eLife

Latest preprint reviews

1. A translation proofreader of archaeal origin imparts multi-aldehyde stress tolerance to land plants

   This article has 12 authors:

   1. Pradeep Kumar
   2. Ankit Roy
   3. Shivapura Jagadeesha Mukul
   4. Avinash Kumar Singh
   5. Dipesh Kumar Singh
   6. Aswan Nalli
   7. Pujaita Banerjee
   8. Kandhalu Sagadevan Dinesh Babu
   9. Bakthisaran Raman
   10. Shobha P Kruparani
   11. Imran Siddiqi
   12. Rajan Sankaranarayanan

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The work is a fundamental contribution towards understanding the role of archaeal and plant D-aminoacyl-tRNA deacylase 2 (DTD2) in deacylation and detoxification of D-Tyr-tRNATyr modified by various aldehydes produced as metabolic byproducts in plants. It integrates convincing results from both in vitro and in vivo experiments to address the long-standing puzzle of why plants outperform bacteria in handling reactive aldehydes and suggests a new strategy for stress-tolerant crops. A limitation of the study is the lack of evidence for accumulation of toxic D-aminoacyl tRNAs and impairment of translation in plant cells lacking DTD2.

   Reviewed by eLife

2. Structural dynamics of the active HER4 and HER2/HER4 complexes is finely tuned by different growth factors and glycosylation

   This article has 5 authors:

   1. Raphael Trenker
   2. Devan Diwanji
   3. Tanner Bingham
   4. Kliment A Verba
   5. Natalia Jura

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This manuscript describes structures of HER4 homo- and HER4/HER2 hetero-dimer complexes using single particle cryo-EM. This important work describes convincingly new structural details of these complexes that expand our understanding of their function. This work will be of interest to researchers working on cell surface signalling and kinase activity.

   Reviewed by eLife

3. Wide transition-state ensemble as key component for enzyme catalysis

   This article has 6 authors:

   1. Gabriel E Jara
   2. Francesco Pontiggia
   3. Renee Otten
   4. Roman V Agafonov
   5. Marcelo A Martí
   6. Dorothee Kern

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     In this potentially important study, the authors report results of QM/MM simulations and kinetic measurements for the phosphoryl-transfer step in adenylate kinase. The results point to the mechanistic proposal that the transition state ensemble is broader in the most efficient form of the enzyme (i.e., in the presence of Mg2+ in the active site) and thus a different activation entropy. With a broad set of computations and experimental analyses, the level of evidence is considered solid by some reviewers. On the other hand, there remain limitations in the computational analyses, especially regarding free energy profiles using different methodologies (shape of free energy profiles with DFTB vs. PBE QM/MM, and barriers with steered MD and umbrella sampling) and the activation entropy, leading some reviewers to the evaluation that the level of evidence is incomplete.

   Reviewed by eLife

4. A novel imaging method (FIM-ID) reveals that myofibrillogenesis plays a major role in the mechanically induced growth of skeletal muscle

   This article has 11 authors:

   1. Kent W Jorgenson
   2. Jamie E Hibbert
   3. Ramy KA Sayed
   4. Anthony N Lange
   5. Joshua S Godwin
   6. Paulo HC Mesquita
   7. Bradley A Ruple
   8. Mason C McIntosh
   9. Andreas N Kavazis
   10. Michael D Roberts
   11. Troy A Hornberger

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The work by Hornberger and team presents a novel workflow for the visualisation of myofibrils with high resolution and contrast that will be highly valued by the scientific community. The methods include solid validation of both sample preparation and analysis, and have been used to make the fundamental discovery of myofibrillogenesis as the mechanism of mechanical loading-induced growth. Whether this mechanism is present in other settings of muscle growth (i.e., non-loading), other striated tissue (e.g myocardium), or is sex-dependent, will require future experiments.

   Reviewed by eLife

5. Optogenetic control of a GEF of RhoA uncovers a signaling switch from retraction to protrusion

   This article has 4 authors:

   1. Jean de Seze
   2. Maud Bongaerts
   3. Benoit Boulevard
   4. Mathieu Coppey

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study combines compelling experiments with optogenetic actuation and convincing theory to understand how signalling proteins control the switch between cell protrusion and retraction, two essential processes in single cell migration. The authors examine the importance of the basal concentration and recruitment dynamics of a guanine exchange factor (GEF) on the activity of the downstream effectors RhoA and Cdc42, which control retraction and protrusion. The experimental and theoretical evidence provides a model of RhoA's involvement in both protrusion and retraction and shows that these complex processes are highly dependent on the concentration and activity dynamics of the components.

