eLife

Latest preprint reviews

1. Development of a new genotype–phenotype linked antibody screening system

   This article has 9 authors:

   1. Takashi Watanabe
   2. Hikaru Hata
   3. Yoshiki Mochizuki
   4. Fumie Yokoyama
   5. Tomoko Hasegawa
   6. Naveen Kumar
   7. Tomohiro Kurosaki
   8. Osamu Ohara
   9. Hidehiro Fukuyama

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The useful studies described here are broadly applicable to all antibody discovery subfields, even though they are not a significant improvement over published methods. The findings are incomplete with respect to the methodology, since details that are crucial in order to repeat the experiments are lacking (such as a timestamp). They also do not take into account multiple recent papers that have tested similar strategies. These studies will be of interest to a specialized audience working on generating antibodies to infectious agents.

   Reviewed by eLife

2. Evolution of hind limb morphology of Titanosauriformes (Dinosauria, Sauropoda) analyzed via 3D geometric morphometrics reveals wide-gauge posture as an exaptation for gigantism

   This article has 4 authors:

   1. Adrián Páramo
   2. Pedro Mocho
   3. Fernando Escaso
   4. Francisco Ortega

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The authors present convincing findings on trends in hind limb morphology through the evolution of titanosaurian sauropod dinosaurs, the land animals that reached the most remarkable gigantic sizes. The important results include the use of 3D geometric morphometrics to examine the femur, tibia, and fibula to provide new information on the evolution of this clade and on evolutionary trends between morphology and allometry.

   Reviewed by eLife

3. The PRC2.1 subcomplex opposes G1 progression through regulation of CCND1 and CCND2

   This article has 15 authors:

   1. Adam D Longhurst
   2. Kyle Wang
   3. Harsha Garadi Suresh
   4. Mythili Ketavarapu
   5. Henry N Ward
   6. Ian R Jones
   7. Vivek Narayan
   8. Frances V Hundley
   9. Arshia Zernab Hassan
   10. Charles Boone
   11. Chad L Myers
   12. Yin Shen
   13. Vijay Ramani
   14. Brenda J Andrews
   15. David P Toczyski

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study reports a chemogenetic screen for resistance and sensitivity to three cell cycle inhibitors used in the clinic: camptothecin, colchicine, and palbociclib. The screen provides a wealth of information that will be of interest to cell cycle and cancer biologists. Convincing evidence is provided that resistance to palbociclib can result from loss of PRC2.1 activity, which raises cyclin D levels. The effect of PRC2.1 on cyclin D is not universal across tested cell lines with the causal differences not yet understood.

   Reviewed by eLife

4. A genome-wide CRISPR/Cas9 screen identifies calreticulin as a selective repressor of ATF6α

   This article has 6 authors:

   1. Joanne Tung
   2. Lei Huang
   3. Ginto George
   4. Heather P Harding
   5. David Ron
   6. Adriana Ordonez

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     In this important study, the authors explore ER stress signaling mediated by ATF6 using a genome-wide gene depletion screen. They find that the ER chaperone Calreticulin binds and directly represses ATF6, a new and intriguing function for Calreticulin. The evidence presented is convincing, based on CHO genetics and biochemical analysis.

   Reviewed by eLife

5. Probing PAC1 receptor activation across species with an engineered sensor

   This article has 14 authors:

   1. Reto B Cola
   2. Salome N Niethammer
   3. Preethi Rajamannar
   4. Andrea Gresch
   5. Musadiq A Bhat
   6. Kevin Assoumou
   7. Elyse T Williams
   8. Patrick Hauck
   9. Nina Hartrampf
   10. Dietmar Benke
   11. Miriam Stoeber
   12. Gil Levkowitz
   13. Sarah Melzer
   14. Tommaso Patriarchi

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This fundamental paper reports a new biosensor to study G protein-coupled receptor activation by the pituitary adenylyl cyclase-activating polypeptide (PACAP) in cell culture, ex vivo (mouse brain slices), and in vivo (zebrafish, mouse). Convincing data are presented that show the new sensor works with high affinity in vitro, while requiring very high (non-physiological) concentrations of exogenous PACAP when applied to intact tissues. The sensor has not yet been used to detect endogenously released PACAP, raising questions about whether the sensor can be used for its intended purpose. While further work must be pursued to achieve broad in vivo applications under physiological conditions, the new tool will be of interest to cell biologists, especially those studying the large and significant GPCR family.

