eLife

Latest preprint reviews

1. Thymic dendritic cell-derived IL-27p28 promotes the establishment of functional bias against IFN-γ production in newly generated CD4+ T cells through STAT1-related epigenetic mechanisms

   This article has 6 authors:

   1. Jie Zhang
   2. Hui Tang
   3. Haoming Wu
   4. Xuewen Pang
   5. Rong Jin
   6. Yu Zhang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents a useful reassessment of the potential role of dendritic cell-derived IL-27 p28 cytokine in the functional maturation of CD4+CD8- thymocytes, and CD4+ recent thymic emigrants. The evidence supporting the claims of the authors is solid and serves to reaffirm what has been previously described, with the overall advance in understanding the mechanism(s) responsible for the intrathymic functional programming of CD4+ T cells being limited.

   Reviewed by eLife

2. ATG6 interacting with NPR1 increases Arabidopsis thaliana resistance to Pst DC3000/avrRps4 by increasing its nuclear accumulation and stability

   This article has 10 authors:

   1. Baihong Zhang
   2. Shuqin Huang
   3. Shuyu Guo
   4. Yixuan Meng
   5. Yuzhen Tian
   6. Yue Zhou
   7. Hang Chen
   8. Xue Li
   9. Jun Zhou
   10. Wenli Chen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study investigates the role of ATG6 in regulating NPR1, a key protein in the plant immune response. The authors present compelling evidence that ATG6 not only interacts with NPR1 in both the cytoplasm and nucleus but also enhances its stability and nuclear accumulation, leading to increased resistance to Pst DC3000/avrRps4 infection in Arabidopsis thaliana. The work incorporates a variety of approaches from molecular biology, confocal imaging, and biochemistry, which together strengthen the conclusions.

   Reviewed by eLife

3. Khdc3 Regulates Metabolism Across Generations in a DNA-Independent Manner

   This article has 10 authors:

   1. Liana Senaldi
   2. Nora Hassan
   3. Sean Cullen
   4. Uthra Balaji
   5. Natalie Trigg
   6. Jinghua Gu
   7. Hailey Finkelstein
   8. Kathryn Phillips
   9. Colin Conine
   10. Matthew Smith-Raska

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important mouse study shows that wild-type female progeny of Khdc3 mutants have abnormal gene expression relating to hepatic metabolism, which persists over multiple generations and passes through both female and male lineages. Information about litter size and a full phenotypic description of the phenotype of each progeny should be included to evaluate the impact of KHDC3 mutation on the progeny; in its current state, the evidence for the authors' claims is incomplete. A role for small RNAs on this phenomenon is proposed but has not been functionally validated. The work will be of interest to researchers in the field of DNA-independent mechanism of inheritance. Mentioning the experimental organism in title and abstract would ensure that it targets the appropriate audience.

   Reviewed by eLife

4. A shade-responsive microProtein in the Arabidopsis ATHB2 gene regulates elongation growth and root development

   This article has 12 authors:

   1. Ashleigh Edwards
   2. Maurizio Junior Chiurazzi
   3. Anko Blaakmeer
   4. Ylenia Vittozzi
   5. Ashish Sharma
   6. Sanne Matton
   7. Valdeko Kruusvee
   8. Daniel Straub
   9. Giovanna Sessa
   10. Monica Carabelli
   11. Giorgio Morelli
   12. Stephan Wenkel

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     Through a genome-wide screen for functional alternative transcription start sites (TSS) in Arabidopsis, the authors provide evidence for widespread transcription of potential microproteins from previously annotated protein-coding genes. Functional analysis of AtHB2-miP, derived from the C-terminal region of transcription factor AtHB2 and predicted to form non-productive dimers with ATHB2, suggested that this microprotein could affect AtHB2 functions in shade responses, root growth, and iron homeostasis. The work is valuable as a case study of how new microproteins could act to modulate gene regulation in response to environmental change, but the focus on a single gene, the lack of precision in AtHB2-miP measurement and missing controls, and the relatively minor phenotypic effects mean that data supporting microprotein production as a vital regulatory strategy are incomplete.

