eLife

Latest preprint reviews

1. An Hfq-dependent post-transcriptional mechanism fine tunes RecB expression in Escherichia coli

   This article has 6 authors:

   1. Irina Kalita
   2. Ira Alexandra Iosub
   3. Lorna McLaren
   4. Louise Goossens
   5. Sander Granneman
   6. Meriem El Karoui

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     Combining experimental and computation approaches, this manuscript provides convincing evidence for a post-transcriptional mechanism that provides robust control over the protein expression level of RecB in E. coli. In addition to uncovering how DNA damage drives higher levels of RecB protein, this work also reveals important tenets for how broader mechanisms that suppress noise and underlie responsive tuning of protein levels can be achieved.

   Reviewed by eLife

2. LncRNA Snhg3 aggravates hepatic steatosis via PPARγ signaling

   This article has 23 authors:

   1. Xianghong Xie
   2. Mingyue Gao
   3. Wei Zhao
   4. Chunmei Li
   5. Weihong Zhang
   6. Jiahui Yang
   7. Yinliang Zhang
   8. Enhui Chen
   9. Yanfang Guo
   10. Zeyu Guo
   11. Minglong Zhang
   12. Ebenezeri Erasto Ngowi
   13. Heping Wang
   14. Xiaoman Wang
   15. Yinghan Zhu
   16. Yiting Wang
   17. Xiaolu Li
   18. Hong Yao
   19. Li Yan
   20. Fude Fang
   21. Meixia Li
   22. Aijun Qiao
   23. Xiaojun Liu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study provides useful evidence substantiating a role for long noncoding RNAs in liver metabolism and organismal physiology. Using murine knockout and knock-in models, the authors invoke a previously unidentified role for the lncRNA Snhg3 in fatty liver. The revised manuscript has improved and most studies are backed by solid evidence but the study was found to be incomplete and will require future studies to substantiate some of the claims.

   Reviewed by eLife

3. Astrogliosis and neuroinflammation underlie scoliosis upon cilia dysfunction

   This article has 17 authors:

   1. Morgane Djebar
   2. Isabelle Anselme
   3. Guillaume Pezeron
   4. Pierre-Luc Bardet
   5. Yasmine Cantaut-Belarif
   6. Alexis Eschstruth
   7. Diego López-Santos
   8. Hélène Le Ribeuz
   9. Arnim Jenett
   10. Hanane Khoury
   11. Joelle Veziers
   12. Caroline Parmentier
   13. Aurélie Hirschler
   14. Christine Carapito
   15. Ruxandra Bachmann-Gagescu
   16. Sylvie Schneider-Maunoury
   17. Christine Vesque

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This valuable study analyzes the role of rpgrip1l encoding a ciliary transition zone component in the development of neuroinflammation and scoliotic phenotypes in zebrafish. Through proteomic and experimental validation in vivo, the authors demonstrated increased Annexin A2 expression and astrogliosis in the brains of scoliosis fish. Anti-inflammatory drug treatment restored normal spine development in these mutant fish, thus providing additional convincing evidence for the role of neuroinflammation in the development of scoliosis in zebrafish.

   Reviewed by eLife

4. Clearance of protein aggregates during cell division

   This article has 7 authors:

   1. Shoukang Du
   2. Yuhan Wang
   3. Bowen Chen
   4. Shuangshuang Xie
   5. Kuan Yoow Chan
   6. David C Hay
   7. Ting Gang Chew

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     How misfolded proteins are segregated and cleared is a significant question in cell biology, since clearance of these aggregates can protect against pathologies that may otherwise arise. The authors discover a cell cycle stage-dependent clearing mechanism that involves the ER chaperone BiP, the proteosome, and CDK inactivation, but is curiously independent of the anaphase promoting complex (APC). These are valuable and interesting new observations, and the evidence supporting these claims is solid.

   Reviewed by eLife

5. Microtubule-dependent orchestration of centriole amplification in brain multiciliated cells

   This article has 13 authors:

   1. Amélie-Rose Boudjema
   2. Rémi Balagué
   3. Cayla E Jewett
   4. Gina M LoMastro
   5. Olivier Mercey
   6. Adel Al Jord
   7. Marion Faucourt
   8. Alexandre Schaeffer
   9. Camille Noûs
   10. Nathalie Delgehyr
   11. Andrew J Holland
   12. Nathalie Spassky
   13. Alice Meunier

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     In this important study, Boudjema et al. use cell culture models and advanced microscopic imaging to provide detailed analyses of the cellular events underlying centriole amplification, apical migration, and assembly of hundreds of motile cilia in multi-ciliated cells. This largely descriptive work provides a better understanding of this process that is of interest to cell biologists studying centrioles and cilia. Most of the claims are supported by the data, but the study would benefit from additional analyses regarding the roles of microtubules, which are currently incomplete, and from text editing to improve accessibility and readability, especially for a wider audience.

