eLife

Latest preprint reviews

1. MuSK-BMP signaling in adult muscle stem cells maintains quiescence and regulates myofiber size

   This article has 4 authors:

   1. Laura A Madigan
   2. Diego Jaime
   3. Isabella Chen
   4. Justin R Fallon

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study provides solid support for the participation of the BMP-binding domain of MuSK, a tyrosine kinase mostly known for its role at the neuromuscular junction, in the maintenance and activation of muscle stem cells (SCs). These mononucleated cells, located between the muscle fiber basal lamina and its plasma membrane, are normally quiescent, but following muscle damage, become activated, proliferate, and mediate muscle regeneration. These cells are known to respond to a variety of signaling pathways, but this study makes the case for BMP acting via binding to MuSK in maintaining the quiescent state.

   Reviewed by eLife

2. Ligand-dependent enhancer activation indirectly modulates non-target promoters in a chromatin domain

   This article has 5 authors:

   1. Darshika Bohra
   2. Zubairul Islam
   3. Sundarraj Nidharshan
   4. Aprotim Mazumder
   5. Dimple Notani

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The authors use single molecule imaging and in vivo loop-capture genomic approaches to investigate estrogen mediated enhancer-target gene activation in human cancer cells. Their results, which are supported by solid evidence and will be important for the field, suggest that ER-alpha can, in a temporal delay, activate a non-target gene TFF3, which is in proximity to the main target gene TFF1, through an indirect mechanism as the estrogen responsive enhancer does not loop with the TFF3 promoter. The mechanism of activation may involve condensate formation, however, more future work is needed to fully support a condensate based model. This work will be of interest to those studying transcriptional gene regulation and hormone-aggravated cancers.

   Reviewed by eLife

3. Glucokinase activity controls peripherally located subpopulations of β-cells that lead islet Ca2+ oscillations

   This article has 4 authors:

   1. Erli Jin
   2. Jennifer K Briggs
   3. Richard KP Benninger
   4. Matthew J Merrins

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides compelling evidence for functional subpopulations of β-cells responsible for Ca2+ signal initiation and maintenance using novel three-dimensional light sheet microscopy imaging and analysis of pancreatic islets. The findings are important as they help decode mechanistic underpinnings of islet calcium oscillations and the resulting pulsatile insulin secretion. The work will be of general interest to cell biologists and particular interest to islet biologists.

   Reviewed by eLife

4. The Rab7-Epg5 and Rab39-ema modules cooperatively position autophagosomes for efficient lysosomal fusions

   This article has 10 authors:

   1. Attila Boda
   2. Villő Balázs
   3. Anikó Nagy
   4. Dávid Hargitai
   5. Mónika Lippai
   6. Zsófia Simon-Vecsei
   7. Márton Molnár
   8. Fanni Fürstenhoffer
   9. Gábor Juhász
   10. Péter Lőrincz

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This paper presents valuable findings on how autophagosomes are positioned along microtubules for their efficient fusion with lysosomes, providing significant insights into the mechanism. The evidence supporting the conclusions is solid, with high-quality fluorescence microscopy combined with Drosophila genetics. This work will be of broad interest to cell biologists interested in autophagy and related cell biology fields.

   Reviewed by eLife

5. eIF3 engages with 3’-UTR termini of highly translated mRNAs

   This article has 5 authors:

   1. Santi Mestre-Fos
   2. Lucas Ferguson
   3. Marena I Trinidad
   4. Nicholas T Ingolia
   5. Jamie HD Cate

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study shows previously unappreciated binding of the eukaryotic translation initiation factor 3 (eIF3) to the poly(A) tail proximal portion of 3' untranslated regions (UTRs) of mRNAs that are efficiently translated in neuronal progenitors. The authors' conclusions are supported by solid experimental evidence which is based on several orthogonal systems biology approaches. This article is of considerable interest to the broad spectrum of biomedical researchers interested in studying post-transcriptional regulation of gene expression.

