eLife

Latest preprint reviews

1. A scalable and tunable platform for functional interrogation of peptide hormones in fish

   This article has 3 authors:

   1. Eitan Moses
   2. Roman Franek
   3. Itamar Harel

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     Moises and Harel generate an important set of novel molecular tools in African turquoise killifish, an innovative model to study aging biology. The new solid tools described in this paper can boost this buddying model system for broad biotechnological applications. The authors showcase the efficacy of their tools in the context of peptide hormones involved in growth and gonad development. The killifish community will greatly benefit from these novel tools and the relevance of the developed methods will likely go beyond the killifish community.

   Reviewed by eLife

2. Diverse evolutionary pathways challenge the use of collateral sensitivity as a strategy to suppress resistance

   This article has 10 authors:

   1. Rebecca EK Mandt
   2. Madeline R Luth
   3. Mark A Tye
   4. Ralph Mazitschek
   5. Sabine Ottilie
   6. Elizabeth A Winzeler
   7. Maria Jose Lafuente-Monasterio
   8. Francisco Javier Gamo
   9. Dyann F Wirth
   10. Amanda K Lukens

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study addresses an important question in the field of antimicrobial chemotherapy: whether combinations of enzyme inhibitors that select for mutations that confer resistance to one inhibitor and at the same time increased sensitization to the other inhibitor can provide a path towards mitigating resistance risks. The authors here investigated one such combination of inhibitors of Plasmodium falciparum DHODH (dihydroorotate dehydrogenase), finding that despite "collateral sensitivity", it was still possible to select a mutation that mediated resistance to both inhibitors without any change in parasite fitness. Additional cross-susceptibility and structural modeling strengthen this study, which is performed to a high technical standard and presents a convincing body of data.

   Reviewed by eLife

3. Candida albicans exhibits heterogeneous and adaptive cytoprotective responses to antifungal compounds

   This article has 13 authors:

   1. Vanessa Dumeaux
   2. Samira Massahi
   3. Van Bettauer
   4. Austin Mottola
   5. Anna Dukovny
   6. Sanny Singh Khurdia
   7. Anna Carolina Borges Pereira Costa
   8. Raha Parvizi Omran
   9. Shawn Simpson
   10. Jinglin Lucy Xie
   11. Malcolm Whiteway
   12. Judith Berman
   13. Michael T Hallett

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The valuable study by Dumeaux et al examines the transcriptional response to antifungal treatment in the major opportunistic human fungal pathogen Candida albicans. Using solid methodology, including a novel droplet-based single cell transcriptomics platform, the authors report that fungal cells exhibit heterogeneity in their transcriptional response to antifungal drug treatment. The ability to study the trajectories of individual cells in a high-throughput manner provides a novel perspective on studying the emergence of drug tolerance and resistance in fungal pathogens.

   Reviewed by eLife

4. Resting-state fMRI signals contain spectral signatures of local hemodynamic response timing

   This article has 4 authors:

   1. Sydney M Bailes
   2. Daniel EP Gomez
   3. Beverly Setzer
   4. Laura D Lewis

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This manuscript addresses the important issue of hemodynamic response function (HRF) variability across brain areas and will be valuable to researchers who use fMRI and other types of functional imaging that rely on neurovascular coupling. Using simulations and experiments, the authors provide solid evidence that differences in the HRF can impact spectrum-based metrics such as ALFF and fALFF. A better understanding of the variability of the HRF is critical for the proper interpretation of activation onset times and of differences observed in clinical populations where both neural and vascular alterations can be expected.

   Reviewed by eLife

5. Rab12 is a regulator of LRRK2 and its activation by damaged lysosomes

   This article has 16 authors:

   1. Xiang Wang
   2. Vitaliy V Bondar
   3. Oliver B Davis
   4. Michael T Maloney
   5. Maayan Agam
   6. Marcus Y Chin
   7. Audrey Cheuk-Nga Ho
   8. Rajarshi Ghosh
   9. Dara E Leto
   10. David Joy
   11. Meredith EK Calvert
   12. Joseph W Lewcock
   13. Gilbert Di Paolo
   14. Robert G Thorne
   15. Zachary K Sweeney
   16. Anastasia G Henry

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This valuable study shows that Rab12 is required for LRRK2 activation. However, while some of the data are compelling, some claims, especially the ones related to LRRK2's membrane association are not supported. Addressing discrepancies between figures (pointed out by reviewers) and re-writing certain sections will greatly improve this manuscript.

