eLife

Latest preprint reviews

1. A macroevolution-inspired approach to reveal novel antibiotic resistance mechanisms

   This article has 7 authors:

   1. Fernanda T Subtil
   2. Teresa FG Machado
   3. Holly Douglas
   4. Joanna M Kirkpatrick
   5. Mark Skehel
   6. Acely Garza-Garcia
   7. Luiz Pedro S de Carvalho

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study introduces an approach to discovering antibiotic resistance determinants by leveraging diverse susceptibility profiles among related mycobacterial species, with particular relevance to high-level resistance against natural product-derived antibiotics. The research provides convincing evidence for the role of ADP-ribosylation enzymes in rifamycin resistance among mycobacteria, whilst also demonstrating that antibiotic susceptibility is not correlated with growth rate or intracellular compound concentration. Although some broader claims require additional experimental support, this work lays a significant foundation for understanding the complexity of antibiotic resistance mechanisms in mycobacteria and opens new avenues for future antimicrobial research.

   Reviewed by eLife

2. Coordinated Tbx3/Tbx5 transcriptional control of the adult ventricular conduction system

   This article has 13 authors:

   1. Ozanna Burnicka-Turek
   2. Katy A Trampel
   3. Brigitte Laforest
   4. Michael T Broman
   5. Xinan H Yang
   6. Zoheb Khan
   7. Eric Rytkin
   8. Binjie Li
   9. Ella Schaffer
   10. Margaret Gadek
   11. Kaitlyn M Shen
   12. Igor R Efimov
   13. Ivan P Moskowitz

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The work presented is important for our understanding of the development of the cardiac conduction system and its regulation by T-box transcription factors. The conclusions are supported by convincing data. Overall this is an excellent study that advances our understanding of cardiac biology and has implications beyond the immediate field of study.

   Reviewed by eLife

3. Adaptive Integration of Perceptual and Reward Information in an Uncertain World

   This article has 5 authors:

   1. Prashanti Ganesh
   2. Radoslaw M Cichy
   3. Nicolas W Schuck
   4. Carsten Finke
   5. Rasmus Bruckner

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study by Ganesh and colleagues examined how both the value and salience of sensory information can affect economic decision-making. The results provide insights into how different sources of uncertainty found in the real world, including those related to the perception of objects and those related to values associated with objects, can together influence decision-making behavior in systematic ways. The evidence is solid but overlaps with previous studies and could be improved by clarifying novelty and experimental details and considering additional models.

   Reviewed by eLife

4. Competition for the conserved branch point sequence influences physiological outcomes in pre-mRNA splicing

   This article has 7 authors:

   1. Karen Larisssa Pereira de Castro
   2. Jose M Abril
   3. Kuo-Chieh Liao
   4. Haiping Hao
   5. John Paul Donohue
   6. William K Russell
   7. William S Fagg

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important manuscript provides insights into the competition between Splicing Factor 1 (SF1) and Quaking (QKI) for binding at the ACUAA branch point sequence in a model intron, regulating exon inclusion. The study employs convincing, rigorous transcriptomic, proteomic, and reporter assays, with both mammalian cell culture and yeast models.

   Reviewed by eLife

5. The Shigella flexneri effector IpaH1.4 facilitates RNF213 degradation and protects cytosolic bacteria against interferon-induced ubiquitylation

   This article has 9 authors:

   1. Luz Saavedra-Sanchez
   2. Mary S Dickinson
   3. Shruti S Apte
   4. Yifeng Zhang
   5. Maarten De Jong
   6. Samantha Skavicus
   7. Nicholas S Heaton
   8. Neal M Alto
   9. Jorn Coers

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     In this manuscript, the authors report the fundamental finding that a secreted ubiquitin ligase of Shigella, called IpaH1.4, mediates the degradation of a host defense factor, RNF213. The data are convincing and represent a major contribution to our understanding of cell-autonomous immunity and bacterial pathogenesis as they provide new mechanistic insight into how the cytosolic bacterial pathogen Shigella flexneri evades IFN-induced host immunity.

