eLife

Latest preprint reviews

1. Exercise training at different intensities induces heat stress, disrupts barrier function and alters microbiota in the gut of mice

   This article has 16 authors:

   1. Puqiao Lian
   2. Artemiy Kovynev
   3. Lei Wang
   4. Amanda CM Pronk
   5. Aswin Verhoeven
   6. Martin Giera
   7. Suzan Thijssen
   8. Borja Martínez Téllez
   9. Sander Kooijman
   10. Patrick CN Rensen
   11. Harro Timmerman
   12. Harry J Wichers
   13. Paul AJ Henricks
   14. Gert Folkerts
   15. Milena Schönke
   16. Saskia Braber

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study examines how different exercise training intensities affect intestinal barrier function and gut microbiota composition over a 6-week period in mice. The evidence supporting the main claims about exercise-induced intestinal injury and microbiota changes is solid, featuring comprehensive histological analysis, molecular characterization, and metabolomic profiling, though key mechanistic insights and causal relationships remain to be established. The findings have practical implications for understanding exercise-induced gastrointestinal stress, particularly the observation that daily moderate exercise may be more damaging to intestinal integrity than vigorous exercise with rest days. Additional experimental validation would strengthen these conclusions.

   Reviewed by eLife

2. Synaptic connectome of a neurosecretory network in the Drosophila brain

   This article has 8 authors:

   1. Theresa H McKim
   2. Jayati Gera
   3. Ariana J Gayban
   4. Nils Reinhard
   5. Giulia Manoli
   6. Selina Hilpert
   7. Charlotte Helfrich-Förster
   8. Meet Zandawala

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study uses the analysis of connectomic and transcriptomic datasets to survey the anatomy and connectivity of neurosecretory cells in the Drosophila brain. While the connectivity analyses are convincing, the anatomical and functional data provided to verify cell type identity and paracrine signaling is incomplete. Once these aspects are improved, this study would be of interest to neuroscientists working on hormonal signaling in Drosophila and other animals.

   Reviewed by eLife

3. Temporal dynamics of viral fitness and the adaptive immune response in HCV infection

   This article has 7 authors:

   1. Melanie Rose Walker
   2. Preston Leung
   3. Elizabeth Keoshkerian
   4. Mehdi R Pirozyan
   5. Andrew Lloyd
   6. Fabio Luciani
   7. Rowena A Bull

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The authors examined the evolution of hepatitis C virus (HCV) in a cohort of 14 subjects with recent HCV infections. They showed that viral fitness declines as the virus mutates to escape the immune response and can rebound later in infection as HCV accumulates additional mutations. The study contributes to an important aspect of viral evolution. The combination of approaches contributes to a convincing study.

   Reviewed by eLife

4. Inhibition on neutrophil extracellular traps by oligomeric procyanidins alleviate chemotherapy-induced chronic kidney injury via gut-kidney axis

   This article has 11 authors:

   1. Yaqi Luan
   2. Weiwei He
   3. Kunmao Jiang
   4. Shenghui Qiu
   5. Lan Jin
   6. Xinrui Mao
   7. Ying Huang
   8. Wentao Liu
   9. Jingyuan Cao
   10. Lai Jin
   11. Rong Wang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study provides evidence for the role of neutrophil extracellular traps in chronic kidney damage (CKD) induced by chemotherapy and suggests a therapeutic approach to mitigate the kidney pathology caused by the NETs. The study utilizes a sound murine in vivo model of CKD with low-dose administration cisplatin and a genetic model for impairment of NET formation by deletion of the enzyme Pad4. In its current form, the study was seen as incomplete as there is not yet formal demonstration of NET production by neutrophils in the model of CKD used. Additionally, the accuracy and clarity of data presentation could be improved.

   Reviewed by eLife

5. Diversity and functional specialization of oyster immune cells uncovered by integrative single-cell level investigations

   This article has 9 authors:

   1. Sebastien De La Forest Divonne
   2. Juliette Pouzadoux
   3. Oceane Romatif
   4. Caroline Montagnani
   5. Guillaume Mitta
   6. Delphine Destoumieux-Garzón
   7. Benjamin Gourbal
   8. Guillaume M Charriere
   9. Emmanuel Vignal

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The manuscript by de La Forest Divonne et al. offers an important and detailed exploration of the immune cells in the oyster Crassostrea gigas, by correlating distinct hemocyte morphotypes with specific single-cell transcriptional profiles. The evidence supporting the conclusion is convincing, deriving from the comprehensive dataset that not only captures unicellular diversity but also associates these cells with distinct immune roles, making it an invaluable resource for the broader research community.

