eLife

Latest preprint reviews

1. Diverging roles of TRPV1 and TRPM2 in warm-temperature detection

   This article has 4 authors:

   1. Muad Y Abd El Hay
   2. Gretel B Kamm
   3. Alejandro Tlaie Boria
   4. Jan Siemens

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     In this manuscript, Abd El Hay and colleagues use an innovative behavioral assay and analysis method, together with standard calcium imaging experiments on cultured dorsal root ganglion (DRG) neurons, to evaluate the consequences of global knockout of TRPV1 and TRPM2, and overexpression of TRPV1, on warmth detection. Compelling evidence is provided for a role of TRPM2 channels in warmth avoidance behavior, but it remains unclear whether this involves channel activity in the periphery or in the brain. In contrast, TRPV1 is clearly implicated at the cellular level in warmth detection. These findings are important because there is substantial ongoing discussion regarding the contribution of TRP channels to different aspects of thermo-sensation.

   Reviewed by eLife

2. Lateral/caudal ganglionic eminence makes limited contribution to cortical oligodendrocytes

   This article has 10 authors:

   1. Jialin Li
   2. Feihong Yang
   3. Yu Tian
   4. Ziwu Wang
   5. Dashi Qi
   6. Zhengang Yang
   7. Jiangang Song
   8. Jing Ding
   9. Xin Wang
   10. Zhuangzhi Zhang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The authors provide solid evidence that any contribution of oligodendrocyte precursors to the developing cortex from the lateral ganglionic eminence is minimal in scope. The methods used support the conclusions, with some technical concerns that the authors can address with further experimentation. These are considered valuable additions to our understanding of the origins of oligodendrocytes in the forebrain during development.

   Reviewed by eLife

3. Neutralizing gut-derived lipopolysaccharide as a novel therapeutic strategy for severe leptospirosis

   This article has 6 authors:

   1. Xufeng Xie
   2. Xi Chen
   3. Shilei Zhang
   4. Jiuxi Liu
   5. Wenlong Zhang
   6. Yongguo Cao

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The gut microbiota influences many infectious diseases; however, its role Leptospirosis remains unclear. In this fundamental work, Xie et al. use a hamster model to show that Leptospira infection leads to gut pathology, an altered gut microbiota, and increased translocation. A combined use of antibiotics and LPS neutralization prolonged survival, providing a potential new therapeutic approach. This study utilizes compelling methods to provide new insights into this emerging disease, which could be dissected further in future studies aimed at gaining mechanistic insight and assessing the translational relevance of these discoveries.

   Reviewed by eLife

4. Distinct T-cell receptor (TCR) gene segment usage and MHC-restriction between foetal and adult thymus

   This article has 7 authors:

   1. Jasmine Rowell
   2. Ching-In Lau
   3. Susan Ross
   4. Diana C Yanez
   5. Oscar A Peña
   6. Benny Chain
   7. Tessa Crompton

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important manuscript provides an extensive and convincing analysis of the foetal and adult TCR repertoire in the mouse thymus. A potential implication of the work is that the earliest appearing T cells during ontogeny may have properties that are fundamentally distinct from those appearing later in life. The study will be of interest to immunologists concerned with T cell development and TCR repertoires.

   Reviewed by eLife

5. Convergent epigenetic evolution drives relapse in acute myeloid leukemia

   This article has 8 authors:

   1. Kevin Nuno
   2. Armon Azizi
   3. Thomas Koehnke
   4. Caleb Lareau
   5. Asiri Ediriwickrema
   6. M Ryan Corces
   7. Ansuman T Satpathy
   8. Ravindra Majeti

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important work substantially advances our understanding of how resistant leukemia can arise without changes in mutational patterns by displaying epigenetic changes. The evidence supporting the conclusions is compelling, with rigorous genomic assays done on primary samples. and state-of-the-art microscopy. The work will be of broad interest to hematologists and cancer biologists.

