eLife

Latest preprint reviews

1. Observation of persister cell histories reveals diverse modes of survival in antibiotic persistence

   This article has 8 authors:

   1. Miki Umetani
   2. Miho Fujisawa
   3. Reiko Okura
   4. Takashi Nozoe
   5. Shoichi Suenaga
   6. Hidenori Nakaoka
   7. Edo Kussell
   8. Yuichi Wakamoto

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The work is interesting in its characterization of a large number of antibiotic persisters from a wild-type strain. Previous work was typically limited to directly observe either high persister strains or a smaller number of wt persisters. Therefore, it sheds new light on the elusive non-dormant persisters present in exponentially growing cultures and should help resolve previous conflicting observations.

   Reviewed by eLife

2. Nora virus proliferates in dividing intestinal stem cells and thereby sensitizes Drosophila flies to Pseudomonas aeruginosa intestinal infection and to oxidative stress

   This article has 12 authors:

   1. Adrien Franchet
   2. Samantha Haller
   3. Miriam Yamba
   4. Vincent Barbier
   5. Angelica Thomaz-Vieira
   6. Vincent Leclerc
   7. Stefanie Becker
   8. Kwang-Zin Lee
   9. Igor Orlov
   10. Danièle Spehner
   11. Laurent Daeffler
   12. Dominique Ferrandon

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study shows that the Nora virus, a natural Drosophila pathogen that also persistently infects many laboratory fly stocks, infects intestinal stem cells (ISCs), leading to a shorter life span and increased sensitivity to intestinal infection with the bacterium Pseudomonas. The authors provide convincing data to support their conclusions. The paper provides new insights into virus-host interactions in the Drosophila gut and serves as a warning for scientists who use the fruit fly as a model to study gut physiology.

   Reviewed by eLife

3. Sense of control buffers against stress

   This article has 5 authors:

   1. Jennifer C Fielder
   2. Jinyu Shi
   3. Daniel McGlade
   4. Quentin JM Huys
   5. Nikolaus Steinbeis

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important research addresses the effects of subjective control and task difficulty on experienced stress using a novel behavioral task administered on the same day in two large online samples. Convincing evidence is provided, establishing the internal and external task validity of the task, as well as a relationship between the sense of control and task difficulty, with individual differences in relevant mental health constructs. Evidence for the specificity of the link between control and stress would be more substantial if the design had not conflated control and reward rate. This work will be of interest to psychologists and clinicians studying the concepts of controllability, stress, and psychopathology.

   Reviewed by eLife

4. The mechanism of DRB7.2:DRB4 mediated sequestering of endogenous inverted-repeat dsRNA precursors in plants

   This article has 7 authors:

   1. Sneha Paturi
   2. Debadutta Patra
   3. Priti Chanda Behera
   4. Ramdas Aute
   5. Nilam Waghela
   6. Priyadarshan Kinatukara
   7. Mandar V Deshmukh

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The manuscript provides valuable findings in the field for understanding the RNAi regulation in plants at the molecular level with a model of how DRB7.2 and DRB4 form a heterodimer and protect dsRNA from DICER activity. The presented data provide a solid basis for the model, but certain measurements could benefit from replicates for robust statistics.

   Reviewed by eLife

5. Navigating contradictions in enteric chemotactic stimuli

   This article has 5 authors:

   1. Kailie Franco
   2. Zealon Gentry-Lear
   3. Michael Shavlik
   4. Michael J Harms
   5. Arden Baylink

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     In this manuscript, Franco and colleagues present compelling evidence that fecal extracts containing high concentrations of indole, a known repellent, enhance rather than protect against invasion of colonic tissue by Salmonella. The authors describe important findings that lead to the conclusion that the competing effects of attractants present in fecal matter, including L-serine, also sensed by the Tsr chemoreceptor that senses indole, override the repulsive effect of indole.

   Reviewed by eLife

6. C-terminal tagging, transmembrane domain hydrophobicity, and an ER retention motif influence the secretory trafficking of the inner nuclear membrane protein emerin

   This article has 4 authors:

   1. Jessica Mella
   2. Regan F Volk
   3. Balyn W Zaro
   4. Abigail Buchwalter

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study presents a valuable finding on the delivery of a nuclear envelop protein to lysosomes and the impact of C-terminal tagging on its traffic. The authors provide solid evidence for the potential artifacts introduced by large terminal tags, particularly in the context of membrane protein localization and stability.

   Reviewed by eLife

7. Agent-based modeling reveals how bats navigate dense group emergences

   This article has 2 authors:

   1. Omer Mazar
   2. Yossi Yovel

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important model-based study seeks to mimic bat echolocation behavior and flight under conditions of high interference, such as when large numbers of bats leave their roost together. The simulations convincingly suggest that the problem of acoustic jamming in these situations may be less severe than previously thought. This finding will be of broad interest to scientists working in the fields of bat biology and collective behaviour.

   Reviewed by eLife

8. MARK2 regulates Golgi apparatus reorientation by phosphorylation of CAMSAP2 in directional cell migratio

   This article has 11 authors:

   1. Peipei Xu
   2. Rui Zhang
   3. Zhengrong Zhou
   4. Honglin Xu
   5. Yuejia Li
   6. Mengge Yang
   7. Ruifan Lin
   8. Yingchun Wang
   9. Xiahe Huang
   10. Qi Xie
   11. Wenxiang Meng

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The authors propose that the kinase MARK2 regulates the Golgi's reorientation towards the cell's leading edge through the regulation of microtubule binding protein CAMSAP2 and its binding to USO1. While the model is interesting and the study is useful, the quantification of an insufficient number of cells and insufficient description of the methods and biological replicates mean the results are inadequate to support the model.

     [Editors' note: this paper was reviewed by Review Commons.]

   Reviewed by eLife

9. Kinetic regulation of kinesin’s two motor domains coordinates its stepping along microtubules

   This article has 5 authors:

   1. Yamato Niitani
   2. Kohei Matsuzaki
   3. Erik Jonsson
   4. Ronald D Vale
   5. Michio Tomishige

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study provides compelling evidence that kinesin's stepping mechanism is governed by strain-induced conformational changes in its nucleotide-binding pockets. Using pre-steady state kinetics and single-molecule assays, the authors demonstrate that the neck linker's conformation differentially modulates nucleotide affinity and detachment rates, establishing an asynchronous chemo-mechanical cycle that prevents simultaneous detachment. Supported by cryo-EM structural data, the work presents an important advance in our understanding of kinesin's hand-over-hand movement.

     [Editors' note: this paper was reviewed by Review Commons.]

   Reviewed by eLife, Review Commons

10. The title and authors for this article are not available from our external data provider yet:
    10.48550/arxiv.2303.14159v8