eLife

Latest preprint reviews

1. Stimulus representation in human frontal cortex supports flexible control in working memory

   This article has 3 authors:

   1. Zhujun Shao
   2. Mengya Zhang
   3. Qing Yu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This work presents important findings that the human frontal cortex is involved in a flexible, dual role in both maintaining information in short-term memory, and controlling this memory content to guide adaptive behavior and decisions. The evidence supporting the conclusions is compelling, with a well-designed task, best-practice decoding methods, and careful control analyses. The work will be of broad interest to cognitive neuroscience researchers working on working memory and cognitive control.

   Reviewed by eLife

2. Cell chirality reversal through tilted balance between polymerization of radial fibers and clockwise-swirling of transverse arcs

   This article has 12 authors:

   1. Hoi Kwan Kwong
   2. Miu Ling Lam
   3. Siying Wu
   4. Cho Fan Chung
   5. Jianpeng Wu
   6. Lok Ting Chu
   7. King Hoo Lim
   8. Hiu Lam Chow
   9. Hogi Hartanto
   10. Wengang Liu
   11. Kwan Ting Chow
   12. Ting-Hsuan Chen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The intrinsic chirality of actin filaments (F-actin) is implicated in the chiral arrangement and movement of cellular structures, but it was unknown how opposite chiralities can arise when the chirality of F-actin is invariant. Kwong et al. present evidence that two actin filament-based cytoskeletal structures, transverse actin arcs and radial stress fibers, drive clockwise and anti-clockwise rotation, respectively. This fundamental work, which has broad implications for cell biology, is supported by compelling data.

   Reviewed by eLife

3. The title and authors for this article are not available from our external data provider yet:
   10.48550/arxiv.2312.17293v3

4. TRPML1 gating modulation by allosteric mutations and lipids

   This article has 4 authors:

   1. Ninghai Gan
   2. Yan Han
   3. Weizhong Zeng
   4. Youxing Jiang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     Transient receptor potential mucolipin 1 (TRPML1) functions as a lysosomal ion channel whose variants are associated with lysosomal storage disorder mucolipidosis type IV. This important report describes local and global structural changes driven by binding of regulatory phospholipids and by mutations that allosterically cause gain or loss of channel function. Most of the claims related to the allosteric regulation of TRPML1 are convincingly supported by two new cryo-EM structures which are evaluated within the context of previously reported TRPML1 structures, and a proposed allosteric gating mechanism is partially supported by functional electrophysiology results.

   Reviewed by eLife

5. The evolutionary modifications of a GoLoco motif in the AGS protein facilitate micromere formation in the sea urchin embryo

   This article has 4 authors:

   1. Natsuko Emura
   2. Florence DM Wavreil
   3. Annaliese Fries
   4. Mamiko Yajima

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study presents work on the molecular mechanism driving asymmetric cell division and fate decisions during embryonic development of echinoids. The evidence supporting the claims of the authors is convincing. The work will be of interest to developmental biologists and cell biologists working in the field of self-renewal.

   Reviewed by eLife

6. Deuterium metabolic imaging phenotypes mouse glioblastoma heterogeneity through glucose turnover kinetics

   This article has 9 authors:

   1. Rui Vasco Simoes
   2. Rafael Neto Henriques
   3. Jonas L Olesen
   4. Beatriz M Cardoso
   5. Francisca F Fernandes
   6. Mariana AV Monteiro
   7. Sune N Jespersen
   8. Tânia Carvalho
   9. Noam Shemesh

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides a valuable approach to image and analyze in vivo metabolic flux through glucose turnover kinetics in glioblastoma tumor microenvironments. The evidence for the method's validity is convincing, which establishes the dynamic Deuterium Metabolic Imaging technique as an effective tool enabling non-invasive exploration of various tumors.

   Reviewed by eLife

7. One N-glycan regulates natural killer cell antibody-dependent cell-mediated cytotoxicity and modulates Fc γ receptor IIIa/CD16a structure

   This article has 4 authors:

   1. Paul G Kremer
   2. Elizabeth A Lampros
   3. Allison M Blocker
   4. Adam W Barb

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study explores the mechanistic link between glycosylation at the N162 site of the Fc gamma receptor FcγRIIIa and the modulation of NK cell-mediated antibody-dependent cytotoxicity. Using innovative isotope labeling strategies and advanced NMR spectroscopy techniques, the authors provide compelling evidence of how glycan composition influences receptor stability and immune function. These findings offer fundamental insights that may contribute to the development of more effective therapeutic antibodies. The manuscript will be of significant interest to immunologists and researchers focused on therapeutic antibody design.

   Reviewed by eLife

8. Allosteric inhibition of trypanosomatid pyruvate kinases by a camelid single-domain antibody

   This article has 15 authors:

   1. Joar Esteban Pinto Torres
   2. Mathieu Claes
   3. Rik Hendrickx
   4. Meng Yuan
   5. Natalia Smiejkowska
   6. Pieter Van Wielendaele
   7. Aysima Hacisuleyman
   8. Hans De Winter
   9. Serge Muyldermans
   10. Paul AM Michels
   11. Malcolm D Walkinshaw
   12. Wim Versées
   13. Guy Caljon
   14. Stefan Magez
   15. Yann G-J Sterckx

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This work presents valuable data demonstrating that a camelid single-domain antibody can selectively inhibit a key glycolytic enzyme in trypanosomes via an allosteric mechanism. The claim that this information can be exploited for the design of novel chemotherapeutics is solid but limited by the modest effects on parasite growth, as well as the lack of evidence for cellular target engagement in vivo.

   Reviewed by eLife

9. A model-based factorization method for scRNA data unveils bifurcating transcriptional modules underlying cell fate determination

   This article has 9 authors:

   1. Jun Ren
   2. Ying Zhou
   3. Yudi Hu
   4. Jing Yang
   5. Hongkun Fang
   6. Xuejing Lyu
   7. Jintao Guo
   8. Xiaodong Shi
   9. Qiyuan Li

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     MGPfactXMBD is a novel computational method for investigating cell evolutionary trajectory for scRNA-seq samples. It is important, with several potential future applications. The authors benchmarked this method using synthetic and real-world samples and showed superior performance for some of the tasks in cell trajectory analysis compared to other methods with compelling evidence.

   Reviewed by eLife

10. Redistribution of fragmented mitochondria ensures symmetric organelle partitioning and faithful chromosome segregation in mitotic mouse zygotes

    This article has 11 authors:

    1. Haruna Gekko
    2. Ruri Nomura
    3. Daiki Kuzuhara
    4. Masato Kaneyasu
    5. Genpei Koseki
    6. Deepak Adhikari
    7. Yasuyuki Mio
    8. John Carroll
    9. Tomohiro Kono
    10. Hiroaki Funahashi
    11. Takuya Wakai

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This important study investigates the role of Drp1 in early embryo development. The authors have addressed most of the original comments and the work now presents convincing evidence on how this protein influences mitochondrial localization and partitioning during the first embryonic divisions. The research employs the Trim-Away technique to eliminate Drp1 in zygotes, revealing critical insights into mitochondrial clustering, spindle formation, and embryonic development.

    Reviewed by eLife