   Reviewed by eLife

6. A 2-hydroxybutyrate-mediated feedback loop regulates muscular fatigue

   This article has 14 authors:

   1. Brennan J Wadsworth
   2. Marina Leiwe
   3. Eleanor A Minogue
   4. Pedro P Cunha
   5. Viktor Engman
   6. Carolin Brombach
   7. Christos Asvestis
   8. Shiv K Sah-Teli
   9. Emilia Marklund
   10. Peppi Koivunen
   11. Jorge L Ruas
   12. Helene Rundqvist
   13. Johanna T Lanner
   14. Randall S Johnson

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The work by Johnson and co-workers has identified an important role of 2-Hydroxybutyrate in skeletal muscle oxidative capacity in the early stages of exercise. Mechanistically, they show convincing data to support a role of 2-Hydroxybutyrate in the regulation of BCAA metabolism via SIRT4, ADP-Ribosylation, and CEBP. However, whether this is the sole mechanism and if these translate to longer exercise training regimes requires future experiments.

   Reviewed by eLife

7. Structure-guided mutagenesis of OSCAs reveals differential activation to mechanical stimuli

   This article has 4 authors:

   1. Sebastian Jojoa-Cruz
   2. Adrienne E Dubin
   3. Wen-Hsin Lee
   4. Andrew B Ward

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The manuscript seeks to dissect the molecular underpinnings of poke and stretch activation in OSCA channels. While the structural and functional experiments are well done, and the authors present some important data, the reviewers identified weaknesses in experimental design and interpretation that render the data incomplete in supporting some of the main conclusions of the paper. Nevertheless, this work will be of interest to those working in the fields of mechanosensation, sensory biology, and ion channels.

   Reviewed by eLife

8. Genetic associations between circulating immune cells and periodontitis highlight the prospect of systemic immunoregulation in periodontal care

   This article has 12 authors:

   1. Xinjian Ye
   2. Yijing Bai
   3. Mengjun Li
   4. Yuhang Ye
   5. Yitong Chen
   6. Bin Liu
   7. Yuwei Dai
   8. Shan Wang
   9. Weiyi Pan
   10. Zhiyong Wang
   11. Yingying Mao
   12. Qianming Chen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     In this fundamental study, the authors analyzed associations between circulating immune cells and periodontitis. Convincing evidence identifies three immune cell types related to periodontitis, which substantially advances our understanding of periodontitis.

   Reviewed by eLife

9. GABRD promotes the progression of breast cancer through CDK1-dependent cell cycle regulation

   This article has 12 authors:

   1. Qingyao Shang
   2. Fei Ren
   3. Kexin Feng
   4. Chenxuan Yang
   5. Shuangtao Zhao
   6. Jiaxiang Liu
   7. Xiyu Kang
   8. Jiaxian Yue
   9. Ruixuan Zhang
   10. Xiangzhi Meng
   11. Xiang Wang
   12. Xin Wang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study presents a useful finding on the role of GABRD and its downstream target CDK1 in the progression of breast cancer. The evidence supporting the claims of the authors is somewhat incomplete and the elaboration of the mechanistic details on GABARD/CDK1 regulation would have strengthened the study. The work will be of interest to clinicians and biologists working on breast cancer.

   Reviewed by eLife

10. Rapid translocation of NGR proteins driving polarization of PIN-activating D6 protein kinase during root gravitropism

    This article has 5 authors:

    1. Ivan Kulich
    2. Julia Schmid
    3. Anastasia Teplova
    4. Linlin Qi
    5. Jiří Friml

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      This fundamental study addresses the earliest events that enable plant roots to reorient growth in response to gravity. Compelling molecular and cell biological data establish that plasma membrane localization of the LAZY or NEGATIVE GRAVITROPIC RESPONSE OF ROOTS (NGR) protein family is required for rapid and polar redirection of D6 protein kinase, an activator of the PIN3 auxin transporter. This work complements and extends recent publications on the NGR family in gravity sensing (PMID: 37741279 and PMID: 37561884). Collectively these papers advance our understanding of rapid plant gravity sensing and response.

    Reviewed by eLife