eLife

Latest preprint reviews

1. A delta-tubulin/epsilon-tubulin/Ted protein complex is required for centriole architecture

   This article has 8 authors:

   1. Rachel Pudlowski
   2. Lingyi Xu
   3. Ljiljana Milenkovic
   4. Chandan Kumar
   5. Katherine Hemsworth
   6. Zayd Aqrabawi
   7. Tim Stearns
   8. Jennifer T Wang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The study by Pudlowski et al. shows that a previously-identified protein complex, composed of delta- and epsilon-tubulin together with TEDC1 and TEDC2, functions in generating centriolar triplet microtubules, and that this is crucial for the proper formation of centriolar subdomains and the stability of centrioles throughout the cell cycle. This is an important study that advances our understanding of centriole biogenesis and structure and is supported by convincing evidence based on knockout cell lines, immunoprecipitation, and ultrastructure expansion microscopy. The work is of interest to cell biologists, in particular researchers with interest in centrosome biology.

   Reviewed by eLife

2. The emergence and evolution of gene expression in genome regions replete with regulatory motifs

   This article has 3 authors:

   1. Timothy Fuqua
   2. Yiqiao Sun
   3. Andreas Wagner

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study explores the relationship between the sequence of prokaryotic promoter elements and their activity using mutagenesis to generate thousands of mutant sequences. The evidence supporting these findings is convincing. This work will appeal to those interested in bacterial genetics, genome evolution, and gene regulation.

   Reviewed by eLife

3. VGLL2 and TEAD1 fusion proteins identified in human sarcoma drive YAP/TAZ-independent tumorigenesis by engaging EP300

   This article has 13 authors:

   1. Susu Guo
   2. Xiaodi Hu
   3. Jennifer L Cotton
   4. Lifang Ma
   5. Qi Li
   6. Jiangtao Cui
   7. Yongjie Wang
   8. Ritesh P Thakare
   9. Zhipeng Tao
   10. Y Tony Ip
   11. Xu Wu
   12. Jiayi Wang
   13. Junhao Mao

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This is a valuable study describing how rhabdomyosarcoma fusion-oncogenes, VGLL2-NCOA2 and TEAD1-NCOA2, function at the genomic, transcriptional, and proteomic levels in multiple systems. The experimental data is convincing, supporting a model in which these fusion-oncogenes leverage TEAD transcriptional signatures independent of YAP/TAZ. This work offers new mechanistic insights into oncogenic gene fusion events and reveals potential therapeutic strategies for the treatment of rhabdomyosarcomas.

   Reviewed by eLife

4. Rudhira-mediated microtubule stability controls TGFβ signaling during mouse vascular development

   This article has 4 authors:

   1. Divyesh Joshi
   2. Preeti Jindal
   3. Ronak K Shetty
   4. Maneesha S Inamdar

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important work provides another layer of regulatory mechanism for TGF-beta signaling activity. The evidence convincingly supports the involvement of microtubules as a reservoir of Smad2/3, and association of Rudhira with microtubules is critical for this process. The work will be of board interest to developmental biologists in general and molecular biologists in the field of growth factor signaling.

   Reviewed by eLife

5. Conditional Survival and Nomogram for Elderly Non-Metastatic Colon Cancer Patients Following Colectomy

   This article has 6 authors:

   1. Yadong Gao
   2. Huimin Wang
   3. Yi Zhang
   4. Jing Zhao
   5. Sujuan Feng
   6. Jianwei Qiu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This useful work provides a risk-prediction tool, in the form of a nomogram, for practitioners and elderly patients with non-metastatic colon cancer using data from the SEER registry. The unique contribution of this work is the focus on conditional survival. However, the underlying statistical approach is suboptimal and therefore incomplete, which substantially lessens the potential impact of this work. The analysis could use a more rigorous consideration of competing risks.

