eLife

Latest preprint reviews

1. Longitudinal Awake Imaging of Mouse Deep Brain Microvasculature with Super-resolution Ultrasound Localization Microscopy

   This article has 7 authors:

   1. Yike Wang
   2. Matthew R Lowerison
   3. Zhe Huang
   4. Qi You
   5. Bing-Ze Lin
   6. Daniel A Llano
   7. Pengfei Song

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents important methodologies for repeated brain ultrasound localization microscopy (ULM) in awake mice and a set of results indicating that wakefulness reduces vascularity and blood flow velocity. The data supporting these findings are solid. This study is relevant for scientists investigating vascular physiology in the brain.

   Reviewed by eLife

2. Refining the resolution of the yeast genotype–phenotype map using single-cell RNA-sequencing

   This article has 4 authors:

   1. Arnaud N'Guessan
   2. Wen Yuan Tong
   3. Hamed Heydari
   4. Alex N Nguyen Ba

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This useful study describes expression profiling by scRNA-seq of thousands of cells of recombinant yeast genotypes from a system that models natural genetic variation. The rigorous new method presented here shows promise for improving the efficiency of genotype-to-phenotype mapping in yeast, providing convincing evidence for its efficacy. This manuscript focuses on overcoming technical challenges with this approach and identifies several new biological insights that build upon the field of genotype-to-phenotype mapping, a central question of interest to geneticists and evolutionary biologists.

   Reviewed by eLife

3. A microRNA that controls the emergence of embryonic movement

   This article has 4 authors:

   1. Jonathan AC Menzies
   2. André Maia Chagas
   3. Tom Baden
   4. Claudio R Alonso

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study presents a new quantitative imaging pipeline that describes with high temporal precision and throughput the movements of late-stage Drosophila embryos, a critical moment when motion first appears. A new approach is used to explore the role of miRNAs in motion onset and presents solid evidence that shows a role for miR-2b-1 and its target Motor in embryonic motion. The data are well supported even if the mechanistic insight into the emergence of movement remains to be explored.

   Reviewed by eLife

4. Actin dynamics switches two distinct modes of endosomal fusion in yolk sac visceral endoderm cells

   This article has 7 authors:

   1. Seiichi Koike
   2. Masashi Tachikawa
   3. Motosuke Tsutsumi
   4. Takuya Okada
   5. Tomomi Nemoto
   6. Kazuko Keino-Masu
   7. Masayuki Masu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study provides valuable insights into the role of actin dynamics in regulating the transition of fusion models during homotypic fusion between late endosomes. The evidence supporting the authors' claims is convincing. However, while the observations are significant, the study could benefit from further exploration of the mechanistic details and physiological relevance.

   Reviewed by eLife

5. The title and authors for this article are not available from our external data provider yet:
   10.48550/arxiv.2305.18417v3

6. The DBD-α4 helix of EWSR1::FLI1 is required for GGAA microsatellite binding that underlies genome regulation in Ewing sarcoma

   This article has 7 authors:

   1. Ariunaa Bayanjargal
   2. Cenny Taslim
   3. Iftekhar A Showpnil
   4. Julia Selich-Anderson
   5. Jesse C Crow
   6. Stephen L Lessnick
   7. Emily R Theisen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This paper investigates how the EWS::FLI1 fusion protein organizes chromatin topology and regulates gene expression in an aggressive pediatric bone cancer known as Ewing sarcoma. The authors used the most recent genomics methodologies to provide solid-based evidence for the role of a short alpha helix in the DNA binding domain of FLI1 in modulating binding to GGAA microsatellites and promoting enhancer activity. The study provides valuable insight into the underlying oncogenic mechanisms in Ewing sarcoma, despite the inherent limitations of the some of the techniques used.

   Reviewed by eLife

7. Structural features of heteromeric channels composed of CALHM2 and CALHM4 paralogs

   This article has 3 authors:

   1. Katarzyna Drożdżyk
   2. Martina Peter
   3. Raimund Dutzler

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     In this interesting study, Drożdżyk and colleagues analyze the ability of placental CALHM orthologs to form stable complexes, identifying that CALHM2 and CALHM4 form heterooligomeric channels. The authors then determine cryo-EM structures of heterooligomeric CALHM2 and CALHM4 that reveal a distinct arrangement in which the two orthologs can interact, but preferentially segregate in the channel. This is an important study; the data provide compelling support for the interpretations and overall, the work is clearly described.

   Reviewed by eLife

8. Loss of ZNRF3/RNF43 Unleashes EGFR in Cancer

   This article has 19 authors:

   1. Fei Yue
   2. Amy T Ku
   3. Payton D Stevens
   4. Megan N Michalski
   5. Weiyu Jiang
   6. Jianghua Tu
   7. Zhongcheng Shi
   8. Yongchao Dou
   9. Yi Wang
   10. Xin-Hua Feng
   11. Galen Hostetter
   12. Xiangwei Wu
   13. Shixia Huang
   14. Noah F Shroyer
   15. Bing Zhang
   16. Bart O Williams
   17. Qingyun Liu
   18. Xia Lin
   19. Yi Li

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This manuscript presents solid evidence suggesting that the loss of ZNRF3 and RNF43, two E3 ubiquitin ligases, leads to dysregulation of EGFR signaling in cancer. The authors propose that EGFR is a direct substrate of ZNRF3/RNF43. While the authors provide immunoprecipitation data showing increased detection of ubiquitinated species, this evidence does not definitively establish that EGFR itself is ubiquitinated by RNF43/ZNRF3. The absence of direct evidence for EGFR ubiquitination is a major limitation, although the findings are useful as they may provide novel insights into the mechanisms underlying EGFR-driven cancers and open new therapeutic avenues.

   Reviewed by eLife

9. Rab10 regulates neuropeptide release by maintaining Ca2+ homeostasis and protein synthesis

   This article has 7 authors:

   1. Jian Dong
   2. Mian Chen
   3. Jan RT van Weering
   4. Ka Wan Li
   5. August B Smit
   6. Ruud F Toonen
   7. Matthijs Verhage

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     In this revised manuscript, Dong et al. investigate the role of the small Ras-like GTPase Rab10 in the exocytosis of DCVs in mouse hippocampal neurons, showing that Rab10 depletion hinders DCV exocytosis independently of its effects on neurite outgrowth. Upon revising their work, these findings provide compelling evidence that Rab10 depletion leads to altered ER morphology, impaired ER-based calcium buffering, and decreased ribosomal protein expression, which collectively contributes to defective DCV secretion. The study comes to the fundamental conclusion that Rab10 is critical for DCV release by ensuring ER calcium homeostasis.

   Reviewed by eLife

10. A neurotransmitter atlas of C. elegans males and hermaphrodites

    This article has 10 authors:

    1. Chen Wang
    2. Berta Vidal
    3. Surojit Sural
    4. Curtis Loer
    5. G Robert Aguilar
    6. Daniel M Merritt
    7. Itai Antoine Toker
    8. Merly C Vogt
    9. Cyril C Cros
    10. Oliver Hobert

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      This fundamental study reports the most comprehensive neurotransmitter atlas of any organism to date, using fluorescent knock-in reporter lines. The work is comprehensive, rigorous, and compelling. The tool will be used by broad audience of scientists interested in neuronal cell type differentiation and function, and could be a seminal reference in the field.

    Reviewed by eLife