eLife

Latest preprint reviews

1. Multi-omics investigation of spontaneous T2DM macaque emphasizes gut microbiota could up-regulate the absorption of excess palmitic acid in the T2DM progression

   This article has 13 authors:

   1. Xu Liu
   2. Yuchen Xie
   3. Shengzhi Yang
   4. Cong Jiang
   5. Ke Shang
   6. Jinxia Luo
   7. Lin Zhang
   8. Gang Hu
   9. Qinghua Liu
   10. Bisong Yue
   11. Zhenxin Fan
   12. Zhanlong He
   13. Jing Li

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important work substantially advances our understanding of the interaction among gut microbiota, lipid metabolism, and the host in type 2 diabetes. The evidence supporting the claims of the authors is convincing. The work will be of interest to medical biologists working on microbiota and diabetes.

   Reviewed by eLife

2. Interpretable protein-DNA interactions captured by structure-sequence optimization

   This article has 4 authors:

   1. Yafan Zhang
   2. Irene Silvernail
   3. Zhuyang Lin
   4. Xingcheng Lin

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable work presents an interpretable protein-DNA Energy Associative (IDEA) model for predicting binding sites and affinities of DNA-binding proteins. While the method is convincing, it requires some adaptation for application to different proteins. The IDEA method is available and can be potentially used for predicting genome-wide protein-DNA binding sites.

   Reviewed by eLife

3. Increased reluctant vesicles underlie synaptic depression by GPR55 in axon terminals of rat cerebellar Purkinje cells

   This article has 2 authors:

   1. Takuma Inoshita
   2. Shin-ya Kawaguchi

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This is an important study reporting that activation of the presynaptic GPR55 receptor suppresses synaptic transmission by modulating GABA release through the reduction of the readily releasable pool without affecting the presynaptic AP waveform and calcium influx. The evidence supporting this claim is compelling and based on an impressive array of techniques including patch-clamp recordings from the axon terminals of cerebellar Purkinje cells and fluorescent imaging of vesicular exocytosis. While the authors have strengthened their conclusions on several technical fronts in the revised version, further investigation is needed into the mechanism by which GPR55 activation might make vesicles insensitive to the rise in presynaptic [Ca²⁺] mediated by VGCCs, and the nature of the endogenous process that would activate this pathway in vivo.

   Reviewed by eLife

4. Targeting SLC7A11-mediated cysteine metabolism for the treatment of trastuzumab-resistant HER2-positive breast cancer

   This article has 14 authors:

   1. Yijia Hua
   2. Ningjun Duan
   3. Chunxiao Sun
   4. Fan Yang
   5. Min Tian
   6. Yanting Sun
   7. Shuhan Zhao
   8. Jue Gong
   9. Qian Liu
   10. Xiang Huang
   11. Yan Liang
   12. Ziyi Fu
   13. Wei Li
   14. Yongmei Yin

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides compelling evidence that SLC7A11 may serve as a potential therapeutic target for trastuzumab-resistant HER2-positive breast cancer. While the findings are well-supported by robust data, the study could have been further strengthened by incorporating additional cell line experiments and providing more detailed clarification on patient sample selection. Nevertheless, this valuable work represents a significant contribution and will be of considerable interest to researchers in the field of breast cancer.

   Reviewed by eLife

5. Combined transcriptomic, connectivity, and activity profiling of the medial amygdala using highly amplified multiplexed in situ hybridization (hamFISH)

   This article has 15 authors:

   1. Mathew D Edwards
   2. Ziwei Yin
   3. Risa Sueda
   4. Alina Gubanova
   5. Chang S Xu
   6. Virág Lakner
   7. Megan Murchie
   8. Chi-Yu Lee
   9. Kristal Ng
   10. Karolina Farrell
   11. Rupert Faraway
   12. Subham Ganguly
   13. Elina Jacobs
   14. Bogdan Bintu
   15. Yoh Isogai

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The study presents important findings that are highly relevant for research aiming to combine transcriptomics, connectivity studies, and activity profiling in the rodent brain and the revisions improve the study. The evidence overall remains convincing as the authors use appropriate and validated methodology in line with current state-of-the-art.