   Reviewed by eLife

6. NPRL2 gene therapy induces effective antitumor immunity in KRAS/STK11 mutant anti-PD1 resistant metastatic non-small cell lung cancer (NSCLC) in a humanized mouse model

   This article has 9 authors:

   1. Ismail M Meraz
   2. Mourad Majidi
   3. Renduo Song
   4. Feng Meng
   5. Lihui Gao
   6. Qi Wang
   7. Jing Wang
   8. Elizabeth J Shpall
   9. Jack A Roth

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study provides a novel and promising NPRL2 gene therapy for enhanced immunotherapy response in a KRAS/STK11 mutant anti-PD1 resistant metastatic NSCLC humanized mouse model. Overall, the authors presented a large amount of convincing in vivo data to demonstrate that NPRL2 gene therapy induces antitumor activity through DC-mediated antigen presentation and cytotoxic immune cell activation. This work will be of interest and useful to medical biologists and oncologists in the research field of KRAS-mutant NSCLC.

   Reviewed by eLife

7. Timing of treatment shapes the path to androgen receptor signaling inhibitor resistance in prostate cancer

   This article has 11 authors:

   1. Eugine Lee
   2. Zeda Zhang
   3. Chi-Chao Chen
   4. Danielle Choi
   5. Aura C. Agudelo Rivera
   6. Eliot Linton
   7. Yu-jui Ho
   8. Jillian Love
   9. Justin LaClair
   10. John Wongvipat
   11. Charles L. Sawyers

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study provides new insight into the dynamics that underlie the development of therapy resistance in prostate cancer by revealing that divergent tumor evolutionary paths occur in response to different treatment timing and that these converge on common resistance mechanisms. The use of barcoded lineage tracing and characterization of isolated tumor clonal populations provides compelling evidence supporting the importance of clonal dynamics in a tumor ecosystem for treatment resistance. Several open questions remain, however, raising the possibility of alternative interpretations of the data set in its current form. Overall, the findings deepen our understanding of prostate cancer evolution and hold promising implications for how drug resistance can be addressed or prevented.

   Reviewed by eLife

8. Partitioning changes in ecosystem productivity by effects of species interactions in biodiversity experiments

   This article has 9 authors:

   1. Jing Tao
   2. Charles A Nock
   3. Eric B Searle
   4. Shongming Huang
   5. Rongzhou Man
   6. Hua Yang
   7. Grégoire T Freschet
   8. Cyrille Violle
   9. Ji Zheng

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The authors propose that positive biodiversity-ecosystem functioning relationships found in experiments have been exaggerated because commonly used statistical analyses are flawed. To remedy this, a new type of analysis based on a concept of "partial density monoculture yield" is proposed. However, the presented concept and analysis methods are not reproducibly described (how can partial density monoculture yield experimentally be assessed?), do not appear to be complete, and are inadequate for hypothesis testing. The reviewers found that the authors misinterpret current research in the field and made limited efforts to understand or address the reviewer comments about this study.

   Reviewed by eLife

9. Sex-dependent, lateralized engagement of anterior insular cortex inputs to the dorsolateral striatum in binge alcohol drinking

   This article has 2 authors:

   1. David L Haggerty
   2. Brady K Atwood

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This valuable manuscript describes evidence of sex differences in specific corticostriatal projections during alcohol consumption, and this is noteworthy given the increasing rates/levels of drinking in females and their liability for Alcohol Use disorder. The authors provide solid evidence of the lateralisation of the activity of the circuit, but other evidence is incomplete, particularly with regard to how the drinking measure relates to intoxication. There are some inconsistencies that make it difficult to reconcile the photometry and behavioral data. The findings would benefit from causal assessment in the future. The findings will be of interest to researchers investigating functional circuitry underlying alcohol-driven behaviors.

   Reviewed by eLife

10. Novel mechanism for tubular injury in nephropathic cystinosis

    This article has 7 authors:

    1. Swastika Sur
    2. Maggie Kerwin
    3. Silvia Pineda
    4. Poonam Sansanwal
    5. Tara K Sigdel
    6. Marina Sirota
    7. Minnie M Sarwal

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This important study addresses the idea that defective lysosomal clearance might be causal to renal dysfunction in cystinosis. With mostly solid data, the authors observe that restoring expression of vATPase subunits and treatment with Astaxanthin ameliorate mitochondrial function in a model of renal epithelial cells, opening opportunities for translational application to humans.

    Reviewed by eLife