eLife

Latest preprint reviews

1. Mechanism of dimer selectivity and binding cooperativity of BRAF inhibitors

   This article has 6 authors:

   1. Joseph Clayton
   2. Aarion Romany
   3. Evangelia Matenoglou
   4. Evripidis Gavathiotis
   5. Poulikos I Poulikakos
   6. Jana Shen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This fundamental study illuminates the dynamics of BRAF in its monomeric and dimeric forms, both in the absence and presence of inhibitors, through a convincing combination of traditional experiments and sophisticated computational analyses. By revealing novel insights into the selectivity and cooperative processes of BRAF inhibitors, it holds significant promise for the development of future therapeutics, particularly against mutant isoforms in cancer. Overall, these findings will be of great interest to structural biologists, medicinal chemists, and pharmacologists.

   Reviewed by eLife

2. Context dependent contributions of the direct and indirect pathways in the associative and sensorimotor striatum

   This article has 6 authors:

   1. Nisa Cuevas
   2. Argelia Llanos-Moreno
   3. Kathia I. Ramírez-Armenta
   4. Hector Alatriste-León
   5. Josué O. Ramírez Jarquin
   6. Fatuel Tecuapetla

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This valuable manuscript shows that the optogenetic stimulation of direct and indirect pathway spiny projection neurons (SPNs) in the dorsomedial versus the dorsolateral striatum has different consequences for locomotor activity, real-time place preference, and action selection, in a contextually mediated manner. The evidence in support of this conclusion is solid but would be further strengthened through deeper analysis of the effect and specificity of optogenetic manipulations on SPN activity. These findings will be of interest to neuroscientists, particularly behavioral neuroscientists.

   Reviewed by eLife

3. Multimodal mismatch responses in mouse auditory cortex

   This article has 2 authors:

   1. Magdalena Solyga
   2. Georg B Keller

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This well-designed study provides important findings concerning the way the brain encodes prediction about self-generated sensory inputs. The authors report that neurons in auditory cortex respond to mismatches in locomotion-driven auditory feedback and that those responses can be enhanced by concurrent mismatches in visual inputs. While there remain alternative explanations for some of the data, these findings provide convincing support for the role of predictive processing in cortical function by indicating that sensorimotor prediction errors in one modality influence the computation of prediction errors in another modality.

   Reviewed by eLife

4. Neural activity ramps in frontal cortex signal extended motivation during learning

   This article has 7 authors:

   1. Josue M Regalado
   2. Ariadna Corredera Asensio
   3. Theresa Haunold
   4. Andrew C Toader
   5. Yan Ran Li
   6. Lauren A Neal
   7. Priyamvada Rajasethupathy

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important manuscript provides compelling experimental evidence of extended motivational signals encoded in the mouse anterior cingulate cortex (ACC) that are implemented by orbitofrontal cortex (OFC)-to-ACC signaling during learning. The experimental methods used were state-of-the-art. These results will be of interest to those interested in cortical function, learning, and/or motivation.

   Reviewed by eLife

5. Brain-derived and in vitro-seeded alpha-synuclein fibrils exhibit distinct biophysical profiles

   This article has 3 authors:

   1. Selene Seoyun Lee
   2. Livia Civitelli
   3. Laura Parkkinen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important work compares the strain properties of a-synuclein fibrils isolated from LBD and MSA patient samples with the resulting amplified fibrils following SAA. Using orthogonal biochemical and structural approaches to strengthen their analyses, the authors provide solid evidence that the SAA-amplified fibrils do not recapitulate the disease-relevant strains present in the patient samples. CryoEM would further strengthen this data but it is outside the scope of the work. This work should be considered in the widespread applications of SAA in synucleopathies and its potential limitations.

   Reviewed by eLife

6. Longitudinal awake imaging of mouse deep brain microvasculature with super-resolution ultrasound localization microscopy

   This article has 7 authors:

   1. Yike Wang
   2. Matthew R Lowerison
   3. Zhe Huang
   4. Qi You
   5. Bing-Ze Lin
   6. Daniel A Llano
   7. Pengfei Song

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents important methodologies for repeated brain ultrasound localization microscopy (ULM) in awake mice and a set of results indicating that wakefulness reduces vascularity and blood flow velocity. The data supporting these findings are solid. This study is relevant for scientists investigating vascular physiology in the brain.

   Reviewed by eLife

7. Refining the resolution of the yeast genotype–phenotype map using single-cell RNA-sequencing

   This article has 4 authors:

   1. Arnaud N'Guessan
   2. Wen Yuan Tong
   3. Hamed Heydari
   4. Alex N Nguyen Ba

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This useful study describes expression profiling by scRNA-seq of thousands of cells of recombinant yeast genotypes from a system that models natural genetic variation. The rigorous new method presented here shows promise for improving the efficiency of genotype-to-phenotype mapping in yeast, providing convincing evidence for its efficacy. This manuscript focuses on overcoming technical challenges with this approach and identifies several new biological insights that build upon the field of genotype-to-phenotype mapping, a central question of interest to geneticists and evolutionary biologists.

   Reviewed by eLife

8. A microRNA that controls the emergence of embryonic movement

   This article has 4 authors:

   1. Jonathan AC Menzies
   2. André Maia Chagas
   3. Tom Baden
   4. Claudio R Alonso

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study presents a new quantitative imaging pipeline that describes with high temporal precision and throughput the movements of late-stage Drosophila embryos, a critical moment when motion first appears. A new approach is used to explore the role of miRNAs in motion onset and presents solid evidence that shows a role for miR-2b-1 and its target Motor in embryonic motion. The data are well supported even if the mechanistic insight into the emergence of movement remains to be explored.

   Reviewed by eLife

9. Actin dynamics switches two distinct modes of endosomal fusion in yolk sac visceral endoderm cells

   This article has 7 authors:

   1. Seiichi Koike
   2. Masashi Tachikawa
   3. Motosuke Tsutsumi
   4. Takuya Okada
   5. Tomomi Nemoto
   6. Kazuko Keino-Masu
   7. Masayuki Masu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study provides valuable insights into the role of actin dynamics in regulating the transition of fusion models during homotypic fusion between late endosomes. The evidence supporting the authors' claims is convincing. However, while the observations are significant, the study could benefit from further exploration of the mechanistic details and physiological relevance.

   Reviewed by eLife

10. The title and authors for this article are not available from our external data provider yet:
    10.48550/arxiv.2305.18417v3