eLife

Latest preprint reviews

1. Sorting of secretory proteins at the trans-Golgi network by human TGN46

   This article has 10 authors:

   1. Pablo Lujan
   2. Carla Garcia-Cabau
   3. Yuichi Wakana
   4. Javier Vera Lillo
   5. Carmen Rodilla-Ramírez
   6. Hideaki Sugiura
   7. Vivek Malhotra
   8. Xavier Salvatella
   9. Maria F Garcia-Parajo
   10. Felix Campelo

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study provides the fundamental insight that TGN46, a single-pass membrane protein, acts as a cargo receptor for proteins at the Trans-Golgi Network. The authors demonstrate that the luminal domain of TGN46 is crucial for the incorporation of the soluble secretory protein PAUF into CARTS, a class of vesicles mediating TGN to surface traffic. The data presented are compelling, yielding a clear model for the sorting of cargos destined for secretion.

   Reviewed by eLife

2. Plasma growth hormone pulses induce male-biased pulsatile chromatin opening and epigenetic regulation in adult mouse liver

   This article has 3 authors:

   1. Andy Rampersaud
   2. Jeannette Connerney
   3. David J Waxman

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study offers new and convincing support for the idea that about a third of mouse liver DNAse hypersensitivity sites (DHS) showing male-biased chromatin opening are sex-biased because of the male-specific cyclic action of growth hormone pulses to alter chromatin accessibility, as compared to the relative ineffectiveness of the more static pattern of growth hormone secretion in females. Supporting evidence is found in the impact of hypophysectomy and growth hormone treatment on chromatin accessibility, and the binding of specific transcription factors and epigenetic marks at STAT5-sensitive sites. This work uncovers mechanisms underlying sex differences in liver function and will be of broad interest to endocrinologists and hepatologists.

   Reviewed by eLife

3. Hyperactivity of mTORC1- and mTORC2-dependent signaling mediates epilepsy downstream of somatic PTEN loss

   This article has 4 authors:

   1. Erin R Cullen
   2. Mona Safari
   3. Isabelle Mittelstadt
   4. Matthew C Weston

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study investigated the role of specific proteins in a mouse model of developmental epilepsy. The significance of the work is important because a new mouse model was used to simulate a type of developmental epilepsy. The work is also significant because the deletion of two proteins together, but not separately, improved the symptoms, and data were convincing.

   Reviewed by eLife

4. Stimulation-induced cytokine polyfunctionality as a dynamic concept

   This article has 3 authors:

   1. Kevin Portmann
   2. Aline Linder
   3. Klaus Eyer

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This useful study uses a microfluidic method to evaluate the ability of single human white blood cells to produce combinations of cytokines and the evidence that this takes place is solid. The paper highlights polyfunctionality using data that are similar to a prior dataset from the same group. The authors comment that, in analysis of larger panels, single cells rarely make more than 2 or 3 cytokines so that investigation of 3 cytokines at a time is sufficient to investigate this phenomenon. Coupling this approach to other modes of single cell analysis may provide greater insight into what limits simultaneous production of multiple cytokines.

   Reviewed by eLife

5. Body size as a metric for the affordable world

   This article has 4 authors:

   1. Xinran Feng
   2. Shan Xu
   3. Yuannan Li
   4. Jia Liu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This paper presents valuable findings that shed light on the mental organisation of knowledge about real-world objects. It provides diverse, if incomplete and tentative, evidence from behaviour, brain, and large language models that this knowledge is divided categorically between relatively small objects (closer to the relevant scale for direct manipulation) and larger objects (further from the typical scope of human affordances for action).

   Reviewed by eLife

6. Involvement of superior colliculus in complex figure detection of mice

   This article has 10 authors:

   1. J Leonie Cazemier
   2. Robin Haak
   3. TK Loan Tran
   4. Ann TY Hsu
   5. Medina Husic
   6. Brandon D Peri
   7. Lisa Kirchberger
   8. Matthew W Self
   9. Pieter Roelfsema
   10. J Alexander Heimel

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The authors present a valuable work suggesting that the superficial, retinorecipient layers of the mouse superior colliculus (SC) may participate in figure-ground segregation and object recognition. These data are based largely on optogenetic perturbations of SC but the strength of evidence is currently incomplete: although the effects are statistically significant, there are significant technical limitations that are not adequately addressed via controls.

   Reviewed by eLife

7. Resting-state alterations in behavioral variant frontotemporal dementia are related to the distribution of monoamine and GABA neurotransmitter systems

   This article has 16 authors:

   1. Lisa Hahn
   2. Simon B Eickhoff
   3. Karsten Mueller
   4. Leonhard Schilbach
   5. Henryk Barthel
   6. Klaus Fassbender
   7. Klaus Fliessbach
   8. Johannes Kornhuber
   9. Johannes Prudlo
   10. Matthis Synofzik
   11. Jens Wiltfang
   12. Janine Diehl-Schmid
   13. FTLD Consortium
   14. Markus Otto
   15. Juergen Dukart
   16. Matthias L Schroeter

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study presents important findings linking structural and functional changes in frontotemporal dementia to underlying neurotransmitter systems. The evidence to support the claims is solid, however, relationships are relatively modest and there are limitations regarding the neurotransmitter data. This study will appeal to clinicians and neuroscientists who are interested in the potential effects of certain neurotransmitter systems on clinical features of frontotemporal dementia.

   Reviewed by eLife

8. Transcriptional regulation of SARS-CoV-2 receptor ACE2 by SP1

   This article has 10 authors:

   1. Hui Han
   2. Rong-Hua Luo
   3. Xin-Yan Long
   4. Li-Qiong Wang
   5. Qian Zhu
   6. Xin-Yue Tang
   7. Rui Zhu
   8. Yi-Cheng Ma
   9. Yong-Tang Zheng
   10. Cheng-Gang Zou

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This is a valuable report that describes that ACE2 expression is upregulated by SARS-CoV-2 infection via activation of transcription factor Sp1 and inhibition of HNF4α through the PI3K/AKT pathway. Inhibition of Sp1 reduces SARS-CoV-2 infection in vitro and in an animal model. This work is solid and will be of interest to those interested in ACE2 biology and its impact in COVID-19.

   Reviewed by eLife

9. ARHGAP18-ezrin functions as an autoregulatory module for RhoA in the assembly of distinct actin-based structures

   This article has 5 authors:

   1. Andrew T Lombardo
   2. Cameron AR Mitchell
   3. Riasat Zaman
   4. David J McDermitt
   5. Anthony Bretscher

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This fundamental work substantially advances our understanding of how cells can tightly modulate small GTPase activity to build and maintain neighboring cytoskeletal structures, in this case microvilli. The evidence supporting these claims is compelling and is supported by both protein-protein interaction assays as well as cell biological studies. The work will be of interest to cell biologist studying the cytoskeleton as well as those interested in G-protein mediated regulation.

   Reviewed by eLife

10. Eye-specific differences in active zone addition during synaptic competition in the developing visual system

    This article has 3 authors:

    1. Chenghang Zhang
    2. Tarlan Vatan
    3. Colenso M Speer

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This is a valuable analysis of STORM data that characterizes the clustering of active zones in retinogeniculate terminals across ages and in the absence of retinal waves. The design makes it possible to relate fixed time point structural data to a known outcome of activity-dependent remodeling. The latest revision has tempered the causal claims made in previous versions. The result provides solid structural support for the hypotheses regarding how activity influences the clustering of these synapses.

    Reviewed by eLife