eLife

Latest preprint reviews

1. A Germinal Center Checkpoint of AIRE in B Cells Limits Antibody Diversification

   This article has 25 authors:

   1. Jordan Z Zhou
   2. Bihui Huang
   3. Bo Pei
   4. Guang Wen Sun
   5. Michael D Pawlitz
   6. Wei Zhang
   7. Xinyang Li
   8. Kati C Hokynar
   9. Fayi Yao
   10. Madusha LW Perera
   11. Shanqiao Wei
   12. Simin Zheng
   13. Lisa A Polin
   14. Janet M Poulik
   15. Annamari Ranki
   16. Kai Krohn
   17. Charlotte Cunningham-Rundles
   18. Naibo Yang
   19. Ashok S Bhagwat
   20. Kefei Yu
   21. Pärt Peterson
   22. Kai Kisand
   23. Bao Q Vuong
   24. Andrea Cerutti
   25. Kang Chen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     AIRE has been well known to contribute to immune self-tolerance in the thymus by expressing auto-antigens; in this manuscript, the authors describe unexpected findings about the interaction of AIRE with AID in B cells, and its function in the immune system, thereby contributing to a fundamental understanding of the broader functions of AIRE. The strength of this manuscript is that, by employing biochemical and genetic experiments, the authors convincingly show interaction between AIRE and AID and subsequent AIRE's function in the GC responses. However, two weak points exist: first, the connection between AIRE, auto-anti IL17 Abs, and IL17-positive effector T cells, and second, like the thymus, expression of auto-antigens by AIRE in the GC B cells has not been tested.

   Reviewed by eLife

2. Structural elucidation of the hexameric MmpS4-MmpL4 complex from Mycobacterium tuberculosis

   This article has 6 authors:

   1. Jennifer C Earp
   2. Nicolas P Lichti
   3. Alisa A Garaeva
   4. Virginia Meikle
   5. Michael Niederweis
   6. Markus A Seeger

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides valuable insights through the elucidation of the first full-length structure of the heterohexameric (MmpS4)₃-(MmpL4)₃ transporter complex from Mycobacterium tuberculosis, advancing understanding of its transport mechanism, linked to virulence and drug resistance. The structural analysis is convincing, offering a clear framework for future mechanistic studies. Major strengths include a comprehensive structural characterization of the complex, though some conclusions require further validation.

   Reviewed by eLife

3. White Matter Stratification in Depression Predicts Multidimensional Antidepressant Responses

   This article has 11 authors:

   1. Jiaolong Qin
   2. Xinyi Wang
   3. Huangjing Ni
   4. Ye Wu
   5. Haiyan Liu
   6. Lingling Hua
   7. Rui Yan
   8. Hao Tang
   9. Peng Zhao
   10. Zhijian Yao
   11. Qing Lu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents valuable findings for identifying biotypes of depression patients using white matter measures, which are under-utilised and under-appreciated in current biological and computational psychiatry work. The evidence supporting the claims is solid, although enhanced interpretability of the identified biotypes across both white matter and symptom levels, and better justification of the choice of models would strengthen the paper. Overall, this study will be of interest to the broad community of neuroimagers, clinicians, and biological and computational psychiatry researchers.

   Reviewed by eLife

4. A systematic interactome of SET1C expands its functional landscape and identifies candidate regulatory connections

   This article has 15 authors:

   1. Pierre Luciano
   2. Kihyun Park
   3. Stéphane Audebert
   4. Luc Camoin
   5. Carlos A Niño
   6. Da Kyeong Park
   7. Isabella E Maudlin
   8. Marion Dubarry
   9. Lara Lee
   10. Marlene Oeffinger
   11. Jean D Beggs
   12. Young Hye Kim
   13. Jaehoon Kim
   14. Bernhard Dichtl
   15. Vincent Géli

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study uses the yeast two-hybrid assay to identify proteins that may interact with yeast Set1 and other subunits of COMPASS/Set1C, the histone H3K4 methyltransferase, providing also some evidence for Set1 sumoylation and a role of SET1C methylating other factors in vitro. The results are valuable and they should contribute to understanding the functions of the conserved SET1C complex, as they suggest potential functional connections with RNA biogenesis, chromatin remodeling, and non-histone methylation whose implications would yet need to be explored. Nevertheless, apart from the fact that only a small subset of the Y2H interactions is further examined, the validating experiments are only partial or inconclusive, the strength of evidence being incomplete at this point, although with improvements over the previous version.

   Reviewed by eLife

5. Proteomic composition and mutual assembly of the C2a projection in vertebrate motile cilia

   This article has 11 authors:

   1. Qian Lyu
   2. Qingchao Li
   3. Jingrui Li
   4. Jiajun Luo
   5. Chunyu Liu
   6. Shanshan Nai
   7. Hongbin Liu
   8. Xueliang Zhu
   9. Ting Song
   10. Min Liu
   11. Huijie Zhao

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides valuable insights into the protein composition of the C2a projection in mouse motile cilia, building upon prior work in Chlamydomonas. The evidence supporting the claims of the authors is solid. The work will be of interest to biologists and clinicians studying cilia and ciliopathies.

