eLife

Latest preprint reviews

1. ZC3H11A mutations cause high myopia by triggering PI3K-AKT and NF-κB-mediated signaling pathway in humans and mice

   This article has 9 authors:

   1. Chong Chen
   2. Qian Liu
   3. Cheng Tang
   4. Yu Rong
   5. Xinyi Zhao
   6. Dandan Li
   7. Fan Lu
   8. Jia Qu
   9. Xinting Liu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This work investigates ZC3H11A as a cause of high myopia through the analysis of human data and experiments with genetic knockout of Zc3h11a in mouse, providing a useful model of myopia. The evidence supporting the conclusion is still incomplete in the revised manuscript as the concerns raised in the previous review were not fully addressed. The article would benefit from a more robust genetic analysis and comprehensive presentation of human phenotypic data to clarify the modes of inheritance in the families, currently limited by loss of patient follow-up and addressing whether there is a reduction in bipolar cell number or decreased marker protein expression through cell counts or quantifiable, less saturated Western blots. The work will be of interest to ophthalmologists and researchers working on myopia

   Reviewed by eLife

2. Cellular characterization of the mouse collecting lymphatic vessels reveals that lymphatic muscle cells are the innate pacemaker cells

   This article has 12 authors:

   1. Scott D Zawieja
   2. Grace A Pea
   3. Sarah E Broyhill
   4. Advaya Patro
   5. Karen H Bromert
   6. Charles E Norton
   7. Hae Jin Kim
   8. Sathesh Kumar Sivasankaran
   9. Min Li
   10. Jorge A Castorena-Gonzalez
   11. Bernard T Drumm
   12. Michael J Davis

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This manuscript aims to identify the pacemaker cells in the lymphatic collecting vessels - the cells that initiate the autonomous action potentials and contractions needed to drive lymphatic pumping. Through the exemplary use of existing approaches (genetic deletions and cytosolic calcium detection in multiple cell types), the authors convincingly determine that lymphatic muscle cells are the origin of the action potential that triggers lymphatic contraction. The inclusion of scRNAseq and membrane potential data enhances a tremendous study. This fundamental discovery establishes a new standard for the field of lymphatic physiology.

   Reviewed by eLife

3. Single-molecule analysis reveals the phosphorylation of FLS2 governs its spatiotemporal dynamics and immunity

   This article has 10 authors:

   1. Yaning Cui
   2. Hongping Qian
   3. Jinhuan Yin
   4. Changwen Xu
   5. Pengyun Luo
   6. Xi Zhang
   7. Meng Yu
   8. Bodan Su
   9. Xiaojuan Li
   10. Jinxing Lin

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This potentially important study employs advanced imaging techniques to directly visualize molecular dynamics and of the immune receptor kinase FLS2 in specific microenvironments. The evidence supporting the ligand-induced association with remorin and the requirement of a previously reported phosphosite as presented is solid, although support by independent methods would be welcome. The work will be of interest to plant biologists working on cell surface receptors.

   Reviewed by eLife

4. Quantitative Geometric Modeling of Blood Cells from X-ray Histotomograms of Whole Zebrafish Larvae

   This article has 12 authors:

   1. Maksim A. Yakovlev
   2. Ke Liang
   3. Carolyn R. Zaino
   4. Daniel J. Vanselow
   5. Andrew L. Sugarman
   6. Alex Y. Lin
   7. Patrick J. La Riviere
   8. Yuxi Zheng
   9. Justin D. Silverman
   10. John C. Leichty
   11. Sharon X. Huang
   12. Keith C. Cheng

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     Tissue phenotyping is central to nearly all areas of biology. In this study, the authors use an advanced form of micro-CT (X-ray histotomography) in zebrafish to phenotype blood cells in the intact animal. These approaches build upon prior work from this group and others showing this is a scalable imaging method that could readily be applied to other cell types, and provide an excellent complement to histological analysis of tissues. This is important work, as it demonstrates that the method can provide an approach that is orthogonal to conventional histology. The strength of the presented data is compelling, with description of both the hardware and software needed to implement the protocol, which will make it accessible to other researchers in the field.

   Reviewed by eLife

5. Novel sterol binding domains in bacteria

   This article has 7 authors:

   1. Liting Zhai
   2. Amber C Bonds
   3. Clyde A Smith
   4. Hannah Oo
   5. Jonathan Chiu-Chun Chou
   6. Paula V Welander
   7. Laura MK Dassama

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This is a valuable contribution to our understanding of how some bacteria can transport sterols from the cytoplasm to the outer membrane. Though much remains to be tested and explored, the data and analyses presented here provide solid evidence for the genetic and physical interaction of BstA/B/C with bacterially-produced sterols. The manuscript will be of interest to scientists focusing on the characterization of novel bacterial proteins and those studying lipid transport and acquisition in bacterial pathogens.

