eLife

Latest preprint reviews

1. Endogenous oligomer formation underlies DVL2 condensates and promotes Wnt/β-catenin signaling

   This article has 7 authors:

   1. Senem Ntourmas
   2. Martin Sachs
   3. Petra Paclíková
   4. Martina Brückner
   5. Vítězslav Bryja
   6. Jürgen Behrens
   7. Dominic B Bernkopf

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study contributes to the understanding of phase separation in Dishevelled (DVL) proteins, by investigating the endogenous complexes of DVL2 using ultracentrifugation and contrasting them with DVL1 and DVL3 behaviour and the functional validation of the DVL2 intrinsically disordered regions mediating the protein condensate. The study includes a solid characterisation of several overexpression constructs, including in KO cells. However, investigations of the roles of the described DVL2 regions at the endogenous level remain to be carried out.

   Reviewed by eLife

2. Knockout of cyclin-dependent kinases 8 and 19 leads to depletion of cyclin C and suppresses spermatogenesis and male fertility in mice

   This article has 23 authors:

   1. Alexandra V Bruter
   2. Ekaterina A Varlamova
   3. Nina I Stavskaya
   4. Zoia G Antysheva
   5. Vasily N Manskikh
   6. Anna V Tvorogova
   7. Diana S Korshunova
   8. Alvina I Khamidullina
   9. Marina V Utkina
   10. Viktor P Bogdanov
   11. Iuliia P Baikova
   12. Alyona I Nikiforova
   13. Eugene A Albert
   14. Denis O Maksimov
   15. Jing Li
   16. Mengqian Chen
   17. Gary P Schools
   18. Alexey V Feoktistov
   19. Alexander A Shtil
   20. Igor B Roninson
   21. Vladislav A Mogila
   22. Yulia Y Silaeva
   23. Victor V Tatarskiy

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study reports the critical role of two cyclin-dependent kinases, CDK8 and CDK19, in spermatogenesis. The data presented are generally supportive of the main conclusion and are considered solid. This work may be of interest to reproductive biologists and physicians working on male fertility.

   Reviewed by eLife

3. The interplay between biomolecular assembly and phase separation

   This article has 4 authors:

   1. Giacomo Bartolucci
   2. Ivar S Haugerud
   3. Thomas CT Michaels
   4. Christoph A Weber

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The authors present an important theoretical framework that describes the interplay between liquid-liquid phase separation and protein aggregation within a mean-field model. This work will be of high interest to the biophysics and molecular biology communities, as it will help understand and analyse assembly within biomolecular condensates in cells or in-vitro. Major strengths of this convincing work are the consideration of aggregates with various dimensionality and the possibility for protein gelation.

   Reviewed by eLife

4. CTFFIND5 provides improved insight into quality, tilt, and thickness of TEM samples

   This article has 4 authors:

   1. Johannes Elferich
   2. Lingli Kong
   3. Ximena Zottig
   4. Nikolaus Grigorieff

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable work presents the latest version of CTFFIND, which is the most popular software for determination of the contrast transfer function (CTF) in cryo-electron microscopy. CTFFIND5 estimates and considers acquisition geometry and sample thickness, which leads to improved CTF determination. The paper describes compelling evidence that CTFFIND5 finds better CTF parameters than previous methods, in particular for tilted samples (e.g. for cryo-electron tomography) or where thickness is an issue (e.g. cellular samples, or electron microscopy at low voltages).

   Reviewed by eLife

5. CXCR3-expressing myeloid cells recruited to the hypothalamus protect against diet-induced body mass gain and metabolic dysfunction

   This article has 10 authors:

   1. Natalia Mendes
   2. Ariane Zanesco
   3. Cristhiane Aguiar
   4. Gabriela F Rodrigues-Luiz
   5. Dayana Silva
   6. Jonathan Campos
   7. Niels Olsen Saraiva Camara
   8. Pedro Moraes-Vieira
   9. Eliana Araujo
   10. Licio A Velloso

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This work is of fundamental significance and has an exceptional level of evidence for a new population that protects against obesity-induced hypothalamic inflammation. This topic will attract attention from a broad base of readers, from hypothalamic neuroscientists to immunologists with an interest in metabolism.

