eLife

Latest preprint reviews

1. Distinct release properties of glutamate/GABA co-transmission serve as a frequency-dependent filtering of supramammillary inputs

   This article has 6 authors:

   1. Himawari Hirai
   2. Kohtarou Konno
   3. Miwako Yamasaki
   4. Masahiko Watanabe
   5. Takeshi Sakaba
   6. Yuki Hashimotodani

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This fundamental work provides evidence that glutamate and GABA are released from different synaptic vesicles at supramammillary axon terminals onto granule cells of the dentate gyrus. The study uses complementary electrophysiological and anatomical experimental approaches. Together, these provide convincing evidence that the co-release of glutamate and GABA from different vesicles within the same terminal could modulate granule cell firing in a frequency-dependent manner, although thorough elimination of alternative mechanisms would have strengthened the study. The work will be of interest to neuroscientists investigating co-release of neurotransmitters in various synapses in the brain and those interested in subcortical control of hippocampal function.

   Reviewed by eLife

2. Remote automated delivery of mechanical stimuli coupled to brain recordings in behaving mice

   This article has 23 authors:

   1. Justin Burdge
   2. Anissa Jhumka
   3. Ashar Khan
   4. Simon Ogundare
   5. Nicholas Baer
   6. Sasha Fulton
   7. Alexander Kaplan
   8. Brittany Bistis
   9. William Foster
   10. Joshua Thackray
   11. Andre Toussaint
   12. Miao Li
   13. Yosuke M Morizawa
   14. Jake Nazarian
   15. Leah Yadessa
   16. Arlene J George
   17. Abednego Delinois
   18. Wadzanayi Mayiseni
   19. Noah Loran
   20. Guang Yang
   21. David J Margolis
   22. Victoria E Abraira
   23. Ishmail Abdus-Saboor

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study describes the development and validation of an Automated Reproducible Mechano-stimulator (ARM), a tool for standardizing and automating tactile behavior experiments. The data supporting the use of the ARM system are compelling, and demonstrate that by removing experimenter effects on animals, it reduces variability in various parameters of stimulus application. Moreover, the authors demonstrate that any noise emitted from the ARM does not induce an increased stress state. Once commercially available, the ARM system has the potential to increase experimental reproducibility between laboratories in the somatosentation and pain fields.

   Reviewed by eLife

3. Mapping serotonergic dynamics using drug-modulated molecular connectivity in rats

   This article has 7 authors:

   1. Tudor M Ionescu
   2. Mario Amend
   3. Rakibul Hafiz
   4. Andreas Maurer
   5. Bharat Biswal
   6. Hans F Wehrl
   7. Kristina Herfert

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important paper on measuring molecular connectivity using combined serotonin PET and resting-state fMRI provides both novel methods for studying the brain as well as insights into the effects of ecstasy administration. The methods are convincing, with the high anaesthetic dose used likely limiting network activity.

   Reviewed by eLife

4. Salmonids elicit an acute behavioral response to heterothermal environments

   This article has 7 authors:

   1. Robert Naudascher
   2. Stefano Brizzolara
   3. Jonasz Slomka
   4. Robert M Boes
   5. Markus Holzner
   6. Luiz GM Silva
   7. Roman Stocker

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable paper investigates how fish avoid thermal disturbances that occur on fast timescales. The authors use a creative experimental approach that quickly creates a vertical thermal interface, which they combine with careful behavioral analyses. The evidence supporting their results is solid, but there is a potential confounding factor between temperature and vertical positioning, and characterization of the thermal interface would greatly assist in interpreting the results.

   Reviewed by eLife

5. Fish CDK2 recruits Dtx4 to degrade TBK1 through ubiquitination in the antiviral response

   This article has 16 authors:

   1. Long-Feng Lu
   2. Can Zhang
   3. Zhuo-Cong Li
   4. Bao-Jie Cui
   5. Yang-Yang Wang
   6. Ke-Jia Han
   7. Xiao Xu
   8. Chu-Jing Zhou
   9. Xiao-Yu Zhou
   10. Yue Wu
   11. Na Xu
   12. Xiao-Li Yang
   13. Dan-Dan Chen
   14. Xiyin Li
   15. Li Zhou
   16. Shun Li

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study uses zebrafish as a model to reveal a role for the cell cycle protein kinase CDK2 as a negative regulator of type I interferon signaling. The evidence supporting the authors' claims is convincing, including both in vivo and in vitro investigative approaches that corroborate a role for CDK2 in regulating TBK1 degradation. In this latest version, the authors included data addressing a concern raised by the reviewer in the previous peer review round. This work will interest cell biologists, immunologists, and virologists.

