eLife

Latest preprint reviews

1. CRISPR-edited DPSCs constitutively expressing BDNF enhance dentin regeneration in injured teeth

   This article has 6 authors:

   1. Ji Hyun Kim
   2. Muhammad Irfan
   3. Sreelekshmi Sreekumar
   4. Atsawasuwan Phimon
   5. Stephanie Kim
   6. Seung Chung

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study on the effect of the trophic factor BDNF upon dental cells is an understudied subject that is relevant to dental regeneration and repair. Given that the topic is new and has not been covered previously, the report is a useful foray into a new area of investigation, although several experimental results could be strengthened. The connection of BDNF and dental health is a solid attempt in potentially translating trophic factor signaling clinically, which has been stymied in past efforts.

   Reviewed by eLife

2. Molecular architecture of thylakoid membranes within intact spinach chloroplasts

   This article has 8 authors:

   1. Wojciech Wietrzynski
   2. Lorenz Lamm
   3. William HJ Wood
   4. Matina-Jasemi Loukeri
   5. Lorna Malone
   6. Tingying Peng
   7. Matthew P Johnson
   8. Benjamin D Engel

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The macromolecular organization of photosynthetic complexes within the thylakoids of higher plant chloroplasts has been a topic of significant debate. Using in situ cryo-electron tomography, this study reveals the native thylakoid architecture of spinach thylakoid membranes with single-molecule precision. The experimental methods are unique and compelling, providing important information for understanding the structural features that impact photosynthetic regulation in vascular plants and addressing several long-standing questions about the organization and regulation of photosynthesis.

   Reviewed by eLife

3. Bcl11b orchestrates subcerebral projection neuron axon development via cell-autonomous, non-cell-autonomous, and subcellular mechanisms

   This article has 7 authors:

   1. Yasuhiro Itoh
   2. Mollie B Woodworth
   3. Luciano C Greig
   4. Anne K Engmann
   5. Dustin E Tillman
   6. John J Hatch
   7. Jeffrey D Macklis

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important contribution to the field demonstrates the role of a single transcription factor with cell-autonomous functions in the differentiation of two distinct neuronal populations in regulating the interactions between those cells in a non-autonomous manner to generate their final organized projection pattern. There are additional quantifications and controls that would enhance the study and would improve the strength of the evidence from incomplete if they were performed.

   Reviewed by eLife

4. Fast and slow synaptic plasticity enables concurrent control and learning

   This article has 2 authors:

   1. Brendan A Bicknell
   2. Peter E Latham

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This paper provides an important proposal for why learning can be much faster and more accurate if synapses have a fast component that immediately corrects errors, as well as a slower component that corrects behavior averaged over a longer timescale. It is convincingly shown that integrating these two learning timescales improves performance compared to classical strategies, particularly in terms of robustness and generalization when learning new target signals. However, the biological plausibility and justification for the proposed rapid learning mechanism require further elaboration and supporting mechanistic examples.

   Reviewed by eLife

5. The myeloid cell-driven transdifferentiation of endothelial cells into pericytes promotes the restoration of BBB function and brain self-repair after stroke

   This article has 17 authors:

   1. Tingbo Li
   2. Ling Yang
   3. Jiaqi Tu
   4. Yufan Hao
   5. Zhu Zhu
   6. Yingjie Xiong
   7. Qingzhu Gao
   8. Lili Zhou
   9. Guanglei Xie
   10. Dongdong Zhang
   11. Xuzhao Li
   12. Yuxiao Jin
   13. Yiyi Zhang
   14. Bingrui Zhao
   15. Nan Li
   16. Xi Wang
   17. Jie-Min Jia

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study aims to understand the role of endothelial cell differentiation into pericytes in the restoration of blood-brain barrier function after ischemic stroke. Identification of pericytes derived from endothelial cells and the involvement of myeloid cell-derived TGFβ1 signaling are compelling new findings, but future studies will be needed to validate the origin and nature of these pericytes. The work will be of interest to blood-brain barrier and basic and translational stroke researchers.

