eLife

Latest preprint reviews

1. The rhizobial effector NopT targets Nod factor receptors to regulate symbiosis in Lotus japonicus

   This article has 12 authors:

   1. Hanbin Bao
   2. Yanan Wang
   3. Haoxing Li
   4. Qiang Wang
   5. Yutao Lei
   6. Ying Ye
   7. Syed F Wadood
   8. Hui Zhu
   9. Christian Staehelin
   10. Gary Stacey
   11. Shutong Xu
   12. Yangrong Cao

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This manuscript presents important findings on a bacterial effector involved in plant symbiotic signaling. The effector proteolytically targets a key receptor while its activity is counteracted by host-mediated phosphorylation, revealing a dynamic interplay that fine-tunes symbiotic interactions. The evidence supporting these claims is solid, and the findings have potential signaling implications beyond bacterial interactions with plants.

   Reviewed by eLife

2. The common Sting1 HAQ, AQ alleles rescue CD4 T cellpenia, restore T-regs, and prevent SAVI (N153S) inflammatory disease in mice

   This article has 7 authors:

   1. Alexandra a Aybar-Torres
   2. Lennon A Saldarriaga
   3. Ann T Pham
   4. Amir M Emtiazjoo
   5. Ashish K Sharma
   6. Andrew j Bryant
   7. Lei Jin

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study describes useful mouse models of knock-ins of human STING1 variants and an assessment of these variants' action in mouse immune cells. While the implications of the variants in the inflammatory response are of significant interest, limitations are still found in the authors' interpretation and conclusions made, and the evidence for the conclusion remains incomplete.

   Reviewed by eLife

3. Neurotrophic factor Neuritin modulates T cell electrical and metabolic state for the balance of tolerance and immunity

   This article has 17 authors:

   1. Hong Yu
   2. Hiroshi Nishio
   3. Joseph Barbi
   4. Marisa Mitchell-Flack
   5. Paolo DA Vignali
   6. Ying Zheng
   7. Andriana Lebid
   8. Kwang-Yu Chang
   9. Juan Fu
   10. Makenzie Higgins
   11. Ching-Tai Huang
   12. Xuehong Zhang
   13. Zhiguang Li
   14. Lee Blosser
   15. Ada Tam
   16. Charles Drake
   17. Drew Pardoll

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The neurotrophic factor Neuritin can moderate T-cell tolerance and immunity through both regulatory T (Treg) and effector T cells, promoting Treg cell expansion and suppression while dampening effector T cells to mediate the inflammatory response. Neuritin expression influences the membrane potential, ion channels, and nutrient transporter expression patterns of CD4+ T cells, contributing to differential metabolic states in Treg and effector T cells. These findings are solid and important for understanding immune regulation involving Treg cells and effector T cells.

   Reviewed by eLife

4. The bacterial quorum sensing signal 2’-aminoacetophenone rewires immune cell bioenergetics through the Ppargc1a/Esrra axis to mediate tolerance to infection

   This article has 8 authors:

   1. Arijit Chakraborty
   2. Arunava Bandyopadhaya
   3. Vijay K Singh
   4. Filip Kovacic
   5. Sujin Cha
   6. William M Oldham
   7. A Aria Tzika
   8. Laurence G Rahme

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study demonstrates that the Pseudomonas aeruginosa-derived quorum sensing signal, 2-aminoacetophenone, induces immune tolerization in macrophages by perturbing metabolism, particularly in the context of mitochondrial respiration and bioenergetics. The authors present convincing evidence for 2-aminoacetophenone-mediated reduction of pyruvate transport into mitochondria, with downstream effects that result in reduced ATP production in tolerized macrophages. The work will be of interest to those studying host-pathogen interactions.