   Reviewed by eLife

6. Tissue-specific O-GlcNAcylation profiling identifies substrates in translational machinery in Drosophila mushroom body contributing to olfactory learning

   This article has 13 authors:

   1. Haibin Yu
   2. Dandan Liu
   3. Yaowen Zhang
   4. Ruijun Tang
   5. Xunan Fan
   6. Song Mao
   7. Lu Lv
   8. Fang Chen
   9. Hongtao Qin
   10. Zhuohua Zhang
   11. Daan MF van Aalten
   12. Bing Yang
   13. Kai Yuan

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This work describes a valuable new technique involving proximity labelling to identify Drosophila proteins modified by GlcNAcylation in subsets of cells in vivo. A solid set of experiments shows that several ribosomal proteins are modified in the fly mushroom body. Consistent with a role for GlcNAcylation of ribosomal proteins in control of memory related translational control, the authors show that perturbation of GlcNAc modification in KCs prevents efficient consolidation of long-term memory.

   Reviewed by eLife

7. A novel mouse model for LAMA2-related muscular dystrophy with analysis of molecular pathogenesis and clinical phenotype

   This article has 10 authors:

   1. Dandan Tan
   2. Yidan Liu
   3. Huaxia Luo
   4. Qiang Shen
   5. Xingbo Long
   6. Luzheng Xu
   7. Jieyu Liu
   8. Nanbert A Zhong
   9. Hong Zhang
   10. Hui Xiong

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This useful manuscript reports on a new mouse model for LAMA2-MD, a rare but very severe congenital muscular dystrophy. The knockout mice were generated by removing exon3 in the Lama2 gene, which results in a frameshift in exon4 and a premature stop codon. These animals lack any laminin-alpha2 protein and confirm results from previous Lama2 knockout models. Additionally, this study includes weak transcriptomics data that might be a good resource for the field. However, experimental evidence, methods, and data analyses supporting the main claims of the manuscript are incomplete.

   Reviewed by eLife

8. Excitatory neurons in stratum radiatum provide an alternative pathway for excitation flow that escapes perisomatic inhibition

   This article has 10 authors:

   1. Julia Lebedeva
   2. David Jappy
   3. Azat Nasretdinov
   4. Alina Vazetdinova
   5. Viktoria Krut
   6. Rostislav Sokolov
   7. Yulia Dobryakova
   8. Marina Eliava
   9. Valery Grinevich
   10. Andrei Rozov

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This work addresses the connectivity of giant excitatory neurons in a part of CA1 of the hippocampus. Recordings in rat brain slices provide new evidence that these cells excite bistratified and basket inhibitory neurons, and have weak inhibitory input from basket cells, as well as other findings. This circuitry gives these cells unique potential, making the work valuable, however the strength of the evidence is currently incomplete.

   Reviewed by eLife

9. Secreted antigen A peptidoglycan hydrolase is essential for Enterococcus faecium cell separation and priming of immune checkpoint inhibitor therapy

   This article has 9 authors:

   1. Steven Klupt
   2. Kyong Tkhe Fam
   3. Xing Zhang
   4. Pavan Kumar Chodisetti
   5. Abeera Mehmood
   6. Tumara Boyd
   7. Danielle Grotjahn
   8. Donghyun Park
   9. Howard C Hang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The authors build upon prior data implicating the secreted peptidoglycan hydrolase SagA produced by Enterococcus faecium in immunotherapy. Leveraging new strains with sagA deletion/complementation constructs, the investigators reveal that sagA is non-essential, with sagA deletion leading to a marked growth defect due to impaired cell division, and sagA being necessary for the immunogenic and anti-tumor effects of E. faecium. In aggregate, the study utilizes compelling methods to provide both fundamental new insights into E. faecium biology and host interactions and a proof-of-concept for identifying the bacterial effectors of immunotherapy response.

   Reviewed by eLife

10. The genetic architecture of the load linked to dominant and recessive self-incompatibility alleles in Arabidopsis halleri and Arabidopsis lyrata

    This article has 8 authors:

    1. Audrey Le Veve
    2. Mathieu Genete
    3. Christelle Lepers-Blassiau
    4. Chloé Ponitzki
    5. Céline Poux
    6. Xavier Vekemans
    7. Eleonore Durand
    8. Vincent Castric

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      This study presents valuable empirical work and simulations that are relevant for the evolution of genetic load linked to self-incompatibility alleles in two Arabidopsis species. The evidence supporting the findings is solid, although it remains to be seen how generalizable the conclusions are beyond the specific system investigated here, not least because the statistical significance varied between the two species. The work will be of relevance to geneticists interested in the evolution of allelic diversity in similar systems.

    Reviewed by eLife