   Reviewed by eLife

6. TRPV3 channel activity helps cortical neurons stay active during fever

   This article has 5 authors:

   1. Yiming Shen
   2. Richárd Fiáth
   3. Baskar Mohana Krishnan
   4. István Ulbert
   5. Michelle W Antoine

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This is a valuable study of the physiological mechanisms promoting network activity during fever in the mouse neocortex. The supporting evidence is solid, and has improved with revision, along with increased clarity of presentation.

   Reviewed by eLife

7. The promise and peril of comparing fluorescence lifetime in biology revealed by simulations

   This article has 4 authors:

   1. Pingchuan Ma
   2. Peter Chen
   3. Scott Sternson
   4. Yao Chen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents an important computational framework, FLiSimBA (Fluorescence Lifetime Simulation for Biological Applications), for modeling experimental limitations in Fluorescence Lifetime Imaging Microscopy (FLIM). FLiSimBA is readily available in MATLAB and Python, enables users to simulate effects of noise and varying sensor expression levels, and provides practical guidance for both lifetime imaging experiments and biosensor development. The analyses are robust, and the evidence supporting the tool's utility in distinguishing between multiple lifetime signals is compelling, indicating strong potential for multiplexed dynamic imaging. However, users should also consider that the tool's effectiveness depends on the suitability of a two-component discrete exponential model.

   Reviewed by eLife

8. Realistic mossy fiber input patterns to unipolar brush cells evoke a continuum of temporal responses comprised of components mediated by different glutamate receptors

   This article has 2 authors:

   1. Vincent Huson
   2. Wade G Regehr

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study describes how trains of mossy fiber stimulation control cerebellar unipolar brush cell discharges. The dissection of the contributions of relevant glutamate receptors to these transformations is convincing. Overall, the study broadens our understanding of temporal processing in the cerebellar cortex.

   Reviewed by eLife

9. Mistargeted retinal axons induce a synaptically independent subcircuit in the visual thalamus of albino mice

   This article has 7 authors:

   1. Sean McCracken
   2. Liam McCoy
   3. Ziyi Hu
   4. Julie A Hodges
   5. Katia Valkova
   6. Philip R Williams
   7. Josh L Morgan

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides important observations about the role of Hebbian synapse rewiring (which predicts that correlated activity between neurons begets stronger synapses) on brain connectivity development, by examining a naturally emerging case where Hebbian predictions can be tested because neurons with differing activity undergo development under otherwise similar conditions (albino mouse lateral geniculate nucleus [LGN], where retinal ganglion cells [RGCs] from the contralateral retina form inappropriate projections alongside a majority of ipsilateral RGCs). The evidence supporting the conclusions is compelling, with combined confocal imaging and serial electron microscopy (EM) reconstructions showing complete synaptic isolation of aberrantly projecting RGCs onto LGN thalamocortical projection neurons, and mixed connectivity onto LGN local interneurons. The morphological descriptions of connectivity presented here will be of interest to researchers studying synaptic connectivity and plasticity during development.

   Reviewed by eLife

10. Lacticaseibacillus rhamnosus P118 enhances host tolerance to Salmonella infection by promoting microbe-derived indole metabolites

    This article has 10 authors:

    1. Baikui Wang
    2. Xianqi Peng
    3. Xiao Zhou
    4. Xiuyan Jin
    5. Abubakar Siddique
    6. Jiayun Yao
    7. Haiqi Zhang
    8. Weifen Li
    9. Yan Li
    10. Min Yue

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      The microbiome field is constantly providing insight on various health-related properties elicited by the commensals that inhabit their mammalian hosts. Harnessing the potential of these commensals for knowledge about host-microbe interactions, as well as properties with therapeutic implications, will likely remain a fruitful field for decades to come. In this valuable study, Wang et al use various methods, encompassing classic microbiology, genomics, chemical biology, and immunology, to identify a potent probiotic strain that protects nematode and murine hosts from Salmonella enterica infection. The authors provide compelling evidence identifying gut metabolites that are correlated with protection, and show that a single metabolite can recapitulate the effects of probiotic administration.

    Reviewed by eLife