   Reviewed by eLife

6. Superoxide Dismutases maintain niche homeostasis in stem cell populations

   This article has 4 authors:

   1. Olivia Majhi
   2. Aishwarya Chhatre
   3. Tanvi Chaudhary
   4. Devanjan Sinha

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     In this work, the authors intend to assess the existence of a redox potential across germline stem cells and neighbouring somatic stem cells in the Drosophila testis. Some aspects of the manuscript are convincing, like the clear effect of SOD KD on cyst cell differentiation state. Other conclusions of the work, such as the non-autonomous effect of this KD on germ cells are not sufficiently supported by the data. This remains true even with the revised version of the paper, as the effect of redox state of the soma on the germline is a major point of the paper, and this remains a critical flaw. The work could be potentially useful if the critiques of the reviewers were fully addressed; the strength of the evidence of the manuscript as it stands is still inadequate. Readers should use their own judgment about the validity and meaningfulness of different findings.

   Reviewed by eLife

7. The title and authors for this article are not available from our external data provider yet:
   10.48550/arxiv.2307.05304v2

8. Glycan-shielded homodimer structure and dynamical features of the canine distemper virus hemagglutinin relevant for viral entry and efficient vaccination

   This article has 19 authors:

   1. Hideo Fukuhara
   2. Kohei Yumoto
   3. Miyuki Sako
   4. Mizuho Kajikawa
   5. Toyoyuki Ose
   6. Mihiro Kawamura
   7. Mei Yoda
   8. Surui Chen
   9. Yuri Ito
   10. Shin Takeda
   11. Mwila Mwaba
   12. Jiaqi Wang
   13. Takao Hashiguchi
   14. Jun Kamishikiryo
   15. Nobuo Maita
   16. Chihiro Kitatsuji
   17. Makoto Takeda
   18. Kimiko Kuroki
   19. Katsumi Maenaka

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The manuscript presents valuable findings, using solid techniques and approaches, that shed additional light into how the canine distemper virus (CDV) hemagglutinin might engage cellular receptors and how that engagement impacts host tropism. The structural data and their analysis were thorough and well-presented. The HS-AFM data, which indicate that homodimers may dissociate into monomers - and thus have significant implications for the model of fusion triggering - are very exciting, but require further validation, perhaps by alternate approaches, to bolster the current molecular model of the CDV fusion triggering.

   Reviewed by eLife

9. Time to Eat - A Personalized Circadian Eating Schedule Leads to Weight Loss Without Imposing Calorie Restriction: A Randomized Controlled Pilot Study

   This article has 6 authors:

   1. Isabell Wilming
   2. Jana Tuschewski
   3. Jessie M Osterhaus
   4. Theresa JG Bringmann
   5. Anisja Hühne-Landgraf
   6. Dominic Landgraf

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study investigates a dietary intervention that employs a smartphone app to promote meal regularity, findings that have theoretical or practical implications for a subfield and may be clinically useful. The intervention to entice participants to adhere to specific meal times represents a restrictive diet (even though it does not ask to limit caloric intake) similar to a time-restricted feeding diet, while the control subjects are not experiencing or adhering to dietary restrictions. The authors report significant weight loss but did not rigorously assess caloric intake which remains a weakness of this study as food diaries are notoriously unreliable. While the concept is very interesting, the study is considered incomplete, and the rigor of the results should be strengthened in follow-up studies to add more stringent methods to assess caloric intake. Additionally, the study hypothesizes that the intervention resets the circadian clock. However, the study needs an objective method for assessing circadian rhythms, such as actigraphy, in addition to a subjective questionnaire.

   Reviewed by eLife

10. Compositional editing of extracellular matrices by CRISPR/Cas9 engineering of human mesenchymal stem cell lines

    This article has 13 authors:

    1. Sujeethkumar Prithiviraj
    2. Alejandro Garcia Garcia
    3. Karin Linderfalk
    4. Bai Yiguang
    5. Sonia Ferveur
    6. Ludvig Nilsén Falck
    7. Agatheeswaran Subramaniam
    8. Sofie Mohlin
    9. David Hidalgo Gil
    10. Steven J Dupard
    11. Dimitra Zacharaki
    12. Deepak Bushan Raina
    13. Paul E Bourgine

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      The study presents a potentially valuable approach to genetically modify cells to produce extracellular matrices with altered compositions, termed cell-laid, engineered extracellular matrices (eECM). The evidence supporting the authors' conclusions regarding the utility of eECM for endogenous repair is solid, although there are some disagreements on the chondrogenicity of lyophilized constructs which was viewed as lacking robust evidence for endochondral ossification.

    Reviewed by eLife