   Reviewed by eLife

6. BEHAV3D Tumor Profiler to map heterogeneous cancer cell behavior in the tumor microenvironment

   This article has 13 authors:

   1. Emilio Rios-Jimenez
   2. Anoek Zomer
   3. Raphael Collot
   4. Mario Barrera Román
   5. Sandra F Archidona
   6. Hendrikus Ariese
   7. Ravian van Ineveld
   8. Michiel Kleinnijenhuis
   9. Nils Bessler
   10. Hannah Johnson
   11. Caleb A Dawson
   12. Anne Rios
   13. Maria Alieva

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This is a useful tool for code-less analysis of patterns in cell migratory behaviours in vivo using intravital microscopy data and allows correlation with spatial features of the tumour microenvironment. There is a clear need for these tools to make quantitative analysis, comparison and interpretation of complex cell tracking data more accessible and solid evidence is provided of its applicability to tracks generated by both proprietary and open tracking software.

   Reviewed by eLife

7. N6-methyladenosine in DNA promotes genome stability

   This article has 16 authors:

   1. Brooke A Conti
   2. Leo Novikov
   3. Deyan Tong
   4. Qing Xiang
   5. Savon Vigil
   6. Thomas J McLellan
   7. Chuong Nguyen
   8. Nancy De La Cruz
   9. Reshma T Veettil
   10. Prashant Pradhan
   11. Parag Sahasrabudhe
   12. Jason D Arroyo
   13. Lei Shang
   14. Benjamin R Sabari
   15. David J Shields
   16. Mariano Oppikofer

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This manuscript reports important findings that the methyltransferase METTL3 is involved in the repair of abasic sites and uracil in DNA, mediating resistance to floxuridine-driven cytotoxicity. The presented evidence is conclusive for the involvement of m6A in DNA involving single cell imaging and mass spectrometry data. The authors present convincing evidence that the m6A signal does not result from bacterial contamination or RNA.

   Reviewed by eLife

8. Mesenchymal Meis2 controls whisker development independently from trigeminal sensory innervation

   This article has 6 authors:

   1. Mehmet Mahsum Kaplan
   2. Erika Hudacova
   3. Miroslav Matejcek
   4. Haneen Tuaima
   5. Jan Křivánek
   6. Ondrej Machon

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides valuable insight into the role of Meis2 in whisker hair follicle formation and confirms prior work that nerves are dispensable for this process. The solid imaging techniques support the authors' conclusions, however the data provides limited evidence to support the mechanism of Meis2 in whisker formation.

   Reviewed by eLife

9. Distinct adaptation and epidemiological success of different genotypes within Salmonella enterica serovar Dublin

   This article has 9 authors:

   1. Cheryll M Sia
   2. Rebecca L Ambrose
   3. Mary Valcanis
   4. Patiyan Andersson
   5. Susan A Ballard
   6. Benjamin P Howden
   7. Deborah A Williamson
   8. Jaclyn S Pearson
   9. Danielle J Ingle

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides the most comprehensive analysis of Salmonella Dublin to date, uncovering distinct genotypic adaptations, antimicrobial resistance patterns, and virulence strategies that influence epidemiological success. The revised manuscript is very valuable, rigorous and compelling.

   Reviewed by eLife

10. Dynamics of compartment-specific proteomic landscapes of hepatotoxic and cholestatic models of liver fibrosis

    This article has 10 authors:

    1. Marketa Jirouskova
    2. Karel Harant
    3. Pavel Cejnar
    4. Srikant Ojha
    5. Katerina Korelova
    6. Lenka Sarnova
    7. Eva Sticova
    8. Christoph H Mayr
    9. Herbert B Schiller
    10. Martin Gregor

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This important study suggests that the composition of the extracellular matrix in a mouse model of liver fibrosis changes depending on the cause of liver fibrosis. The data could be used as a foundation for future antifibrotic therapies. The strength of evidence is convincing with respect to the use of animal models and proteomic analysis. The study provides a helpful inventory of proteins up or down-regulated.

    Reviewed by eLife