eLife

Latest preprint reviews

1. The actomyosin system is essential for the integrity of the endosomal system in bloodstream form Trypanosoma brucei

   This article has 14 authors:

   1. Fabian Link
   2. Sisco Jung
   3. Xenia Malzer
   4. Felix Zierhut
   5. Antonia Konle
   6. Alyssa Borges
   7. Christopher Batters
   8. Monika Weiland
   9. Mara Poellmann
   10. An Binh Nguyen
   11. Johannes Kullmann
   12. Claudia Veigel
   13. Markus Engstler
   14. Brooke Morriswood

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study builds on a previous publication, demonstrating that T. brucei has a continuous endomembrane system, which probably facilitates high rates of endocytosis. Using a range of cutting-edge approaches, the authors present compelling evidence that an actomyosin system, with the myosin TbMyo1 as an active molecular motor, is localized close to and can associate with the endosomal system in the bloodstream form of Trypanosoma brucei. It shows convincingly that both actin and Myo I play a role in the organization and integrity of the endosomal system: both RNAi-mediated depletion of Myo1, and treatment of the cells with latrunculin A resulted in endomembrane disruption. This work should be of interest to cell biologists and microbiologists working on the cytoskeleton, and unicellular eukaryotes.

   Reviewed by eLife, Arcadia Science

2. Dynamic chromatin architecture identifies new autoimmune-associated enhancers for IL2 and novel genes regulating CD4+ T cell activation

   This article has 12 authors:

   1. Matthew C Pahl
   2. Prabhat Sharma
   3. Rajan M Thomas
   4. Zachary Thompson
   5. Zachary Mount
   6. James A Pippin
   7. Peter A Morawski
   8. Peng Sun
   9. Chun Su
   10. Daniel Campbell
   11. Struan FA Grant
   12. Andrew D Wells

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This is a solid study that follows a well-established canvas for variant-to-gene prioritisation using 3D genomics, applying it to activated T cells. The authors go some way in validating the lists of candidate genes, as well as exploring the regulatory architecture of a candidate GWAS locus. Jointly with data from previous studies performing variant-to-gene assignment in activated CD4 T cells (and other immune cells), this work provides a useful additional resource for interpreting autoimmune disease-associated genetic variation.

   Reviewed by eLife

3. Additional feedforward mechanism of Parkin activation via binding of phospho-UBL and RING0 in trans

   This article has 7 authors:

   1. Dipti Ranjan Lenka
   2. Shakti Virendra Dahe
   3. Odetta Antico
   4. Pritiranjan Sahoo
   5. Alan R Prescott
   6. Miratul MK Muqit
   7. Atul Kumar

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This is a useful manuscript describing the competitive binding between Parkin domains to define the importance of dimerization in the mechanism of Parkin regulation and catalytic activity. The evidence supporting the importance of Parkin dimerization for an 'in trans' model of Parkin activity described in this manuscript is solid, but lacks more stringent and biochemical characterization of competitive binding that could provide more direct evidence to support the author's conclusions. This work will be of interest to those focused on defining the molecular mechanisms involved in ubiquitin ligase interactions, PINK-Parkin-mediated mitophagy, and mitochondrial organellar quality control.

   Reviewed by eLife

4. A-to-I RNA editing of CYP18A1 mediates transgenerational wing dimorphism in aphids

   This article has 6 authors:

   1. Bin Zhu
   2. Rui Wei
   3. Wenjuan Hua
   4. Lu Li
   5. Wenlin Zhang
   6. Pei Liang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents an important finding on the molecular mechanism for transduction of environmentally induced polyphenism. The evidence supporting the claims of the author is solid. This paper would be of interest to those studying aphids wing dimorphism.

   Reviewed by eLife

5. Endogenous oligomer formation underlies DVL2 condensates and promotes Wnt/β-catenin signaling

   This article has 7 authors:

   1. Senem Ntourmas
   2. Martin Sachs
   3. Petra Paclíková
   4. Martina Brückner
   5. Vítězslav Bryja
   6. Jürgen Behrens
   7. Dominic B Bernkopf

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study contributes to the understanding of phase separation in Dishevelled (DVL) proteins, by investigating the endogenous complexes of DVL2 using ultracentrifugation and contrasting them with DVL1 and DVL3 behaviour and the functional validation of the DVL2 intrinsically disordered regions mediating the protein condensate. The study includes a solid characterisation of several overexpression constructs, including in KO cells. However, investigations of the roles of the described DVL2 regions at the endogenous level remain to be carried out.