   Reviewed by eLife

6. MuSK-BMP signaling in adult muscle stem cells maintains quiescence and regulates myofiber size

   This article has 4 authors:

   1. Laura A Madigan
   2. Diego Jaime
   3. Isabella Chen
   4. Justin R Fallon

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study provides solid support for the participation of the BMP-binding domain of MuSK, a tyrosine kinase mostly known for its role at the neuromuscular junction, in the maintenance and activation of muscle stem cells (SCs). These mononucleated cells, located between the muscle fiber basal lamina and its plasma membrane, are normally quiescent, but following muscle damage, become activated, proliferate, and mediate muscle regeneration. These cells are known to respond to a variety of signaling pathways, but this study makes the case for BMP acting via binding to MuSK in maintaining the quiescent state.

   Reviewed by eLife

7. Acute Activation of Genes Through Transcriptional Condensates Impact Non-target Genes in a Chromatin Domain

   This article has 5 authors:

   1. Darshika Bohra
   2. Zubairul Islam
   3. Sundarraj Nidharshan
   4. Aprotim Mazumder
   5. Dimple Notani

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The authors use single molecule imaging and in vivo loop-capture genomic approaches to investigate estrogen mediated enhancer-target gene activation in human cancer cells. These potentially important results suggest that ER-alpha can, in a temporal delay, activate a non-target gene TFF3, which is in proximity to the main target gene TFF1, even though the estrogen responsive enhancer does not loop with the TFF3 promoter. To explain these results, the authors invoke a transcriptional condensate model. The claim of a temporal delay and effects of the target gene transcription on the non-target gene expression are supported by solid evidence but there is no direct evidence of the role of a condensate in mediating this effect. The reviewers appreciate that the authors have done a lot of work to strengthen the study. This work will be of interest to those studying transcriptional gene regulation and hormone-aggravated cancers.

   Reviewed by eLife

8. Glucokinase activity controls peripherally located subpopulations of β-cells that lead islet Ca2+ oscillations

   This article has 4 authors:

   1. Erli Jin
   2. Jennifer K Briggs
   3. Richard KP Benninger
   4. Matthew J Merrins

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides compelling evidence for functional subpopulations of β-cells responsible for Ca2+ signal initiation and maintenance using novel three-dimensional light sheet microscopy imaging and analysis of pancreatic islets. The findings are important as they help decode mechanistic underpinnings of islet calcium oscillations and the resulting pulsatile insulin secretion. The work will be of general interest to cell biologists and particular interest to islet biologists.

   Reviewed by eLife

9. The Rab7-Epg5 and Rab39-ema modules cooperatively position autophagosomes for efficient lysosomal fusions

   This article has 10 authors:

   1. Attila Boda
   2. Villő Balázs
   3. Anikó Nagy
   4. Dávid Hargitai
   5. Mónika Lippai
   6. Zsófia Simon-Vecsei
   7. Márton Molnár
   8. Fanni Fürstenhoffer
   9. Gábor Juhász
   10. Péter Lőrincz

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This paper presents valuable findings on how autophagosomes are positioned along microtubules for their efficient fusion with lysosomes, providing significant insights into the mechanism. The evidence supporting the conclusions is solid, with high-quality fluorescence microscopy combined with Drosophila genetics. This work will be of broad interest to cell biologists interested in autophagy and related cell biology fields.

   Reviewed by eLife

10. eIF3 engages with 3’-UTR termini of highly translated mRNAs

    This article has 5 authors:

    1. Santi Mestre-Fos
    2. Lucas Ferguson
    3. Marena I Trinidad
    4. Nicholas T Ingolia
    5. Jamie HD Cate

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This valuable study shows previously unappreciated binding of the eukaryotic translation initiation factor 3 (eIF3) to the poly(A) tail proximal portion of 3' untranslated regions (UTRs) of mRNAs that are efficiently translated in neuronal progenitors. The authors' conclusions are supported by solid experimental evidence which is based on several orthogonal systems biology approaches. This article is of considerable interest to the broad spectrum of biomedical researchers interested in studying post-transcriptional regulation of gene expression.

    Reviewed by eLife