   Reviewed by eLife

6. Using normative models pre-trained on cross-sectional data to evaluate intra-individual longitudinal changes in neuroimaging data

   This article has 8 authors:

   1. Barbora Rehak Buckova
   2. Charlotte Fraza
   3. Rastislav Rehák
   4. Marián Kolenič
   5. Christian F Beckmann
   6. Filip Španiel
   7. Andre F Marquand
   8. Jaroslav Hlinka

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This paper addresses an important topic (normative trajectory modelling), seeking to provide a method aiming to accurately reflect the individual deviation of longitudinal/temporal change compared to the normal temporal change characterized based on a pre-trained population normative model. The evidence provided for the new methods is solid.

   Reviewed by eLife

7. Psychological stress disturbs bone metabolism via miR-335-3p/Fos signaling in osteoclast

   This article has 8 authors:

   1. Jiayao Zhang
   2. Juan Li
   3. Jiehong Huang
   4. Xuerui Xiang
   5. Ruoyu Li
   6. Yun Zhai
   7. Shuxian Lin
   8. Weicai Liu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The manuscript presents valuable findings of bone remodeling under chronic unpredictable mild stress (CUMS). This is an interesting work on mental stress on bone health and osteoporosis, and the authors offer solid evidence of decreased bone mass mediated by miR-335-3p/Fos signaling in osteoclasts that are involved in the induction of bone loss caused by CUMS. This revised version provided new data that improved the quality of the manuscript and addressed the reviewers' concerns.

   Reviewed by eLife

8. Lineage-specific intersection of endothelin and GDNF signaling in enteric nervous system development

   This article has 5 authors:

   1. Denise M Poltavski
   2. Alexander T Cunha
   3. Jaime Tan
   4. Henry M Sucov
   5. Takako Makita

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides valuable insights into our understanding of the development of the enteric nervous system. The authors use genetically engineered mice to study the behavior of stem cells in organizing the enteric nervous system and the secreted signals that regulate these cells. The study rests on a degree of incomplete evidence since the characterization of some of the mouse resources is not complete in the current version.

   Reviewed by eLife

9. The full-length BEND2 protein is dispensable for spermatogenesis but required for setting the ovarian reserve in mice

   This article has 14 authors:

   1. Yan Huang
   2. Nina Bucevic
   3. Carmen Coves
   4. Natalia Felipe-Medina
   5. Marina Marcet-Ortega
   6. Nikoleta Nikou
   7. Cristina Madrid-Sandín
   8. Maria López-Panadés
   9. Carolina Buza
   10. Neus Ferrer Miralles
   11. Antoni Iborra
   12. Anna Pujol
   13. Alberto M Pendás
   14. Ignasi Roig

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides valuable information on a novel gene that regulates meiotic progression in both male and female meiosis. The evidence supporting the conclusions of the authors is solid. This study will be of interest to developmental and reproductive biologists.

   Reviewed by eLife

10. Evaluation of clonal hematopoiesis and mosaic loss of Y chromosome in cardiovascular risk: An analysis in prospective studies

    This article has 15 authors:

    1. Sami Fawaz
    2. Severine Marti
    3. Melody Dufossee
    4. Yann Pucheu
    5. Astrid Gaufroy
    6. Jean Broitman
    7. Audrey Bidet
    8. Aicha Soumare
    9. Gaëlle Munsch
    10. Christophe Tzourio
    11. Stephanie Debette
    12. David-Alexandre Trégouët
    13. Chloe James
    14. Olivier Mansier
    15. Thierry Couffinhal

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      In this small study involving patients with a history of myocardial infarction, Fawaz et al. found no significant contribution of clonal hematopoiesis and mosaic loss of the Y chromosome to the incidence of myocardial infarction and atherosclerosis. Although the evidence provided by the study is incomplete due to its small sample size, the findings are valuable for guiding future larger studies that will further investigate this significant and controversial subject.

    Reviewed by eLife