   Reviewed by eLife

6. Knockout of cyclin-dependent kinases 8 and 19 leads to depletion of cyclin C and suppresses spermatogenesis and male fertility in mice

   This article has 23 authors:

   1. Alexandra V Bruter
   2. Ekaterina A Varlamova
   3. Nina I Stavskaya
   4. Zoia G Antysheva
   5. Vasily N Manskikh
   6. Anna V Tvorogova
   7. Diana S Korshunova
   8. Alvina I Khamidullina
   9. Marina V Utkina
   10. Viktor P Bogdanov
   11. Iuliia P Baikova
   12. Alyona I Nikiforova
   13. Eugene A Albert
   14. Denis O Maksimov
   15. Jing Li
   16. Mengqian Chen
   17. Gary P Schools
   18. Alexey V Feoktistov
   19. Alexander A Shtil
   20. Igor B Roninson
   21. Vladislav A Mogila
   22. Yulia Y Silaeva
   23. Victor V Tatarskiy

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study reports the critical role of two cyclin-dependent kinases, CDK8 and CDK19, in spermatogenesis. The data presented are generally supportive of the main conclusion and are considered solid. This work may be of interest to reproductive biologists and physicians working on male fertility.

   Reviewed by eLife

7. The interplay between biomolecular assembly and phase separation

   This article has 4 authors:

   1. Giacomo Bartolucci
   2. Ivar S Haugerud
   3. Thomas CT Michaels
   4. Christoph A Weber

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The authors present an important theoretical framework that describes the interplay between liquid-liquid phase separation and protein aggregation within a mean-field model. This work will be of high interest to the biophysics and molecular biology communities, as it will help understand and analyse assembly within biomolecular condensates in cells or in-vitro. Major strengths of this convincing work are the consideration of aggregates with various dimensionality and the possibility for protein gelation.

   Reviewed by eLife

8. CTFFIND5 provides improved insight into quality, tilt, and thickness of TEM samples

   This article has 4 authors:

   1. Johannes Elferich
   2. Lingli Kong
   3. Ximena Zottig
   4. Nikolaus Grigorieff

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable work presents the latest version of CTFFIND, which is the most popular software for determination of the contrast transfer function (CTF) in cryo-electron microscopy. CTFFIND5 estimates and considers acquisition geometry and sample thickness, which leads to improved CTF determination. The paper describes compelling evidence that CTFFIND5 finds better CTF parameters than previous methods, in particular for tilted samples (e.g. for cryo-electron tomography) or where thickness is an issue (e.g. cellular samples, or electron microscopy at low voltages).

   Reviewed by eLife

9. CXCR3-expressing myeloid cells recruited to the hypothalamus protect against diet-induced body mass gain and metabolic dysfunction

   This article has 10 authors:

   1. Natalia Mendes
   2. Ariane Zanesco
   3. Cristhiane Aguiar
   4. Gabriela F Rodrigues-Luiz
   5. Dayana Silva
   6. Jonathan Campos
   7. Niels Olsen Saraiva Camara
   8. Pedro Moraes-Vieira
   9. Eliana Araujo
   10. Licio A Velloso

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This work is of fundamental significance and has an exceptional level of evidence for a new population that protects against obesity-induced hypothalamic inflammation. This topic will attract attention from a broad base of readers, from hypothalamic neuroscientists to immunologists with an interest in metabolism.

   Reviewed by eLife

10. Using normative models pre-trained on cross-sectional data to evaluate intra-individual longitudinal changes in neuroimaging data

    This article has 8 authors:

    1. Barbora Rehak Buckova
    2. Charlotte Fraza
    3. Rastislav Rehák
    4. Marián Kolenič
    5. Christian F Beckmann
    6. Filip Španiel
    7. Andre F Marquand
    8. Jaroslav Hlinka

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This paper addresses an important topic (normative trajectory modelling), seeking to provide a method aiming to accurately reflect the individual deviation of longitudinal/temporal change compared to the normal temporal change characterized based on a pre-trained population normative model. The evidence provided for the new methods is solid.

    Reviewed by eLife