eLife

Latest preprint reviews

1. Vibrio cholerae’s ToxRS bile sensing system

   This article has 15 authors:

   1. Nina Gubensäk
   2. Theo Sagmeister
   3. Christoph Buhlheller
   4. Bruno Di Geronimo
   5. Gabriel E Wagner
   6. Lukas Petrowitsch
   7. Melissa A Gräwert
   8. Markus Rotzinger
   9. Tamara M Ismael Berger
   10. Jan Schäfer
   11. Isabel Usón
   12. Joachim Reidl
   13. Pedro A Sánchez-Murcia
   14. Klaus Zangger
   15. Tea Pavkov-Keller

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study provides important insights into the structural biology and molecular mechanism of the sensory proteins ToxR/S that are associated with survival and virulence of the cholera pathogen. The structural studies are solid and supported by a series of biophysical experiments revealing a split, periplasmic protein binding interface for bile acid. The results are of interest to both protein biochemistry and pharmacology, potentially opening new routes for intervention in cholera disease.

   Reviewed by eLife

2. Stable population structure in Europe since the Iron Age, despite high mobility

   This article has 97 authors:

   1. Margaret L Antonio
   2. Clemens L Weiß
   3. Ziyue Gao
   4. Susanna Sawyer
   5. Victoria Oberreiter
   6. Hannah M Moots
   7. Jeffrey P Spence
   8. Olivia Cheronet
   9. Brina Zagorc
   10. Elisa Praxmarer
   11. Kadir Toykan Özdoğan
   12. Lea Demetz
   13. Pere Gelabert
   14. Daniel Fernandes
   15. Michaela Lucci
   16. Timka Alihodžić
   17. Selma Amrani
   18. Pavel Avetisyan
   19. Christèle Baillif-Ducros
   20. Željka Bedić
   21. Audrey Bertrand
   22. Maja Bilić
   23. Luca Bondioli
   24. Paulina Borówka
   25. Emmanuel Botte
   26. Josip Burmaz
   27. Domagoj Bužanić
   28. Francesca Candilio
   29. Mirna Cvetko
   30. Daniela De Angelis
   31. Ivan Drnić
   32. Kristián Elschek
   33. Mounir Fantar
   34. Andrej Gaspari
   35. Gabriella Gasperetti
   36. Francesco Genchi
   37. Snežana Golubović
   38. Zuzana Hukeľová
   39. Rimantas Jankauskas
   40. Kristina Jelinčić Vučković
   41. Gordana Jeremić
   42. Iva Kaić
   43. Kevin Kazek
   44. Hamazasp Khachatryan
   45. Anahit Khudaverdyan
   46. Sylvia Kirchengast
   47. Miomir Korać
   48. Valérie Kozlowski
   49. Mária Krošláková
   50. Dora Kušan Špalj
   51. Francesco La Pastina
   52. Marie Laguardia
   53. Sandra Legrand
   54. Tino Leleković
   55. Tamara Leskovar
   56. Wiesław Lorkiewicz
   57. Dženi Los
   58. Ana Maria Silva
   59. Rene Masaryk
   60. Vinka Matijević
   61. Yahia Mehdi Seddik Cherifi
   62. Nicolas Meyer
   63. Ilija Mikić
   64. Nataša Miladinović-Radmilović
   65. Branka Milošević Zakić
   66. Lina Nacouzi
   67. Magdalena Natuniewicz-Sekuła
   68. Alessia Nava
   69. Christine Neugebauer-Maresch
   70. Jan Nováček
   71. Anna Osterholtz
   72. Julianne Paige
   73. Lujana Paraman
   74. Dominique Pieri
   75. Karol Pieta
   76. Stefan Pop-Lazić
   77. Matej Ruttkay
   78. Mirjana Sanader
   79. Arkadiusz Sołtysiak
   80. Alessandra Sperduti
   81. Tijana Stankovic Pesterac
   82. Maria Teschler-Nicola
   83. Iwona Teul
   84. Domagoj Tončinić
   85. Julien Trapp
   86. Dragana Vulović
   87. Tomasz Waliszewski
   88. Diethard Walter
   89. Miloš Živanović
   90. Mohamed el Mostefa Filah
   91. Morana Čaušević-Bully
   92. Mario Šlaus
   93. Dušan Borić
   94. Mario Novak
   95. Alfredo Coppa
   96. Ron Pinhasi
   97. Jonathan K Pritchard

