eLife

Latest preprint reviews

1. Allosteric activation of the co-receptor BAK1 by the EFR receptor kinase initiates immune signaling

   This article has 5 authors:

   1. Henning Mühlenbeck
   2. Yuko Tsutsui
   3. Mark A Lemmon
   4. Kyle W Bender
   5. Cyril Zipfel

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This manuscript reports important in vitro biochemical and in planta experiments to study the receptor activation mechanism of plant membrane receptor kinase complexes with non-catalytic intracellular kinase domains. Several lines of evidence convincingly show that one such putative pseudokinase, the immune receptor EFR achieves an active conformation following phosphorylation by a co-receptor kinase, and then in turn activates the co-receptor kinase allosterically to enable it to phosphorylate down-stream signaling components. This manuscript will be of interest to scientists focusing on cell signalling and allosteric regulation.

   Reviewed by eLife

2. BMP2 and BMP7 cooperate with H3.3K27M to promote quiescence and invasiveness in pediatric diffuse midline gliomas

   This article has 27 authors:

   1. Paul Huchede
   2. Swann Meyer
   3. Clément Berthelot
   4. Maud Hamadou
   5. Adrien Bertrand-Chapel
   6. Andria Rakotomalala
   7. Line Manceau
   8. Julia Tomine
   9. Nicolas Lespinasse
   10. Paul Lewandowski
   11. Martine Cordier-Bussat
   12. Laura Broutier
   13. Aurélie Dutour
   14. Isabelle Rochet
   15. Jean-Yves Blay
   16. Cyril Degletagne
   17. Valéry Attignon
   18. Angel Montero-Carcaboso
   19. Marion Le Grand
   20. Eddy Pasquier
   21. Alexandre Vasiljevic
   22. Pascale Gilardi-Hebenstreit
   23. Samuel Meignan
   24. Pierre Leblond
   25. Vanessa Ribes
   26. Erika Cosset
   27. Marie Castets

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This valuable study examines whether the BMP signaling pathway has a role in H3.3K27M DMG tumors, regardless of the presence of ACRVR1 activating mutations. The authors provide solid evidence that BMP2/7 synergizes with H3.3K27M to induce a transcriptomic rewiring associated with a quiescent but invasive cell state. Although this work could be further enhanced by the inclusion of additional models, the study overall points to BMP2/7 as a potential target for future therapies in this deadly cancer.

   Reviewed by eLife

3. Dynamic 1D search and processive nucleosome translocations by RSC and ISW2 chromatin remodelers

   This article has 12 authors:

   1. Jee Min Kim
   2. Claudia C Carcamo
   3. Sina Jazani
   4. Zepei Xie
   5. Xinyu A Feng
   6. Maryam Yamadi
   7. Matthew Poyton
   8. Katie L Holland
   9. Jonathan B Grimm
   10. Luke D Lavis
   11. Taekjip Ha
   12. Carl Wu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This manuscript describes fundamental single-molecule correlative force and fluorescence microscopy experiments to visualize the 1D diffusion dynamics and long-range nucleosome sliding activity of the yeast chromatin remodelers, RSC and ISW2. Compelling evidence shows that both remodelers exhibit 1D diffusion on bare DNA but utilize different mechanisms, with RSC primarily hopping and ISW2 mainly sliding on DNA. These results will be of interest to researchers working on chromatin remodeling.

   Reviewed by eLife

4. Phosphorylation, disorder, and phase separation govern the behavior of Frequency in the fungal circadian clock

   This article has 7 authors:

   1. Daniyal Tariq
   2. Nicole Maurici
   3. Bradley M Bartholomai
   4. Siddarth Chandrasekaran
   5. Jay C Dunlap
   6. Alaji Bah
   7. Brian R Crane

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This manuscript is a fundamental contribution to the understanding of the role of intrinsically disordered proteins in circadian clocks and the potential involvement of phase separation mechanisms. The authors convincingly report on the structural and biochemical aspects and the molecular interactions of the intrinsically disordered protein FRQ. The paper will be of interest to scientists focusing on circadian clock regulation, liquid-liquid phase separation, and phosphorylation.

   Reviewed by eLife

5. Concerted changes in the pediatric single-cell intestinal ecosystem before and after anti-TNF blockade

   This article has 46 authors:

   1. Hengqi Betty Zheng
   2. Benjamin A Doran
   3. Kyle Kimler
   4. Alison Yu
   5. Victor Tkachev
   6. Veronika Niederlova
   7. Kayla Cribbin
   8. Ryan Fleming
   9. Brandi Bratrude
   10. Kayla Betz
   11. Lorenzo Cagnin
   12. Connor McGuckin
   13. Paula Keskula
   14. Alexandre Albanese
   15. Maria Sacta
   16. Joshua de Sousa Casal
   17. Ruben van Esch
   18. Andrew C Kwong
   19. Conner Kummerlowe
   20. Faith Taliaferro
   21. Nathalie Fiaschi
   22. Baijun Kou
   23. Sandra Coetzee
   24. Sumreen Jalal
   25. Yoko Yabe
   26. Michael Dobosz
   27. Matthew F Wipperman
   28. Sara Hamon
   29. George D Kalliolias
   30. Andrea Hooper
   31. Wei Keat Lim
   32. Sokol Haxhinasto
   33. Yi Wei
   34. Madeline Ford
   35. Lusine Ambartsumyan
   36. David L Suskind
   37. Dale Lee
   38. Gail Deutsch
   39. Xuemei Deng
   40. Lauren V Collen
   41. Vanessa Mitsialis
   42. Scott B Snapper
   43. Ghassan Wahbeh
   44. Alex K Shalek
   45. Jose Ordovas-Montanes
   46. Leslie S Kean