   Reviewed by eLife

6. The Reissner fiber under tension in vivo shows dynamic interaction with ciliated cells contacting the cerebrospinal fluid

   This article has 8 authors:

   1. Celine Bellegarda
   2. Guillaume Zavard
   3. Lionel Moisan
   4. Françoise Brochard-Wyart
   5. Jean-François Joanny
   6. Ryan S Gray
   7. Yasmine Cantaut-Belarif
   8. Claire Wyart

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This exceptional work substantially advances our understanding of the mechanics of the Reissner's fibre (RF) by performing in-vivo experiments that track and analyze the behavior of the RF when it is cut and the behavior of ciliated cells touching the RF when contact is interrupted. The data is valuable and the conclusions are compelling. The work will be of broad interest to many research communities including developmental neuroscience and cilia biology.

   Reviewed by eLife

7. CaMKII autophosphorylation can occur between holoenzymes without subunit exchange

   This article has 10 authors:

   1. Iva Lučić
   2. Léonie Héluin
   3. Pin-Lian Jiang
   4. Alejandro G Castro Scalise
   5. Cong Wang
   6. Andreas Franz
   7. Florian Heyd
   8. Markus C Wahl
   9. Fan Liu
   10. Andrew JR Plested

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This manuscript reports the fundamental finding that an oligomeric protein kinase, CaMKII, can be phosphorylated by another molecule of the holoenzyme in a manner that does not involve subunit exchange. The evidence for the main conclusion is compelling, supported by several independent experiments. If independently confirmed in future, the study will stand as having provided a novel regulatory mechanism for the autophosphorylation of this kinase. The work will be of broad interest to molecular and cellular neuroscientists as well as biochemists.

   Reviewed by eLife

8. Sleep spindle maturity promotes slow oscillation-spindle coupling across child and adolescent development

   This article has 5 authors:

   1. Ann-Kathrin Joechner
   2. Michael A Hahn
   3. Georg Gruber
   4. Kerstin Hoedlmoser
   5. Markus Werkle-Bergner

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This is an important analysis of two sleep datasets in children and adolescents that contributes to our understanding of sleep spindle and slow oscillation dynamics during development and is expected to be of interest to interdisciplinary fields including development and sleep. The analyses are solid and adequately complex to capture the changes in sleep spindle to slow oscillation coupling between the age groups. However, the paper would be strengthened by performing the same analyses in an adult sample to sufficiently characterize the maturation of sleep spindles and their coupling to slow oscillations.

   Reviewed by eLife

9. Risk of second primary cancers after a diagnosis of first primary cancer: A pan-cancer analysis and Mendelian randomization study

   This article has 8 authors:

   1. Xiaohao Ruan
   2. Da Huang
   3. Yongle Zhan
   4. Jingyi Huang
   5. Jinlun Huang
   6. Ada Tsui-Lin Ng
   7. James Hok-Leung Tsu
   8. Rong Na

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study presents a valuable finding on the associations and causal relationship between second primary cancers and the initial diagnosis of a primary cancer via using a large database. The evidence supporting the claims of the authors is solid. The work will be of interest to cancer clinicians.

   Reviewed by eLife

10. Genetically engineered mesenchymal stem cells as a nitric oxide reservoir for acute kidney injury therapy

    This article has 10 authors:

    1. Haoyan Huang
    2. Meng Qian
    3. Yue Liu
    4. Shang Chen
    5. Huifang Li
    6. Zhibo Han
    7. Zhong-Chao Han
    8. Xiang-Mei Chen
    9. Qiang Zhao
    10. Zongjin Li

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      This important study combines engineered mesenchymal stem cells together with mouse models of kidney injury to determine the ability of these cells to reduce kidney damage upon acute kidney injury. The evidence supporting the claims is solid, although the inclusion of more than one type of stem cell and the use of male mice which are more prone to acute kidney injury, would strengthen the study. This work will be of interest to both basic scientists and clinicians working on mechanisms of kidney injury and repair.

    Reviewed by eLife