eLife

Latest preprint reviews

1. The nanoscale organization of the Nipah virus fusion protein informs new membrane fusion mechanisms

   This article has 7 authors:

   1. Qian Wang
   2. Jinxin Liu
   3. Yuhang Luo
   4. Vicky Kliemke
   5. Giuliana Leonarda Matta
   6. Jingjing Wang
   7. Qian Liu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study advances our understanding of how Nipah virus fusion protein F (NiV-F) organizes into nanoclusters on cell and viral membranes using biochemical and super-resolution microscopy methods. The conclusions are supported by solid evidence and the revision has addressed most of the reviewers' concerns. The relationship between clustering and fusion is of high interest and an interesting hypothesis to continue investigating in future studies.

   Reviewed by eLife

2. Capsaicin acts as a novel NRF2 agonist to suppress ethanol induced gastric mucosa oxidative damage by directly disrupting the KEAP1-NRF2 interaction

   This article has 21 authors:

   1. Xiaoning Gao
   2. WuYan Guo
   3. Peiyuan Liu
   4. Mingyue Yuwen
   5. Hongyu Ren
   6. Shengtao Hu
   7. Zixiang Liu
   8. Ruyang Tan
   9. Kairui Liu
   10. Zhiru Yang
   11. Junli Ba
   12. Xue Bai
   13. Shiti Shama
   14. Cong Tang
   15. Kai Miao
   16. Haozhi Pei
   17. Liren Liu
   18. Cheng Zhu
   19. Tao Wang
   20. Bo Zhang
   21. Jun Kang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study suggests that capsaicin nanoparticle administration in rats activates the transcription factor Nrf2 by directly binding to its repressor, KEAP1, leading to the induction of cytoprotective genes and preventing alcohol-induced gastric damage, offering a potential avenue for treating alcoholism-related gastric disorders. The authors provide solid evidence through a wealth of biochemical experiments in vitro, in cultured cells as well as in a rat model. The work will be of great interest to researchers studying oxidative damage in a variety of different diseases and the exploitation of molecules for therapeutic approaches.

   Reviewed by eLife

3. JAK-STAT pathway activation compromises nephrocyte function in a Drosophila high-fat diet model of chronic kidney disease

   This article has 5 authors:

   1. Yunpo Zhao
   2. Jianli Duan
   3. Hannah Seah
   4. Joyce van de Leemput
   5. Zhe Han

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents important new insights linking obesity to kidney disease using a Drosophila model. A series of compelling experiments demonstrate that a high-fat diet induces excretion of a leptin-like JAK-STAT ligand from fat body, driving the adipose-nephrocyte axis through activated JAK-STAT signaling and subsequently causing a functional defect in nephrocytes. The approach using combination of genetic tools and pharmacological intervention is solid and confirms the mechanistic link, together with phenotypic analysis that further supports the authors conclusions.

   Reviewed by eLife

4. TMEM16 and OSCA/TMEM63 proteins share a conserved potential to permeate ions and phospholipids

   This article has 7 authors:

   1. Augustus J Lowry
   2. Pengfei Liang
   3. Mo Song
   4. Yuichun Wan
   5. Zhen-Ming Pei
   6. Huanghe Yang
   7. Yang Zhang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study advances our understanding of the mechanisms controlling lipid flux and ion permeation in the TMEM16 and OSCA/TMEM63 family channels. The study provides compelling new evidence indicating that side chains along the TM4/6 interface play a key role in gating lipid and ion fluxes in these channels. The authors suggest that the transmembrane channel/scramblase family proteins may have originally functioned as scramblases but lost this capacity over evolution.

   Reviewed by eLife

5. The actomyosin system is essential for the integrity of the endosomal system in bloodstream form Trypanosoma brucei

   This article has 14 authors:

   1. Fabian Link
   2. Sisco Jung
   3. Xenia Malzer
   4. Felix Zierhut
   5. Antonia Konle
   6. Alyssa Borges
   7. Christopher Batters
   8. Monika Weiland
   9. Mara Poellmann
   10. An Binh Nguyen
   11. Johannes Kullmann
   12. Claudia Veigel
   13. Markus Engstler
   14. Brooke Morriswood

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study builds on a previous publication, demonstrating that T. brucei has a continuous endomembrane system, which probably facilitates high rates of endocytosis. Using a range of cutting-edge approaches, the authors present compelling evidence that an actomyosin system, with the myosin TbMyo1 as an active molecular motor, is localized close to and can associate with the endosomal system in the bloodstream form of Trypanosoma brucei. It shows convincingly that both actin and Myo I play a role in the organization and integrity of the endosomal system: both RNAi-mediated depletion of Myo1, and treatment of the cells with latrunculin A resulted in endomembrane disruption. This work should be of interest to cell biologists and microbiologists working on the cytoskeleton, and unicellular eukaryotes.

