eLife

Latest preprint reviews

1. Cell cycle-coupled CK1δ turnover, autoinhibition, and activity

   This article has 4 authors:

   1. Fidel Emmanuel Serrano
   2. Bianca Ruppert
   3. Axel CR Diernfellner
   4. Michael Brunner

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This manuscript presents valuable experimental results describing the localisation and regulation of casein kinase CK1δ during the cell cycle. During the G2 phase of the cell cycle, the autophosphorylation of the C-terminal tail stabilises and inhibits the kinase which might protect CK1δ in the subsequent G1 phase. The results may be of interest to researchers working on casein kinase function and regulation but due to lack of some controls remain incomplete.

     [Editors' note: this paper was reviewed by Review Commons.]

   Reviewed by eLife

2. Life-stage-specific specialities in the cell atlases of the Clytia hemisphaerica planula and medusa

   This article has 14 authors:

   1. Anna Ferraioli
   2. Julia Ramon Mateu
   3. Marc Meynadier
   4. Thomas Lamonerie
   5. Sophie Pagnotta
   6. Sandra Chevalier
   7. Marta Iglesias
   8. Sebastian R Najle
   9. Arnau Sebé-Pedrós
   10. Marie-Jeanne Arguel
   11. Julie Cazareth
   12. Virginie Magnone
   13. Evelyn Houliston
   14. Richard R Copley

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides a useful single-cell atlas of the Clytia hemisphaerica planula, complemented by an updated medusa dataset, ultrastructural analyses and in situ validation of expression patterns. The cross-stage comparison and cluster-similarity framework offer a promising basis for investigating cellular diversification across the life cycle. The evidence is currently incomplete due to documentation deficits and insufficient cross-referencing with prior work. It should be relatively easy to address these issues, which should make this a valued resource for cnidarian researchers and for colleagues studying cell-type evolution.

   Reviewed by eLife

3. Genome-wide architecture of prolonged starvation adaptation in experimentally evolved Drosophila and comparative enrichment in human orthologs

   This article has 6 authors:

   1. Gaurav Yadav
   2. Prachi Mishra
   3. Ranjan Kumar Sahu
   4. Vijendra Sharma
   5. Pawel Michalak
   6. Dau Dayal Aggarwal

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study offers valuable insights into the genetic and evolutionary basis of the starvation response by confirming the hypothesis about the mito-nuclear etiology of this trait. The level of evidence is currently incomplete but could be improved if controls were designed better, particularly by including analysis of variation in the initial, common population prior to all treatments. Overall, this work would be interesting to a broad audience, beyond the Drosophila community, if the analysis of the candidate genes against Human ortholog loci were to be conducted with more careful controls.

   Reviewed by eLife

4. Eco-genomic analysis uncovers precision-conservation targets for the western Pacific’s southernmost salmonid

   This article has 14 authors:

   1. Yi-Chien Lee
   2. Zong-Yu Shen
   3. Wei-Ren Lin
   4. Tzi-Yuan Wang
   5. Mark Liu
   6. Yu-Chen Yeh
   7. Shih-Fan Chan
   8. Mei-Yeh Jade Lu
   9. Hui-Yu Wang
   10. Lin-Yan Liao
   11. Wen-Hsiung Li
   12. Jen-Pan Huang
   13. Isheng Jason Tsai
   14. Sheng-Feng Shen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study combines a chromosome-level genome assembly with population resequencing and demographic modelling to reassess the status of the Formosan landlocked salmon, a critically endangered salmonid at the southern edge of the genus. The assembly and the evidence for extensive lineage-specific chromosomal fusions are genuinely well done, and the discovery of an overlooked, genetically distinct population in Hehuan Creek is sound and of clear management relevance. Evidence for the headline claims is incomplete: species rank is asserted but nowhere argued, and gene flow is excluded using a coalescent model that contains no migration parameter. Support for the population-viability conclusions is also not complete, as the demographic mechanisms invoked in the discussion are not borne out by the supplementary tables.

