eLife Assessment
In this study, the authors found that a species of aphid that is a known agricultural pest salivated longer and produced more honeydew when feeding at night. The authors identified aphid genes with diurnal expression patterns, including potential salivation-related genes. Silencing these genes reduced aphid performance only on plants and not on artificial diet, suggesting a specific role in plant feeding. This study is valuable for understanding plant-insect interactions in agriculture and presents solid evidence of diurnal rhythmicity in aphid activity and performance, although further research is needed to elucidate the function of the identified genes.