eLife

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1. We couldn't get details of this article at this time:
   10.1101/2025.03.03.641205

2. We couldn't get details of this article at this time:
   10.1101/2025.04.14.648691

3. Temperature-based MHC class-I multimer peptide exchange for human HLA-A, B and C

   This article has 13 authors:

   1. Cilia R Pothast
   2. Ian Derksen
   3. Anneloes van der Plas - van Duijn
   4. Angela el Hebieshy
   5. Wesley Huisman
   6. Kees LMC Franken
   7. Jacques Neefjes
   8. Jolien J Luimstra
   9. Marieke Griffioen
   10. Michel Kester
   11. Maarten H Vermeer
   12. Mirjam HM Heemskerk
   13. Ferenc A Scheeren

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents an alternative to conventional and UV-based tetramers, which are easy to use and reliable for the identification of antigen-specific CD8 T cells. The authors demonstrate that tetramers for HLA alleles A0301, A1101, B0702, and C0702 can be subjected to specific temperatures that facilitate peptide exchange, whilst maintaining structural integrity. Whilst the strength of the evidence is currently incomplete, further development and validation of this approach is likely to provide a useful alternative to generating reagents for examining T cell specificities.

   Reviewed by eLife

4. Type I interferon responses contribute to immune protection against mycobacterial infection

   This article has 14 authors:

   1. Andrea Szydlo-Shein
   2. Blanca Sanz-Magallón Duque de Estrada
   3. Joshua Rosenheim
   4. Carolin T Turner
   5. Aneesh Chandran
   6. Evdokia Tsaliki
   7. Marc CI Lipman
   8. Heinke Kunst
   9. Gabriele Pollara
   10. Philip M Elks
   11. Jean-Pierre Levraud
   12. Elspeth M Payne
   13. Mahdad Noursadeghi
   14. Gillian S Tomlinson

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This useful study shows a protective role of type 1 IFN during Mycobacterium tuberculosis infection. It shows that the type 1 IFN response in human skin TST inversely correlates with TB severity, suggesting its protective role. Considering that type I IFN is usually shown to be pro-pathogenic, the higher vulnerability of zebrafish larvae lacking stat2 to M marinum infection is a strong result. However, the conclusion that IFN-I is protective during mycobacterial infection remains indirect and incomplete; the study requires additional mechanistic insights and validation.

   Reviewed by eLife

5. Synonymous mutations in AAV Rep enhance genome packaging in a library selection

   This article has 3 authors:

   1. Tasfia Azim
   2. Dru Myerscough
   3. Jonathan J Silberg

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     Engineering of adeno-associated virus (AAV) replication proteins may provide new insights into Parvoviral replication. The authors created a useful collection of Rep protein variants with changes that alter the amino acid sequence, but these did not lead to clear improvements in how the virus worked. Instead, their screen showed that changes that do not alter the protein ("synonymous" mutations) and changes to the promoter were more common. As it stands the results are incomplete due to potential issues with the screening design. We encourage a more complete characterization, which may enhance the translational potential of the approach.

   Reviewed by eLife

6. We couldn't get details of this article at this time:
   10.1101/2025.04.11.648449

7. We couldn't get details of this article at this time:
   10.1101/2025.04.03.647082

8. We couldn't get details of this article at this time:
   10.1101/2024.10.24.619578

9. We couldn't get details of this article at this time:
   10.1101/2024.03.13.584141

10. Membrane curvature regulates Ups1 dependent phosphatidic acid transfer across lipid bilayers

    This article has 10 authors:

    1. Fereshteh Sadeqi
    2. Dexin Dong
    3. Kai Stroh
    4. Marian Vache
    5. Jutta Metz
    6. Dietmar Riedel
    7. Andreas Janshoff
    8. Herre Jelger Risselada
    9. Caroline Kolenda
    10. Michael Meinecke

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This fundamental study combines in vitro reconstitution experiments and molecular dynamics simulations to elucidate how membrane lipids are transported from the outer to the inner membrane of mitochondria. The authors provide convincing evidence that a positive membrane curvature is critical for membrane lipid extraction. The work will be of broad interest to cell biologists and biochemists.

    Reviewed by eLife