   Reviewed by eLife

6. Shh from mossy cells contributes to preventing NSC pool depletion after seizure-induced neurogenesis and in aging

   This article has 5 authors:

   1. Hirofumi Noguchi
   2. Jessica Chelsea Arela
   3. Thomas Ngo
   4. Laura Cocas
   5. Samuel Pleasure

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study uses specific and robust genetic approaches to assess mechanisms of kainic acid-induced neurogenesis. This is a fundamental study that bridges several complementary methods and is a convincing use of existing approaches to explore roles for sonic hedgehog in activity-dependent and aging-associated hippocampal neurogenesis.

   Reviewed by eLife

7. Ribosome subunit attrition and activation of the p53–MDM4 axis dominate the response of MLL-rearranged cancer cells to WDR5 WIN site inhibition

   This article has 20 authors:

   1. Gregory Caleb Howard
   2. Jing Wang
   3. Kristie L Rose
   4. Camden Jones
   5. Purvi Patel
   6. Tina Tsui
   7. Andrea C Florian
   8. Logan Vlach
   9. Shelly L Lorey
   10. Brian C Grieb
   11. Brianna N Smith
   12. Macey J Slota
   13. Elizabeth M Reynolds
   14. Soumita Goswami
   15. Michael R Savona
   16. Frank M Mason
   17. Taekyu Lee
   18. Stephen Fesik
   19. Qi Liu
   20. William P Tansey

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important paper reveals that one of the major roles of the WDR5 WIN site is to promote ribosome synthesis, and that by attacking the WIN site with inhibitors ribosome attrition occurs creating new vulnerabilities that can be therapeutically exploited. This deficiency of ribosomal proteins also provokes the p53 response. The data from a variety of approaches is generally very convincing, and together buttresses the authors' conclusions and interpretations quite nicely; overall, this paper will provide a justification for pre-clinical and translational studies of WDR5 interaction site inhibitors.

   Reviewed by eLife

8. The pelvic organs receive no parasympathetic innervation

   This article has 13 authors:

   1. Margaux Sivori
   2. Bowen Dempsey
   3. Zoubida Chettouh
   4. Franck Boismoreau
   5. Maïlys Ayerdi
   6. Annaliese Eymael
   7. Sylvain Baulande
   8. Sonia Lameiras
   9. Fanny Coulpier
   10. Olivier Delattre
   11. Hermann Rohrer
   12. Olivier Mirabeau
   13. Jean-François Brunet

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This useful study compares gene expression patterns among different autonomic ganglia and will be of interest to developmental neuroscientists and neurophysiologists. The study expands the database of genes expressed by subpopulations of autonomic neurons in ganglia, a key step in decoding their developmental origins and physiological functions. The evidence supporting the alternative view that the pelvic ganglionic neurons are actually modified sympathetic neurons is incomplete and may cause confusion, given the enrichment of cholinergic neurons, as well as the large number of molecular and functional differences known to be present between cranial and sacral neurons.

   Reviewed by eLife

9. Acute ampakines increase voiding function and coordination in a rat model of SCI

   This article has 5 authors:

   1. Sabhya Rana
   2. Firoj Alom
   3. Robert C Martinez
   4. David D Fuller
   5. Aaron D Mickle

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     Bladder dysfunction following spinal cord injury (SCI) represents a severe and disabling complication without effective therapies. Following evidence that AMPA receptors play a key role in bladder function the authors show convincingly that AMPA allosteric activators can ameliorate many of the subacute defects in bladder and sphincter function following SCI, including prolonged voiding intervals and high bladder pressure thresholds for voiding. These valuable results in rodents may help in the development of these agents as therapeutics for humans with SCI-induced bladder dysfunction.

   Reviewed by eLife

10. Disease modeling and pharmacological rescue of autosomal dominant retinitis pigmentosa associated with RHO copy number variation

    This article has 8 authors:

    1. Sangeetha Kandoi
    2. Cassandra Martinez
    3. Kevin Xu Chen
    4. Miika Mehine
    5. L Vinod K Reddy
    6. Brian C Mansfield
    7. Jacque L Duncan
    8. Deepak A Lamba

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      This study presents an important finding that implicates a rhodopsin gene duplication in the progression of autosomal dominant retinitis pigmentosa in patients. The authors utilize a retinal organoid model to demonstrate a similar disease phenotype and suggest defects can be ameliorated by using photoregulin. The data supporting the conclusions are solid, but there are some concerns regarding the strength of the phenotype in retinal organoids. This work will be of broad interest to vision researchers.

    Reviewed by eLife