   Reviewed by eLife

6. Complex system modeling reveals oxalate homeostasis is driven by diverse oxalate-degrading bacteria

   This article has 11 authors:

   1. Sromona D Mukherjee
   2. Carlos Batagello
   3. Ava Adler
   4. Jose Agudelo
   5. Anna Zampini
   6. Mangesh Suryavanshi
   7. Andrew Nguyen
   8. Terry Orr
   9. Denise Dearing
   10. Manoj Monga
   11. Aaron W Miller

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This work presents a valuable approach based on a complex systems theoretical framework to characterize diet-host-microbe interactions and develop targeted bacteriotherapies through a three-phase workflow. Despite the partial support of the description and experimental setup of the 'complex systems theoretical approach,' the collected data are solid and advance our understanding of oxalate bacterial metabolism in microbial communities. This study will interest researchers working on gut microbiomes and the possible modulation of host-microbial interactions.

   Reviewed by eLife

7. Serial ‘deep-sampling’ PCR of fragmented DNA reveals the wide range of Trypanosoma cruzi burden among chronically infected human, macaque, and canine hosts, and allows accurate monitoring of parasite load following treatment

   This article has 7 authors:

   1. Brooke E White
   2. Carolyn L Hodo
   3. Sarah Hamer
   4. Ashley B Saunders
   5. Susana A Laucella
   6. Daniel B Hall
   7. Rick L Tarleton

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents an important methodological advance to improve the sensitivity of PCR for detecting Trypanosoma cruzi in blood, combining DNA fragmentation, deep sampling, and blood cell pellet analysis. The findings offer solid evidence of enhanced detection sensitivity and shed light on parasite load dynamics during chronic infection in mammalian reservoirs. The evidence is sound for macaques and the method shows promise in expanding detection limits, but there is some variability in the limits of detection and small sample size of human samples. This work will be of interest to parasitologists, epidemiologists, and clinicians using molecular diagnostics to monitor responses to etiological treatments for Chagas disease.

   Reviewed by eLife

8. Timely vaccine strain selection and genomic surveillance improve evolutionary forecast accuracy of seasonal influenza A/H3N2

   This article has 2 authors:

   1. John Huddleston
   2. Trevor Bedford

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study investigates the influence of genomic information and timing of vaccine strain selection on the accuracy of influenza A/H3N2 forecasting. The authors utilised appropriate statistical methods and have provided convincing evidence, which amounts to an important contribution to the evidence base. Substantial revisions have been made to the manuscript and issues of concern have been clarified, with the necessary study limitations appropriately discussed.

   Reviewed by eLife

9. HERV activation segregates ME/CFS from fibromyalgia while defining a novel nosologic entity

   This article has 4 authors:

   1. Karen Giménez-Orenga
   2. Eva Martín-Martínez
   3. Lubov Nathanson
   4. Elisa Oltra

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study substantially expands observations of HERV expression in the clinical settings. The evidence provided by the authors that HERV activity is an underlying etiological factor in ME/CFS and fibromyalgia is compelling and suggests further investigation into mechanisms. This work will be of broad interest to clinicians and researchers alike.

   Reviewed by eLife

10. RAS–p110α signalling in macrophages is required for effective inflammatory response and resolution of inflammation

    This article has 19 authors:

    1. Alejandro Rosell
    2. Agata Adelajda Krygowska
    3. Marta Alcón Pérez
    4. Cristina Cuesta
    5. Mathieu-Benoit Voisin
    6. Juan de Paz
    7. Héctor Sanz-Fraile
    8. Vinothini Rajeeve
    9. Ana Carreras-González
    10. Alberto Berral-González
    11. Ottilie Swinyard
    12. Enrique Gabandé-Rodríguez
    13. Julian Downward
    14. Jordi Alcaraz
    15. Juan Anguita
    16. Carmen García-Macías
    17. Javier De Las Rivas
    18. Pedro R Cutillas
    19. Esther Castellano Sanchez

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This useful study investigates the impact of disrupting the interaction of RAS with the PI3K subunit p110α in macrophage function in vitro and inflammatory responses in vivo. Solid data overall supports a role for RAS-p110α signalling in regulating macrophage activity and so inflammation, however for many of the readouts presented the magnitude of the phenotype is not particularly pronounced. Further analysis would be required to substantiate the claims that RAS-p110α signalling plays a key role in macrophage function. Of note, the molecular mechanisms of how exactly p110α regulates the functions in macrophages have not yet been established.

    Reviewed by eLife