eLife

Latest preprint reviews

1. Interneuron FGF13 regulates seizure susceptibility via a sodium channel-independent mechanism

   This article has 12 authors:

   1. Susan Lin
   2. Aravind R Gade
   3. Hong-Gang Wang
   4. James E Niemeyer
   5. Allison Galante
   6. Isabella DiStefano
   7. Patrick Towers
   8. Jorge Nunez
   9. Maiko Matsui
   10. Theodore H Schwartz
   11. Anjali Rajadhyaksha
   12. Geoffrey S Pitt

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study advances our understanding of how FGF13 variants confer seizure susceptibility. By acting in a set of inhibitory interneurons, FGF13 regulates synaptic transmission and excitability. The data presented here are convincing and combine cell type-specific knockouts and electrophysiology, complemented by histology/RNA studies. Collectively, this research will be of interest to a wide audience, particularly those involved in the study of epilepsy, inhibitory neurons, and ion channels.

   Reviewed by eLife

2. A deep learning approach for automated scoring of the Rey–Osterrieth complex figure

   This article has 21 authors:

   1. Nicolas Langer
   2. Maurice Weber
   3. Bruno Hebling Vieira
   4. Dawid Strzelczyk
   5. Lukas Wolf
   6. Andreas Pedroni
   7. Jonathan Heitz
   8. Stephan Müller
   9. Christoph Schultheiss
   10. Marius Troendle
   11. Juan Carlos Arango Lasprilla
   12. Diego Rivera
   13. Federica Scarpina
   14. Qianhua Zhao
   15. Rico Leuthold
   16. Flavia Wehrle
   17. Oskar Jenni
   18. Peter Brugger
   19. Tino Zaehle
   20. Romy Lorenz
   21. Ce Zhang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The methods and findings of the current work are important and well-grounded. The strength of the evidence presented is convincing and backed up by rigorous methodology. The work, when elaborated on how to access the app, will have far-reaching implications for current clinical practice.

   Reviewed by eLife

3. Streamlining segmentation of cryo-electron tomography datasets with Ais

   This article has 4 authors:

   1. Mart GF Last
   2. Leoni Abendstein
   3. Lenard M Voortman
   4. Thomas H Sharp

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This work describes a new software platform for machine-learning-based segmentation of and particle-picking in cryo-electron tomograms. The program and its corresponding online database of trained models will allow experimentalists to conveniently test different models and share their results with others. The paper provides convincing evidence that the software will be valuable to the community.

   Reviewed by eLife

4. Molecular, cellular, and developmental organization of the mouse vomeronasal organ at single cell resolution

   This article has 8 authors:

   1. Max Henry Hills
   2. Limei Ma
   3. Ai Fang
   4. Thelma Chiremba
   5. Seth Malloy
   6. Allison R Scott
   7. Anoja G Perera
   8. C Ron Yu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The manuscript by Hills, et al. presents data that support multiple conclusions regarding the gene expression patterns of cells, especially chemosensory neurons. The evidence is largely solid, with transcriptomic analysis combined and validated by spatially resolved expression in tissue sections, but is incomplete in other ways with some claims not fully supported. This large-scale single-cell transcriptomics dataset is an important resource, alongside a thorough exploration of the molecular features of the different cell types within the mouse vomeronasal organ, including expression of chemosensory receptors.

   Reviewed by eLife

5. Benchmarking tRNA-Seq quantification approaches by realistic tRNA-Seq data simulation identifies two novel approaches with higher accuracy

   This article has 4 authors:

   1. Tom Smith
   2. Mie Monti
   3. Anne E Willis
   4. Lajos Kalmár

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides an important resource by thoroughly benchmarking multiple sequencing-based tRNA quantification methods. The suggested best practice is supported by convincing evidence from in silico experiments in multiple scenarios.

   Reviewed by eLife

6. Engineering PEG10-assembled endogenous virus-like particles with genetically encoded neoantigen peptides for cancer vaccination

   This article has 11 authors:

   1. Ruijing Tang
   2. Luobin Guo
   3. Tingyu Wei
   4. Tingting Chen
   5. Huan Yang
   6. Honghao Ye
   7. Fangzhou Lin
   8. Yongyi Zeng
   9. Haijun Yu
   10. Zhixiong Cai
   11. Xiaolong Liu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study presents a valuable strategy to co-deliver peptides and adjuvants to antigen-presenting cells by engineering the Virus-like particle (VLP). The evidence supporting the claims of the authors is convincing, but the antitumour efficacy is unimpressive and would benefit from more antitumor experiments. The work will be of broad interest to bioengineers and medical biologists focusing on cancer vaccines.