   Reviewed by eLife, Arcadia Science

6. Dynamic chromatin architecture identifies new autoimmune-associated enhancers for IL2 and novel genes regulating CD4+ T cell activation

   This article has 12 authors:

   1. Matthew C Pahl
   2. Prabhat Sharma
   3. Rajan M Thomas
   4. Zachary Thompson
   5. Zachary Mount
   6. James A Pippin
   7. Peter A Morawski
   8. Peng Sun
   9. Chun Su
   10. Daniel Campbell
   11. Struan FA Grant
   12. Andrew D Wells

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This is a solid study that follows a well-established canvas for variant-to-gene prioritisation using 3D genomics, applying it to activated T cells. The authors go some way in validating the lists of candidate genes, as well as exploring the regulatory architecture of a candidate GWAS locus. Jointly with data from previous studies performing variant-to-gene assignment in activated CD4 T cells (and other immune cells), this work provides a useful additional resource for interpreting autoimmune disease-associated genetic variation.

   Reviewed by eLife

7. Additional feedforward mechanism of Parkin activation via binding of phospho-UBL and RING0 in trans

   This article has 7 authors:

   1. Dipti Ranjan Lenka
   2. Shakti Virendra Dahe
   3. Odetta Antico
   4. Pritiranjan Sahoo
   5. Alan R Prescott
   6. Miratul MK Muqit
   7. Atul Kumar

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This is a useful manuscript describing the competitive binding between Parkin domains to define the importance of dimerization in the mechanism of Parkin regulation and catalytic activity. The evidence supporting the importance of Parkin dimerization for an 'in trans' model of Parkin activity described in this manuscript is solid, but lacks more stringent and biochemical characterization of competitive binding that could provide more direct evidence to support the author's conclusions. This work will be of interest to those focused on defining the molecular mechanisms involved in ubiquitin ligase interactions, PINK-Parkin-mediated mitophagy, and mitochondrial organellar quality control.

   Reviewed by eLife

8. A-to-I RNA editing of CYP18A1 mediates transgenerational wing dimorphism in aphids

   This article has 6 authors:

   1. Bin Zhu
   2. Rui Wei
   3. Wenjuan Hua
   4. Lu Li
   5. Wenlin Zhang
   6. Pei Liang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents an important finding on the molecular mechanism for transduction of environmentally induced polyphenism. The evidence supporting the claims of the author is solid. This paper would be of interest to those studying aphids wing dimorphism.

   Reviewed by eLife

9. Endogenous oligomer formation underlies DVL2 condensates and promotes Wnt/β-catenin signaling

   This article has 7 authors:

   1. Senem Ntourmas
   2. Martin Sachs
   3. Petra Paclíková
   4. Martina Brückner
   5. Vítězslav Bryja
   6. Jürgen Behrens
   7. Dominic B Bernkopf

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study contributes to the understanding of phase separation in Dishevelled (DVL) proteins, by investigating the endogenous complexes of DVL2 using ultracentrifugation and contrasting them with DVL1 and DVL3 behaviour and the functional validation of the DVL2 intrinsically disordered regions mediating the protein condensate. The study includes a solid characterisation of several overexpression constructs, including in KO cells. However, investigations of the roles of the described DVL2 regions at the endogenous level remain to be carried out.

   Reviewed by eLife

10. Knockout of cyclin-dependent kinases 8 and 19 leads to depletion of cyclin C and suppresses spermatogenesis and male fertility in mice

    This article has 23 authors:

    1. Alexandra V Bruter
    2. Ekaterina A Varlamova
    3. Nina I Stavskaya
    4. Zoia G Antysheva
    5. Vasily N Manskikh
    6. Anna V Tvorogova
    7. Diana S Korshunova
    8. Alvina I Khamidullina
    9. Marina V Utkina
    10. Viktor P Bogdanov
    11. Iuliia P Baikova
    12. Alyona I Nikiforova
    13. Eugene A Albert
    14. Denis O Maksimov
    15. Jing Li
    16. Mengqian Chen
    17. Gary P Schools
    18. Alexey V Feoktistov
    19. Alexander A Shtil
    20. Igor B Roninson
    21. Vladislav A Mogila
    22. Yulia Y Silaeva
    23. Victor V Tatarskiy

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This valuable study reports the critical role of two cyclin-dependent kinases, CDK8 and CDK19, in spermatogenesis. The data presented are generally supportive of the main conclusion and are considered solid. This work may be of interest to reproductive biologists and physicians working on male fertility.

    Reviewed by eLife