   Reviewed by eLife

6. Optogenetic control of Protein Kinase C-epsilon activity reveals its intrinsic signaling properties with spatiotemporal resolution

   This article has 17 authors:

   1. Qunxiang Ong
   2. Crystal Jing Yi Lim
   3. Yilie Liao
   4. Justin Tze-Yang Ng
   5. Ler Ting Rachel Lim
   6. Shernys Xuan Yi Koh
   7. Sher En Chan
   8. Pheobe Lee Yu Ying
   9. Huijun Lim
   10. Chen Rui Ye
   11. Loo Chien Wang
   12. Siok Ghee Ler
   13. Radoslaw M Sobota
   14. Yaw Sing Tan
   15. Gerald I Shulman
   16. Xiaoyong Yang
   17. Weiping Han

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This manuscript introduces Opto-PKCε, an optogenetic tool that enabled important findings derived from interactome and phosphoproteome studies. Light-dependent recruitment of Opto-PKCε to the plasma membrane revealed the specific phosphorylation of the insulin receptor at Thr 1160. In turn, recruitment to mitochondria led to phosphorylation of the complex I subunit NDUFS4, correlating with reduced spare respiratory capacity. The evidence supporting these conclusions is solid, although additional clarification on data analysis would further enhance readability.

   Reviewed by eLife

7. A library of lineage-specific driver lines connects developing neuronal circuits to behavior in the Drosophila ventral nerve cord

   This article has 12 authors:

   1. Jelly HM Soffers
   2. Erin Beck
   3. Daniel J Sytkowski
   4. Marianne E Maughan
   5. Devasri Devarakonda
   6. Yi Zhu
   7. Beth A Wilson
   8. Yu-Chieh David Chen
   9. Ted Erclik
   10. James W Truman
   11. James B Skeath
   12. Haluk Lacin

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This work presents an important genetic toolkit for Drosophila neurobiologists to access and manipulate neuronal lineages during development and adulthood. The evidence supporting the fidelity of this toolkit after revision is compelling. This work will interest Drosophila neurobiologists in general, and some of the genetic tools may be used outside the nervous system. The conceptual approaches used in this paper are likely transferable to other fields as comparable data and genomic methods are obtained.

   Reviewed by eLife

8. The general version of Hamilton’s rule

   This article has 1 author:

   1. Matthijs van Veelen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     Kin selection and inclusive fitness have generated significant controversy. This paper reconsiders the general form of Hamilton's rule in which benefits and costs are defined as regression coefficients, with higher-order coefficients being added to accommodate non-linear interactions. The paper is a landmark contribution to the field with compelling, systematic analysis, giving clarity to long-standing debates.

   Reviewed by eLife

9. ImPaqT - A Golden Gate-based Toolkit for Zebrafish Transgenesis

   This article has 3 authors:

   1. Saskia Hurst
   2. Christiane Dimmler
   3. Mark R Cronan

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study introduces a valuable toolkit for zebrafish transgenesis, significantly enhancing the flexibility and efficiency of transgene generation for immunological applications. The authors provide convincing evidence through well-designed experiments, demonstrating the toolkit's utility in generating diverse and functional transgenic lines.

   Reviewed by eLife

10. Revealing global stoichiometry conservation architecture in cells from Raman spectral patterns

    This article has 6 authors:

    1. Ken-ichiro F Kamei
    2. Koseki J Kobayashi-Kirschvink
    3. Takashi Nozoe
    4. Hidenori Nakaoka
    5. Miki Umetani
    6. Yuichi Wakamoto

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This paper reports the fundamental finding of how Raman spectral patterns correlate with proteome profiles using Raman spectra of E. coli cells from different physiological conditions and found global stoichiometric regulation on proteomes. The authors' findings provide compelling evidence that stoichiometric regulation of proteomes is general through analysis of both bacterial and human cells. In the future, similar methodology can be applied on various tissue types and microbial species for studying proteome composition with Raman spectral patterns.

    Reviewed by eLife, Arcadia Science