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study substantially advances our understanding of pediatric Crohn's disease, mapping the cellular make-up of this disease and how patients respond to treatment. The evidence supporting the conclusions is compelling, with thorough bioinformatic analyses, underpinned by rigorous methodology and data integration. The work will be of broad interest to pediatric clinicians, immunologists and bioinformaticians.

   Reviewed by eLife

6. Pesticide-induced resurgence in brown planthoppers is mediated by action on a suite of genes that promote juvenile hormone biosynthesis and female fecundity

   This article has 8 authors:

   1. Yang Gao
   2. Shao-Cong Su
   3. Ji-Yang Xing
   4. Zhao-Yu Liu
   5. Dick R Nässel
   6. Chris Bass
   7. Congfen Gao
   8. Shun-Fan Wu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This useful manuscript reports mechanisms behind the increase in fecundity in response to sub-lethal doses of pesticides in the crop pest, the brown plant hopper. The authors hypothesize that the pesticide works by inducing the JH titer, which through the JH signaling pathway induces egg development, for which the evidence was judged to be solid.

   Reviewed by eLife

7. Mapping the architecture of the initiating phosphoglycosyl transferase from S. enterica O-antigen biosynthesis in a liponanoparticle

   This article has 6 authors:

   1. Greg J Dodge
   2. Alyssa J Anderson
   3. Yi He
   4. Weijing Liu
   5. Rosa Viner
   6. Barbara Imperiali

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment:

     This valuable manuscript provides solid methodologies for utilizing SMALP nanodisks for oligomer characterization. The authors present a platform for capturing and studying native membrane protein oligomerization and subsequent cryoEM analysis. The specific application of the method to WbaP, a membrane-bound phosphoglycosyl transferase, adds to our understanding of glycoconjugate production in bacteria. This manuscript would be of interest to those focusing on native membrane protein studies and antimicrobial resistance.

   Reviewed by eLife

8. Differential regulation by CD47 and thrombospondin-1 of extramedullary erythropoiesis in mouse spleen

   This article has 5 authors:

   1. Rajdeep Banerjee
   2. Thomas J Meyer
   3. Margaret C Cam
   4. Sukhbir Kaur
   5. David D Roberts

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study presents a valuable finding on the cell composition in mouse spleen depleted for the CD47 receptor and its signaling ligand Thrombospondin in hematopoietic differentiation. The supporting evidence is convincing with analytical improvements on the individual contributions of the signaling components and with functional studies. This work has implications for the role of CD47/Thsp in extramedullary erythropoiesis in mouse spleen and will be of interest to medical biologists working on cell signaling, transfusion medicine, and cell therapy.

   Reviewed by eLife

9. Maturation and detoxification of synphilin-1 inclusion bodies regulated by sphingolipids

   This article has 10 authors:

   1. Xiuling Cao
   2. Xiang Wu
   3. Lei Zhao
   4. Ju Zheng
   5. Xuejiao Jin
   6. Xinxin Hao
   7. Joris Winderickx
   8. Shenkui Liu
   9. Lihua Chen
   10. Beidong Liu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     By combining Synthetic Genetic Array (SGA) analysis with state-of-the-art imaging techniques, this study provides strong evidence that sphingolipid metabolism controls the maturation of Parkinson's disease-associated Synphilin-1 inclusion bodies (SY1 IBs) on the mitochondrial surface in a yeast model. The authors present compelling proof that perturbing the sphingolipid metabolic pathway leads to delayed SY1 IB maturation and enhanced SY1-triggered toxicity. Altogether, the authors show the important role of sphingolipid metabolism in the detoxification process of misfolded proteins by facilitating large IB formation on the mitochondrial outer membrane.

   Reviewed by eLife

10. Catalytic growth in a shared enzyme pool ensures robust control of centrosome size

    This article has 2 authors:

    1. Deb Sankar Banerjee
    2. Shiladitya Banerjee

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This valuable work suggests a new physical model of centrosome maturation: a catalytic growth model with a shared enzyme pool. The authors provide compelling evidence to show that the model is able to reproduce various experimental results such as centrosome size scaling with cell size and centrosome growth curves in C. elegans, and that the final centrosome size is more robust to differences in initial centrosome size. While direct experimental support for this theory is currently lacking, the authors propose concrete experiments that could distinguish their shared-enzyme model from previously proposed alternatives.

    Reviewed by eLife