   Reviewed by eLife

5. The domesticated transposon protein L1TD1 associates with its ancestor L1 ORF1p to promote LINE-1 retrotransposition

   This article has 14 authors:

   1. Gülnihal Kavaklioglu
   2. Alexandra Podhornik
   3. Terezia Vcelkova
   4. Jelena Marjanovic
   5. Mirjam A Beck
   6. Trinh Phan-Canh
   7. Theresia Mair
   8. Claudia Miccolo
   9. Aleksej Drino
   10. Mirko Doni
   11. Gerda Egger
   12. Susanna Chiocca
   13. Miha Modic
   14. Christian Seiser

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important paper reports functional interactions between L1TD1, an RNA binding protein (RBP), and its ancestral LINE-1 retrotransposon which is not modulated at the translational level. The evidence for the association between L1TD1 and LINE-1 ORF1p is solid. The work implies that the transposon-derived RNA binding protein in the human genome can interact with the ancestral transposable element from which this protein was initially derived. This work spurs interesting questions for cancer types, where LINE1 and L1TD1 are aberrantly expressed.

   Reviewed by eLife

6. A modular platform to display multiple hemagglutinin subtypes on a single immunogen

   This article has 7 authors:

   1. Dana Thornlow Lamson
   2. Faez Amokrane Nait Mohamed
   3. Mya Vu
   4. Daniel P Maurer
   5. Larance Ronsard
   6. Daniel Lingwood
   7. Aaron G Schmidt

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable manuscript describes the immunogenicity of a bead-on-a-string immunogen that allows the inclusion of multiple HA subtypes. The evidence to support the claims is convincing, and more importantly, this approach could be adapted to other vaccine platforms.

   Reviewed by eLife

7. Pectin methylesterase activity is required for RALF1 peptide signalling output

   This article has 8 authors:

   1. Ann-Kathrin Rößling
   2. Kai Dünser
   3. Chenlu Liu
   4. Susan Lauw
   5. Marta Rodriguez-Franco
   6. Lothar Kalmbach
   7. Elke Barbez
   8. Jürgen Kleine-Vehn

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This fundamental study provides convincing evidence for pectin modification as a requirement for RALF peptide signalling altering the apoplastic pH, adding further support for a key role of RALF peptides in linking the assembly and dynamics of the extracellular matrix with cellular activity and function. Data that have been added in comparison to a previous version have enhanced the study. The study should be of interest to anyone studying signaling and specifically to plant cell biologists.

   Reviewed by eLife

8. Modeling collective behavior in groups of mice housed under semi-naturalistic conditions

   This article has 6 authors:

   1. Xiaowen Chen
   2. Maciej Winiarksi
   3. Alicja Puścian
   4. Ewelina Knapska
   5. Thierry Mora
   6. Aleksandra M Walczak

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable work investigates the social interactions of mice living together in a system of multiple connected cages. It provides solid evidence for a statistical approach capturing changes in social interactions after manipulating prefrontal cortical plasticity. This research will be of broad interest to researchers studying animal social behavior.

   Reviewed by eLife

9. Unraveling the role of urea hydrolysis in salt stress response during seed germination and seedling growth in Arabidopsis thaliana

   This article has 8 authors:

   1. Yuanyuan Bu
   2. Xingye Dong
   3. Rongrong Zhang
   4. Xianglian Shen
   5. Yan Liu
   6. Shu Wang
   7. Tetsuo Takano
   8. Shenkui Liu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study advances our understanding of the molecular mechanism underlying salt stress-induced inhibition of seed germination and seedling growth. The evidence supporting the conclusions is convincing, with rigorous genetic, physiological, and metabolic analyses. This paper will be of interest to plant stress biologists and crop breeders.

   Reviewed by eLife

10. Caenorhabditis elegans SEL-5/AAK1 regulates cell migration and cell outgrowth independently of its kinase activity

    This article has 5 authors:

    1. Filip Knop
    2. Apolena Zounarová
    3. Vojtěch Šabata
    4. Teije Corneel Middelkoop
    5. Marie Macůrková

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      This useful study defines developmental roles for a protein kinase involved in endocytosis and reports a surprising finding that the kinase catalytic activity is unnecessary. However, several claims of the authors are only partially supported by the data. Although in its current form, this work is incomplete, it will be of broad interest to cell biologists and biochemists because this kinase was previously suggested to be a target of drug design efforts.

    Reviewed by eLife