   Reviewed by eLife

5. The Drosophila IR20a Integrates L-arginine and Salt Signals via Distinct Subunit Assemblies

   This article has 5 authors:

   1. Bo Wang
   2. Guangnan Qu
   3. Xutong Wang
   4. Hubert Amrein
   5. Yan Chen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study uses genetic, behavioural, neuronal activity and cell-based assays to show that the Drosophila ionotropic receptor IR20a contributes to the detection of arginine and low-sodium salt, and suggests that peripheral receptor combinations may help integrate these nutrient cues. The evidence for a role for IR20a and associated co-receptors in these responses is solid, but the evidence that distinct receptor assemblies mediate receptor-level multimodal integration is incomplete, as this central model is inferred from functional data and leaves some discrepancies between behaviour, physiology and prior work unresolved. The study will be of interest to sensory neuroscientists and chemosensory biologists.

   Reviewed by eLife

6. Stimulating neurogenesis of distinct retinal lineages in human retinal pigmented epithelium (RPE) with proneural transcription factors

   This article has 6 authors:

   1. Lew Kaplan
   2. Abigail L Green
   3. Jaz Pang
   4. Marina Pavlou
   5. Juliette Wohlschlegel
   6. Thomas A Reh

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study provides a contribution to retinal regeneration research by using overexpression of pro-neural factors to reprogram fetal human RPE cells into retinal neurons. The authors provide solid evidence of fetal RPE reprogramming into neural and photoreceptor-like states using scRNA-seq and imaging validation. There remains concerns regarding comparisons between the effectiveness of different combinatorial transcription factor codes.

   Reviewed by eLife

7. A nanoscale atlas of extracellular vesicles and particles in Drosophila olfactory sensilla

   This article has 7 authors:

   1. Pawel Vijayakumar
   2. Kalyani Cauwenberghs
   3. Muskaan Garg
   4. Jonathan Choy
   5. Shadi Charara
   6. Quintyn McKaughan
   7. Chih-Ying Su

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study presents an ultrastructural atlas of extracellular vesicles and non-vesicular particles in Drosophila olfactory sensilla, using cryofixation-based serial block-face electron microscopy to catalogue roughly 7,800 particles across four antennal regions. The evidence is compelling: the cryofixation minimizes fixation artifacts, and the rigorous, well-validated methodology and morphometric analyses provide strong support for the structural classifications and spatial distributions. This work provides a foundational resource for researchers studying extracellular-particle signaling in sensory organs.

   Reviewed by eLife

8. Prophase Chromosomes Relocalization to Nuclear Periphery in NPP-3/NUP205 Depletion Protects Genome Stability

   This article has 3 authors:

   1. Ling Jiang
   2. Yu Chung Tse
   3. Karen Wing Yee Yuen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents valuable findings on the functional consequences of nuclear envelope rupture caused by the depletion of nuclear pore complex subunits on the behaviour and organisation of chromosomes during mitosis in C. elegans embryos. The experiments are generally well-designed and executed; however, the evidence for some of the main conclusions is incomplete. This work is of potential interest to cell biologists working on cell division.

   Reviewed by eLife

9. Sequential and coordinated control of human plasma cell differentiation by IRF4 and BLIMP1 associated with a discriminating ISRE/EICE motif lexicon

   This article has 5 authors:

   1. Louis C. Lau
   2. Sneha Lal
   3. Jingyu Fan
   4. Nicholas A. Pease
   5. Harinder Singh

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     In this important study, Lau et al. identify non-conserved nucleotides within the common binding motifs of BLIMP1 and IRF4 that provide a molecular mechanism for their distinct roles as crucial transcription factors during the antibody-secreting cell differentiation. The major strength of this manuscript is the solid and detailed characterization of human in vitro plasma cell differentiation. However, several overstatements exist, therefore requiring careful revision to improve the manuscript.

   Reviewed by eLife

10. Genome-wide mapping of Cas9-induced sister chromatid exchange across single and over 200 genomic sites in human cells

    This article has 3 authors:

    1. Peter Chovanec
    2. Shiyang He
    3. Yi Yin

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      Using sci-L3-Strand-seq, this study shows genome-wide, single-cell evidence that DSBs triggered by CRSIPR/Cas9 are frequently resolved through sister chromatid exchange (SCE), which is undetectable with conventional whole genome sequencing approaches. This important work provides helpful metrics quantifying the occurrence of SCE events at targeted and repetitive genomic loci that are of particular interest to those working in gene editing, DNA repair, genome instability, or repetitive genomic loci. The analysis of reciprocal daughter-cell pairs provides compelling evidence for SCE events, providing evidence consistent with CDK1-TTF2-TRAIP mediated cell-cycle regulated CMG helicase disassembly and fork cleavage at unreplicated regions.

    Reviewed by eLife