cell tracking, together with post-hoc in situ identification of cell types. The data are convincing, the methodology is rigorous and clearly documented, and the results will be of interest to researchers in regeneration biology, developmental biology, and advanced live imaging and cell tracking software development.

     [Editors' note: this paper was reviewed by Review Commons.]

   Reviewed by eLife, Review Commons

9. Xcr1 ⁺ type 1 conventional dendritic cells are essential mediators for atherosclerosis progression

   This article has 17 authors:

   1. Tianhan Li
   2. Liaoxun Lu
   3. Juanjuan Qiu
   4. Xin Dong
   5. Le Yang
   6. Kexin He
   7. Yanrong Gu
   8. Binhui Zhou
   9. Tingting Jia
   10. Toby Lawrence
   11. Marie Malissen
   12. Guixue Wang
   13. Rong Huang
   14. Hui Wang
   15. Bernard Malissen
   16. Yinming Liang
   17. Lichen Zhang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This manuscript by Li, Lu et al., presents important findings on the role of cDC1 in atherosclerosis and their influence on the adaptive immune system. Using Xcr1Cre-Gfp Rosa26LSL-DTA ApoE-/- mouse models, these data convincingly reveal an unexpected, non-redundant role of the XCL1-XCR1 axis in mediating cDC1 contributions to atherosclerosis.

   Reviewed by eLife

10. An abundant merozoite surface protein of Plasmodium falciparum modulates susceptibility to inhibitory antibodies

    This article has 11 authors:

    1. Isabelle G Henshall
    2. Jill Chmielewski
    3. Dimuthu Angage
    4. Ornella Romeo
    5. Keng Heng Lai
    6. Kaitlin R Turland
    7. Nicki Badii
    8. Michael Foley
    9. Robin F Anders
    10. James Beeson
    11. Danny W Wilson

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This important work offers a fresh perspective central to merozoite surface biology and potential implications on vaccine design, challenging the dogma that MSPs are indispensable invasion engines. Although the authors only deleted bp 132-819, the data based on Western blot, IFA, and RNA‐seq provide compelling evidence that while MSP2 is dispensable for growth, it serves as an immune modulator for AMA1. This work will be of particular interest to scientists working on different aspects of Plasmodium biology and vaccinology.

    Reviewed by eLife