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This paper will be of considerable interest to population geneticists and other scholars in the field of paleogenomics. The study provides an impressive dataset containing 200+ novel human ancient genome sequences and a very creative, robust, and novel approach for studying human migration across time using ancient DNA. The authors find that the population structure in Europe has been remarkably stable over time. The conclusions are well supported by the data and the methods used are thoughtful and rigorous.

   Reviewed by eLife

3. Functionally refined encoding of threat memory by distinct populations of basal forebrain cholinergic projection neurons

   This article has 15 authors:

   1. Prithviraj Rajebhosale
   2. Mala R Ananth
   3. Ronald Kim
   4. Richard Crouse
   5. Li Jiang
   6. Gretchen López-Hernández
   7. Chongbo Zhong
   8. Christian Arty
   9. Shaohua Wang
   10. Alice Jone
   11. Niraj S Desai
   12. Yulong Li
   13. Marina R Picciotto
   14. Lorna W Role
   15. David A Talmage

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study examines the existence of a fear memory engram in acetylcholine neurons of the basal forebrain and seeks to link this to modulation of amygdala for fear expression. Using a combination of techniques including genetic access to cFos expressing neurons, in-vivo chemogenetics, and optical detection of acetylcholine (ACh), the authors present solid evidence that posteriorly-located amygdala projecting basal forebrain cholinergic neurons participate in cue-specific threat learning and memory. This paper will be of interest to those studying circuit-level mechanisms of learning and emotion regulation.

   Reviewed by eLife

4. Myristoyl’s dual role in allosterically regulating and localizing Abl kinase

   This article has 2 authors:

   1. Svenja de Buhr
   2. Frauke Gräter

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This is an important study of the mechanism of how binding of the fatty acid myristic acid (MYR) inhibits the activity of the kinase c-Abl, a critical regulator of many cellular processes. While the general aspects of this regulation are known from structure determination and biochemical studies, the exact molecular mechanism and the nature of the allosteric inhibition were not known. The authors use MD simulation to close this gap and provide a detailed mechanistic description of the inhibitory mechanism, although some of the evidence remains incomplete.

   Reviewed by eLife

5. Auditory confounds can drive online effects of transcranial ultrasonic stimulation in humans

   This article has 18 authors:

   1. Benjamin R Kop
   2. Yazan Shamli Oghli
   3. Talyta C Grippe
   4. Tulika Nandi
   5. Judith Lefkes
   6. Sjoerd W Meijer
   7. Soha Farboud
   8. Marwan Engels
   9. Michelle Hamani
   10. Melissa Null
   11. Angela Radetz
   12. Umair Hassan
   13. Ghazaleh Darmani
   14. Andrey Chetverikov
   15. Hanneke EM den Ouden
   16. Til Ole Bergmann
   17. Robert Chen
   18. Lennart Verhagen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important multicenter study provides convincing evidence that the auditory noise emitted during online transcranial ultrasound stimulation (TUS) protocols can pose a considerable confound and is able to explain corticospinal excitability changes as measured with Motor Evoked Potentials (MEP). The findings lay the ground for future studies optimising protocols and control conditions to leverage TUS as a meaningful experimental and clinical tool. A clear strength of the study is the multitude of control conditions (i.e., control sites, acoustic masking, acoustic stimulation). These findings will be of interest to neuroscience researchers using brain stimulation approaches.