   Reviewed by eLife

6. Defining mononuclear phagocyte distribution and behaviour in the zebrafish heart

   This article has 6 authors:

   1. Bethany Moyse
   2. Joanna Moss
   3. Laura Bevan
   4. Aaron Scott
   5. Valérie Wittamer
   6. Rebecca J Richardson

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The manuscript presented by Moyse and colleagues provides valuable insight into the origin, morphology, dynamics, and behavior of several populations of mononuclear phagocytes in the zebrafish heart. The study presents solid evidence through the use of transgenic lines and live imaging, although some limitations related to lineage tracing and molecular profiles should be considered. This work exemplifies the use of zebrafish as a model to study the role of leukocytes in cardiac development and regeneration and potentially draw broader interest to biologists working in immunology fields.

   Reviewed by eLife

7. Electrophysiological dynamics of salience, default mode, and frontoparietal networks during episodic memory formation and recall revealed through multi-experiment iEEG replication

   This article has 2 authors:

   1. Anup Das
   2. Vinod Menon

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     In this manuscript, the authors present valuable findings on the apparent role of a salience-network anterior insula node in directing fronto-parietal and default-mode network activity within a tripartite network during control of memory, drawn from an impressive invasive human neurophysiological dataset. Overall, the authors have presented a convincing set of analyses. We also commend the use of a large intracranial EEG dataset to approach this question.

   Reviewed by eLife

8. Switching perspective: Comparing ground-level and bird’s-eye views for bumblebees navigating dense environments

   This article has 5 authors:

   1. Annkathrin Sonntag
   2. Odile Sauzet
   3. Mathieu Lihoreau
   4. Martin Egelhaaf
   5. Olivier Bertrand

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     In this useful study, the authors tested the ability of bumblebees to use bird-view and ground-view for homing in cluttered landscapes using modeling and behavioral experiments, claiming that bumblebees rely most on ground-views for homing. However, due to a lack of analysis of the bees' behavior during training and a lack of information as to how the homing behavior of bees develops over time, the evidence supporting their claims is currently incomplete. Moreover, there was concern that the experimental environment was not representative of natural scenes, thus limiting the findings of the study.

   Reviewed by eLife

9. Allosteric modulation by the fatty acid site in the glycosylated SARS-CoV-2 spike

   This article has 10 authors:

   1. A Sofia F Oliveira
   2. Fiona L Kearns
   3. Mia A Rosenfeld
   4. Lorenzo Casalino
   5. Lorenzo Tulli
   6. Imre Berger
   7. Christiane Schaffitzel
   8. Andrew D Davidson
   9. Rommie E Amaro
   10. Adrian J Mulholland

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This manuscript focuses on understanding if and how the glycosylation of SARS-CoV2 spike protein affects a putative allosteric network of interactions controlled by the binding of a fatty acid. The main conclusion is that glycans do not significantly affect the network of allosteric interactions. This valuable information - albeit mainly consisting of negative results - is based on convincing evidence. It will be of interest to scientists focusing on SARS CoV2 protein structure and dynamics.

   Reviewed by eLife

10. Genome-wide mapping of native co-localized G4s and R-loops in living cells

    This article has 8 authors:

    1. Ting Liu
    2. Xing Shen
    3. Yijia Ren
    4. Hongyu Lu
    5. Yu Liu
    6. Chong Chen
    7. Lin Yu
    8. Zhihong Xue

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      This study describes a useful antibody-free method to map both G-quadruplexes and R-loops in vertebrate cells independently of the BG4 and S9.6 antibodies. It also reveals that the helicase Dhx9 can affect the self-renewal and differentiation capacities of mESCs, perhaps by regulating co-localized G4s and R-loops. The datasets provided might constitute a good starting point for future functional studies, and although the strength of the evidence that DHX9 interferes with the ability of mESCs to differentiate by regulating directly the stability of either G4s or R-loops has been improved compared to a previous version, it is still incomplete.

    Reviewed by eLife