   Reviewed by eLife

7. The Kv2.2 channel mediates the inhibition of prostaglandin E2 on glucose-stimulated insulin secretion in pancreatic β-cells

   This article has 12 authors:

   1. Chengfang Pan
   2. Ying Liu
   3. Liangya Wang
   4. Wen-Yong Fan
   5. Yunzhi Ni
   6. Xuefeng Zhang
   7. Di Wu
   8. Chenyang Li
   9. Jin Li
   10. Zhaoyang Li
   11. Rui Liu
   12. Changlong Hu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The study presents valuable findings on the molecular mechanisms of glucose-stimulated insulin secretion from pancreatic islets, focusing on the main regulatory elements of the signaling pathway in physiological conditions. While the evidence supporting the conclusions is solid, the study can be strengthened by the use of a beta cell line or knockout mice. The work will be of interest to cell biologists and biochemists working on diabetes.

   Reviewed by eLife

8. Disordered proteins interact with the chemical environment to tune their protective function during drying

   This article has 10 authors:

   1. Shraddha KC
   2. Kenny H Nguyen
   3. Vincent Nicholson
   4. Annie Walgren
   5. Tony Trent
   6. Edith Gollub
   7. Paulette Sofia Romero-Perez
   8. Alex S Holehouse
   9. Shahar Sukenik
   10. Thomas C Boothby

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study investigates the sensitivity to endogenous cosolvents of three families of intrinsically disordered proteins involved with desiccation. The findings, drawn from well-designed experiments and calculations, suggest a functional synergy between sensitivity to small molecule solutes and convergent desiccation protection strategy. The evidence is found to be convincing, and the authors provide appropriate caveats since the study's conclusions are based on a small number of proteins. This work will be of interest to biochemists and biophysicists interested in the conformation-function relationship of intrinsically disordered proteins.

   Reviewed by eLife

9. Dual-specific autophosphorylation of kinase IKK2 enables phosphorylation of substrate IκBα through a phosphoenzyme intermediate

   This article has 9 authors:

   1. Prateeka Borar
   2. Tapan Biswas
   3. Ankur Chaudhuri
   4. Pallavi T Rao
   5. Swasti Raychaudhuri
   6. Tom Huxford
   7. Saikat Chakrabarti
   8. Gourisankar Ghosh
   9. Smarajit Polley

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents fundamental findings that could redefine the specificity and mechanism of action of the well-studied Ser/Thr kinase IKK2 (a subunit of inhibitor of nuclear factor kappa-B kinase (IkB) that propagates cellular response to inflammation). Solid evidence supports the claim that IKK2 exhibits dual specificity that allows tyrosine autophosphorylation and the authors further show that auto-phosphorylated IKK2 is involved in an unanticipated relay mechanism that transfers phosphate from an IKK2 tyrosine onto the IkBa substrate. The findings are a starting point for follow-up studies to confirm the unexpected mechanism and further pursue functional significance.

   Reviewed by eLife

10. Human airway macrophages are metabolically reprogrammed by IFN-γ resulting in glycolysis-dependent functional plasticity

    This article has 16 authors:

    1. Donal J Cox
    2. Sarah A Connolly
    3. Cilian Ó Maoldomhnaigh
    4. Aenea AI Brugman
    5. Olivia Sandby Thomas
    6. Emily Duffin
    7. Karl M Gogan
    8. Oisin Ó Gallchobhair
    9. Dearbhla M Murphy
    10. Sinead A O'Rourke
    11. Finbarr O'Connell
    12. Parthiban Nadarajan
    13. James J Phelan
    14. Laura E Gleeson
    15. Sharee A Basdeo
    16. Joseph Keane

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      In this valuable study, the authors investigate how inflammatory priming and exposure to irradiated Mycobacterium tuberculosis or the bacterial endotoxin LPS impact the metabolism of primary human airway macrophages and monocyte-derived macrophages. The work shows that metabolic plasticity is greater in monocyte-derived macrophages than alveolar macrophages, with solid experimental methods and overall evidence. The findings are relevant to the field of immunometabolism.

    Reviewed by eLife