   Reviewed by eLife

6. An anciently diverged family of RNA binding proteins maintain correct splicing of a class of ultra-long exons through cryptic splice site repression

   This article has 17 authors:

   1. Chileleko Siachisumo
   2. Sara Luzzi
   3. Saad Aldalaqan
   4. Gerald Hysenaj
   5. Caroline Dalgliesh
   6. Kathleen Cheung
   7. Matthew R Gazzara
   8. Ivaylo D Yonchev
   9. Katherine James
   10. Mahsa Kheirollahi Chadegani
   11. Ingrid E Ehrmann
   12. Graham R Smith
   13. Simon J Cockell
   14. Jennifer Munkley
   15. Stuart A Wilson
   16. Yoseph Barash
   17. David J Elliott

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important paper addresses the process by which cryptic splice sites that occur randomly in exons are ignored by the splicing machinery. Integrating state-of- the-art genome-wide approaches such as CLIP-seq with the study of individual examples, this study convincingly implicates members of RBMX family of RNA binding proteins in such cryptic splice site suppression and showcases its importance for the fidelity of expression of genes with very large exons.

   Reviewed by eLife

7. Conserved regulatory motifs in the juxtamembrane domain and kinase N-lobe revealed through deep mutational scanning of the MET receptor tyrosine kinase domain

   This article has 9 authors:

   1. Gabriella O Estevam
   2. Edmond M Linossi
   3. Christian B Macdonald
   4. Carla A Espinoza
   5. Jennifer M Michaud
   6. Willow Coyote-Maestas
   7. Eric A Collisson
   8. Natalia Jura
   9. James S Fraser

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This manuscript describes a deep mutational scanning study of the kinase domain of the MET receptor tyrosine kinase. The study yields an important catalog of essentially all possible deleterious mutations in this portion of the receptor., with convincing evidence. The manuscript will be of interest to researchers working in the field of receptor tyrosine kinases.

   Reviewed by eLife

8. Hypothalamic corticotropin-releasing hormone neurons modulate sevoflurane anesthesia and the post-anesthesia stress responses

   This article has 5 authors:

   1. Shan Jiang
   2. Lu Chen
   3. Wei-Min Qu
   4. Zhi-Li Huang
   5. Chang-Rui Chen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study presents useful findings for how sevoflurane anesthesia modulates the activity of corticotropin-releasing hormone neurons in the paraventricular nucleus of the hypothalamus and how manipulation of such PVHCRH neurons influences anesthesia and post-anesthesia responses. The technical approaches are solid and the data presented is largely clear. Whether PVHCRH neurons are critical for the mechanisms of sevoflurane anesthesia is a direction for the future.

   Reviewed by eLife

9. CRISPR-Cas9 knockdown of ESR1 in preoptic GABA-kisspeptin neurons suppresses the preovulatory surge and estrous cycles in female mice

   This article has 6 authors:

   1. Jenny Clarkson
   2. Siew Hoong Yip
   3. Robert Porteous
   4. Alexia Kauff
   5. Alison K Heather
   6. Allan E Herbison

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study provides convincing evidence of the criticality of estradiol – estrogen receptor-mediated upregulation of kisspeptin within neurons of the preoptic area to generate an ovulation-inducing luteinizing hormone surge. The use of in vivo CRIPSR-Cas9 is novel in this system and provides a road map for future studies in reproductive neuroendocrinology. This paper will be of interest to reproductive neuroscientists and endocrinologists.

   Reviewed by eLife

10. Neuroendocrine gene expression coupling of interoceptive bacterial food cues to foraging behavior of C. elegans

    This article has 3 authors:

    1. Sonia A Boor
    2. Joshua D Meisel
    3. Dennis H Kim

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      This important manuscript focuses on the mechanisms by which food signals and food ingestion modulate animal foraging. The authors provide convincing support for the interesting idea that chemosensory and interoceptive signals converge on transcriptional regulation of the TGF-beta ligand DAF-7 in a single pair of C. elegans chemosensory neurons (ASJ) to regulate behavior. Their studies implicate a conserved signaling molecule, ALK, in this regulation, suggesting a conserved link between food cues and the neuroendocrine control of foraging behavior.

    Reviewed by eLife