   Reviewed by eLife

6. Primordial cardiomyocytes orchestrate myocardial morphogenesis and vascularization but are dispensable for regeneration

   This article has 3 authors:

   1. Jisheng Sun
   2. Lu Chen
   3. Jinhu Wang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important work identifies phlda2 as a specific marker for primordial cardiomyocytes in the adult zebrafish heart and demonstrates their essential role in myocardial morphogenesis and coronary vascularization, but not in heart regeneration. The conclusions are well supported by single-cell transcriptomics, new genetic tools, and cell-specific ablation experiments. Overall, the evidence is solid and provides insight into the difference between developmental and regenerative cardiac programs. This work will be of interest for those studying cardiac development and regeneration.

   Reviewed by eLife

7. Alternative splicing of PIF4 regulates plant development under heat stress

   This article has 6 authors:

   1. María Niño-González
   2. Benjamin Alary
   3. Dóra Szakonyi
   4. Tom Laloum
   5. Paula Duque
   6. Guiomar Martín

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This manuscript identifies temperature-dependent alternative splicing of PIF4 in Arabidopsis thaliana and shows that heat stress promotes the accumulation of a short exon 5-skipping isoform that is predicted to encode a non-functional protein. This finding is important, and it provides an intriguing new layer of regulation for PIF4; however, the strength of the mechanistic conclusions is limited, and several key conclusions rely on indirect evidence. As a result, while the data robustly demonstrate heat-regulated alternative splicing of PIF4, the causal role of PIF4 isoforms' balance in shaping heat-induced developmental responses remains only partially supported and the strength of the evidence presented is incomplete. This work will be of interest to biologists working on alternative splicing.

   Reviewed by eLife

8. Deep mutational scanning reveals pharmacologically relevant insights into TYK2 signaling and disease

   This article has 24 authors:

   1. Conor J Howard
   2. Nathan S Abell
   3. Robert R Warneford-Thomson
   4. Eden Mahdavi
   5. Alan L Su
   6. Carmen Resnick
   7. Nabil Mohammed
   8. Erin M Thompson
   9. Emily R Holzinger
   10. Katrina Catalano
   11. Abhay Hukku
   12. Gabriel A Mintier
   13. Morgan MacKenzie
   14. Bryan L Jiang
   15. Dora Barbosa Rabago
   16. Angela Chan
   17. Carolindah Ntimi
   18. Kaitlyn N Weiler
   19. Stephen C Wilson
   20. Joseph C Maranville
   21. Payal R Sheth
   22. Robert M Plenge
   23. Sriram Kosuri
   24. Diane E Dickel

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important paper presents a rigorous and comprehensive deep mutational analysis of the kinase TYK2, revealing how single amino acid substitutions influence protein abundance, signaling activity, and responses to pharmacological inhibitors. By combining high‑quality experimental design with dose‑response signaling assays and multiple inhibitor conditions, the authors generate a robust dataset that identifies variants across all domains of TYK2, including clusters at functionally critical sites and protein-protein interfaces. The study highlights mutations that drive drug resistance or potentiation and shows that reduced TYK2 abundance aligns with protective autoimmune‑associated variants, underscoring the therapeutic relevance of modulating TYK2 stability. Overall, the work provides compelling insights with clear implications for biochemistry, immunology, clinical genetics, and drug discovery.

   Reviewed by eLife

9. Flexible and high-throughput simultaneous profiling of gene expression and chromatin accessibility in single cells

   This article has 5 authors:

   1. Volker Soltys
   2. Moritz Peters
   3. Dingwen Su
   4. Marek Kučka
   5. Yingguang Frank Chan

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents a useful methodological advance that better enables the simultaneous measurement of gene expression and chromatin accessibility in individual cells. The evidence supporting the improved detection of gene expression is solid. The method has the potential to be more broadly impactful if it were expanded to include orthogonal validation strategies. This method will be of interest to those studying transcription and gene regulation.

   Reviewed by eLife

10. Temporal single-cell analysis reveals age-associated delay in immune resolution after respiratory viral infection

    This article has 17 authors:

    1. Yue Wu
    2. Chaofan Li
    3. Jinyi Tang
    4. Xiaochen Gao
    5. In Su Cheon
    6. Bibo Zhu
    7. Ruixuan Zhang
    8. Cori E Fain
    9. Shengen Shawn Hu
    10. Harish Narasimhan
    11. Gislane de Almeida Santos
    12. Katayoun Ayasoufi
    13. Aaron J Johnson
    14. Hui Zong
    15. Chongzhi Zang
    16. Haidong Dong
    17. Jie Sun

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This study presents valuable findings that could potentially allow a deeper understanding of the immunopathogenesis underlying influenza infection in aged mice. The results are based on solid evidence that define putative immune determinants underlying immunopathology in the aged lung. This study will be of interest to researchers pursuing aging research, as well as to immunologists.

    Reviewed by eLife