eLife

Latest preprint reviews

1. Pial collaterals develop through mosaic colonization of capillaries by arterial and microvascular endothelial cells

   This article has 7 authors:

   1. Tijana Perovic
   2. Irene Hollfinger
   3. Stefanie Mayer
   4. Janet Lips
   5. Monika Dopatka
   6. Christoph Harms
   7. Holger Gerhardt

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study provides insights into the developmental origin of endothelial cells found in blood vessels called pial collaterals. The work is important, as collateral capacity can strongly influence the trajectory of outcomes with vascular blockage, and the approaches are novel and overall convincing; however, some mechanistic claims are only partially supported, and collateral characterization is incomplete. Given the clear positive correlation between pial collateral flow and improved stroke outcome, this study will be of interest to vascular biologists and clinicians caring for stroke patients.

   Reviewed by eLife

2. The molecular logic of Gtr1/2- and Pib2-dependent TORC1 regulation in budding yeast

   This article has 6 authors:

   1. Jacob H Cecil
   2. Cristina M Padilla
   3. Austin A Lipinski
   4. Paul Langlais
   5. Xiangxia Luo
   6. Andrew P Capaldi

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The study presents valuable findings concerning how a highly conserved signal transduction pathway helps budding yeast cells adapt their growth to nitrogen sources of differing qualities. However, the evidence is incomplete for the authors' main claim that the pathway adopts three distinct states depending on the nitrogen source. The presented data, particularly phospho-proteomic datasets, will be of interest to the cell growth signaling community.

   Reviewed by eLife

3. Stem-loop and circle-loop TADs generated by directional pairing of boundary elements have distinct physical and regulatory properties

   This article has 4 authors:

   1. Wenfan Ke
   2. Miki Fujioka
   3. Paul Schedl
   4. James B Jaynes

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This valuable work investigates the role of boundary elements in the formation of 3D genome architecture. The authors established a specific model system that allowed them to manipulate boundary elements and examine the resulting genome topology. The work yielded the first demonstration of the existence of stem and circle loops in a genome and confirms a model which had been posited based on extensive prior genetic work, providing insights into how 3D genome topologies affect enhancer-promoter communication. The evidence is solid, although the degree of generalization remains uncertain.

   Reviewed by eLife

4. Inhibition of the UFD-1-NPL-4 complex triggers an aberrant immune response in Caenorhabditis elegans

   This article has 3 authors:

   1. Rajneesh Rao
   2. Alejandro Aballay
   3. Jogender Singh

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     In this valuable manuscript, Rao and colleagues investigate the UFD-1/NPL-4 complex, which is involved in extracting misfolded proteins in the plasma membrane and the accumulation of pathogenic bacteria in the intestine. Using convincing methods, the authors find that knockdown of the ufd-1 and npl-4 genes leads to shortened lifespan of the nematode C. elegans and reduced accumulation of the bacterial pathogen P. aeruginosa in the intestine.

   Reviewed by eLife

5. Chromosome structure in Drosophila is determined by boundary pairing not loop extrusion

   This article has 8 authors:

   1. Xinyang Bing
   2. Wenfan Ke
   3. Miki Fujioka
   4. Amina Kurbidaeva
   5. Sarah Levitt
   6. Mike Levine
   7. Paul Schedl
   8. James B Jaynes

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This valuable work presents elegant experimental data from the Drosophila embryo supporting the notion that interactions among specific loci, called boundary elements, contribute to topologically associated domain (TAD) formation and gene regulation. The evidence supporting boundary:boundary pairing as a determinant of 3D structures is compelling; however, an inability to deplete loop extruders formally leaves open a possible contribution of loop extrusion. This study will be of interest to the nuclear structure community, particularly those using Drosophila as a model.

   Reviewed by eLife

6. Structure of scavenger receptor SCARF1 and its interaction with lipoproteins

   This article has 14 authors:

   1. Yuanyuan Wang
   2. Fan Xu
   3. Guangyi Li
   4. Chen Cheng
   5. Bowen Yu
   6. Ze Zhang
   7. Dandan Kong
   8. Fabao Chen
   9. Yali Liu
   10. Zhen Fang
   11. Longxing Cao
   12. Yang Yu
   13. Yijun Gu
   14. Yongning He

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     SCARF1 is a scavenger membrane-bound receptor that binds modified versions of lipoproteins and has a significant role in maintaining lipid homeostasis. This useful study reports the crystal structure of SCARF1 and identifies putative binding sites for modified lipoproteins. Supported by a convincing set of experimental approaches, this study advances our knowledge of how scavenger receptors clear modified lipoproteins to maintain lipid homeostasis.

   Reviewed by eLife

7. The title and authors for this article are not available from our external data provider yet:
   10.48550/arxiv.2301.00759v2

8. Hemodynamics regulate spatiotemporal artery muscularization in the developing circle of Willis

   This article has 6 authors:

   1. Siyuan Cheng
   2. Ivan Fan Xia
   3. Renate Wanner
   4. Javier Abello
   5. Amber N Stratman
   6. Stefania Nicoli

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study provides the first analysis of vascular stabilization on the critical and evolutionarily conserved structure around the Circle of Willis in the brain, strengthened by using parallel in vivo and in vitro experimental approaches. The evidence supporting the claims is solid and the work will be valuable for scientists studying developmental and disease-related vascular stabilization.

   Reviewed by eLife

9. The non-mitotic role of HMMR in regulating the localization of TPX2 and the dynamics of microtubules in neurons

   This article has 4 authors:

   1. Yi-Ju Chen
   2. Shun-Cheng Tseng
   3. Peng-Tzu Chen
   4. Eric Hwang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     In their valuable study, Chen et al. investigate the neuronal role of HMMR, a microtubule-associated protein typically associated with cell division. Their findings indicate that HMMR is necessary for proper neuronal morphology and the generation of polymerizing microtubules within neurites, potentially by promoting the function of TPX2. This solid body of work is the first step in deciphering the influence of a mitotic microtubule-associated protein in organizing microtubules in neurons and will be of interest to the neurobiology and cytoskeleton fields.

   Reviewed by eLife

10. Rtf1-dependent transcriptional pausing regulates cardiogenesis

    This article has 11 authors:

    1. Adam D. Langenbacher
    2. Fei Lu
    3. Luna Tsang
    4. Zi Yi Stephanie Huang
    5. Benjamin Keer
    6. Zhiyu Tian
    7. Alette Eide
    8. Matteo Pellegrini
    9. Haruko Nakano
    10. Atsushi Nakano
    11. Jau-Nian Chen

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      This important study conducts genetic analyses utilizing zebrafish, mouse, and mouse embryonic stem cell models to elucidate the role of Rtf1, a component of the PAF1 complex, in early cardiac development. By combining marker gene expression analysis, single-cell transcriptomics, ChIP-seq, and chemical inhibition, the study provides convincing evidence that Rtf1-mediated RNAPII (Pol2) transcriptional pausing is required for early cardiac development and that attenuation of pause release by pharmacological inhibition of Cdk9, a component of the PTEF-b complex that regulates the transition between the pausing and elongation phases of transcription, can partially restore transcriptional pausing and cardiogenesis in zebrafish rtf1 mutants. The work will be of broad interest to developmental biologists.

    Reviewed by eLife