   Reviewed by eLife

6. Visualization of endogenous G proteins on endosomes and other organelles

   This article has 5 authors:

   1. Wonjo Jang
   2. Kanishka Senarath
   3. Gavin Feinberg
   4. Sumin Lu
   5. Nevin A Lambert

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study investigates the intracellular localization patterns of G proteins involved in GPCR signaling, presenting compelling evidence for their preference for plasma and lysosomal membranes over endosomal, endoplasmic reticulum, and Golgi membranes. This discovery has significant implications for understanding GPCR action and signaling from intracellular locations. This research will interest cell biologists studying protein trafficking and pharmacologists exploring localized signaling phenomena.

   Reviewed by eLife

7. Characterisation and comparison of semen microbiota and bacterial load in men with infertility, recurrent miscarriage, or proven fertility

   This article has 19 authors:

   1. Shahriar Mowla
   2. Linda Farahani
   3. Tharu Tharakan
   4. Rhianna Davies
   5. Goncalo DS Correia
   6. Yun S Lee
   7. Samit Kundu
   8. Shirin Khanjani
   9. Emad Sindi
   10. Raj Rai
   11. Lesley Regan
   12. Dalia Khalifa
   13. Ralf Henkel
   14. Suks Minhas
   15. Waljit S Dhillo
   16. Jara Ben Nagi
   17. Phillip Bennett
   18. David A MacIntyre
   19. Channa N Jayasena

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study reports a potential connection between the seminal microbiome and sperm quality/male fertility. The data are generally convincing. This study will be of interest to clinicians and biomedical researchers who work on microbiome and male fertility.

   Reviewed by eLife

8. Novel 3D Approach to Model Non-Alcoholic Fatty Liver Disease using human Pluripotent Stem Cells

   This article has 18 authors:

   1. Carola Maria Morell
   2. Samantha Grace Tilson
   3. Rute Alexandra Tomaz
   4. Arash Shahsavari
   5. Andi Munteanu
   6. Giovanni Canu
   7. Brandon Tyler Wesley
   8. Marion Perrin
   9. Imbisaat Geti
   10. Subhankar Mukhopadhyay
   11. Francesca Mazzacuva
   12. Paul Gissen
   13. Jose Garcia-Bernardo
   14. Martin Bachman
   15. Casey Allison Rimland
   16. Fotios Sampaziotis
   17. Irina Mohorianu
   18. Ludovic Vallier

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The authors present an important study of a multi-cellular platform involving co-culturing of various hiPSC-derived hepatocyte like cells, cholangiocytes, stellate cells and macrophages to mimic the liver microenvironment. The aggregates are then treated with fatty acids and examined through transcriptomic and functional assays. The techniques and methodologically are sound, and the evidence supporting the conclusion is convincing, although more clinically relevant data demonstrating the effect of some potential pharmacological agents on the platform would serve to strengthen the study.

   Reviewed by eLife

9. Transcription factor condensates, 3D clustering, and gene expression enhancement of the MET regulon

   This article has 7 authors:

   1. James Lee
   2. Leman Simpson
   3. Yi Li
   4. Samuel Becker
   5. Fan Zou
   6. Xin Zhang
   7. Lu Bai

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study investigates the relationship between transcription factor condensate formation, transcription, and 3D gene clustering of the MET regulon in the model organism S. cerevisiae. The authors provide solid experimental evidence that transcription factor condensates enhance transcription of MET-regulated genes, but evidence for the role of Met4 IDRs and Met4-containing condensates in mediating target gene clustering in the MET regulon is not as strong. This paper will be of interest to molecular biologists working on chromatin and transcription, although its impact would be strengthened by further investigation.

   Reviewed by eLife

10. The title and authors for this article are not available from our external data provider yet:
    10.48550/arxiv.2202.13658v4