   Reviewed by eLife

5. Unveiling the influence of tumor and immune signatures on immune checkpoint therapy in advanced lung cancer

   This article has 21 authors:

   1. Nayoung Kim
   2. Sehhoon Park
   3. Areum Jo
   4. Hye Hyeon Eum
   5. Hong Kwan Kim
   6. Kyungjong Lee
   7. Jong Ho Cho
   8. Bo Mi Ku
   9. Hyun Ae Jung
   10. Jong-Mu Sun
   11. Se-Hoon Lee
   12. Jin Seok Ahn
   13. Jung-Il Lee
   14. Jung Won Choi
   15. Dasom Jeong
   16. Minsu Na
   17. Huiram Kang
   18. Jeong Yeon Kim
   19. Jung Kyoon Choi
   20. Hae-Ock Lee
   21. Myung-Ju Ahn

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The authors utilized single-cell RNA-seq profiling of non-small cell lung cancer (NSCLC) patient tumor samples to generate useful insights into the determinants of immune checkpoint inhibitor (ICI) responsiveness in NSCLC patients. While some of the findings add weight to the current literature, the analysis is incomplete due to the small cohort size and heterogeneous population which has limited their ability to draw statistically supported conclusion after adjusting for multiple hypothesis testing, as well as the lack of functional characterization of the findings. This study would benefit from external cohorts to both validate the findings and justify the statistical analysis undertaken.

   Reviewed by eLife

6. TCR transgenic clone selection guided by immune receptor analysis and single-cell RNA expression of polyclonal responders

   This article has 11 authors:

   1. Nincy Debeuf
   2. Sahine Lameire
   3. Manon Vanheerswynghels
   4. Julie Deckers
   5. Caroline De Wolf
   6. Wendy Toussaint
   7. Rein Verbeke
   8. Kevin Verstaen
   9. Hamida Hammad
   10. Stijn Vanhee
   11. Bart N Lambrecht

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The paper illustrates a valuable approach to generating TCR transgenic mice specific for known epitopes. Solid evidence validates the described pipeline for identification of TCRs from single-cell datasets for the generation of TCR transgenic mice, while obviating the need for generation of T-cell lines and hybridomas.

   Reviewed by eLife

7. Identification of novel myelodysplastic syndromes prognostic subgroups by integration of inflammation, cell-type composition, and immune signatures in the bone marrow

   This article has 13 authors:

   1. Sila Gerlevik
   2. Nogayhan Seymen
   3. Shan Hama
   4. Warisha Mumtaz
   5. I Richard Thompson
   6. Seyed R Jalili
   7. Deniz E Kaya
   8. Alfredo Iacoangeli
   9. Andrea Pellagatti
   10. Jacqueline Boultwood
   11. Giorgio Napolitani
   12. Ghulam J Mufti
   13. Mohammad M Karimi

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This manuscript uses public datasets of myelodysplastic syndrome (MDS) patients to undertake a multi-omics analysis of clinical, genomic, and transcriptomic datasets. Useful findings are provided by way of interesting correlations of specific mutations with inflammation and differing clinical outcomes. The evidence is solid and interesting, and the manuscript is of substantive value to hematologists and clinical immunologists.

   Reviewed by eLife

8. Resting natural killer cells promote the progress of colon cancer liver metastasis by elevating tumor-derived stem cell factor

   This article has 16 authors:

   1. Chenchen Mao
   2. Yanyu Chen
   3. Dong Xing
   4. Teming Zhang
   5. Yangxuan Lin
   6. Cong Long
   7. Jiaye Yu
   8. Yunhui Luo
   9. Tao Ming
   10. Wangkai Xie
   11. Zheng Han
   12. Dianfeng Mei
   13. Dan Xiang
   14. Mingdong Lu
   15. Xian Shen
   16. Xiangyang Xue

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This useful study draws on published single-cell and spatial transcriptomic data of colon cancer liver metastasis to clarify the pro- and anti-tumorigenic properties of NK cells. The authors discover increased GZMK+ resting NK cells in the tumor tissue and reduced abundance of KIR2DL4+ activated NK cells. However, the evidence is currently incomplete, as the models used to validate the hypothesis and claims are not adequate and lack the necessary controls.

   Reviewed by eLife

9. The title and authors for this article are not available from our external data provider yet:
   10.48550/arxiv.2310.02257

10. PAbFold: Linear Antibody Epitope Prediction using AlphaFold2

    This article has 6 authors:

    1. Jacob DeRoo
    2. James S Terry
    3. Ning Zhao
    4. Timothy J Stasevich
    5. Christopher D Snow
    6. Brian J Geiss

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      In this manuscript, the authors describe a new AlphaFold2 pipeline called PabFold that can represent a useful tool for identifying linear antibody epitopes (B-cell epitopes) for different antigens. This information can be used in the selection of different reagents in competitive ELISA assays which can save time and reduce costs. Because several questions about the work remain, the study is currently incomplete.

    Reviewed by eLife