eLife

Latest preprint reviews

1. Single-cell type analysis of wing premotor circuits in the ventral nerve cord of Drosophila melanogaster

   This article has 18 authors:

   1. Erica Ehrhardt
   2. Samuel C Whitehead
   3. Shigehiro Namiki
   4. Ryo Minegishi
   5. Igor Siwanowicz
   6. Kai Feng
   7. Hideo Otsuna
   8. FlyLight Project Team
   9. Geoffrey W Meissner
   10. David Stern
   11. James W Truman
   12. David Shepherd
   13. Michael H Dickinson
   14. Kei Ito
   15. Barry J Dickson
   16. Itai Cohen
   17. Gwyneth M Card
   18. Wyatt Korff

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important resource paper presents a library of cell-type-specific genetic driver lines that label wing-related motor and premotor neurons in the ventral nerve cord of the fruit fly, Drosophila. The toolkit is systematically validated with compelling anatomical and behavioral evidence and will provide a resource for future studies of Drosophila flight and courtship.

   Reviewed by eLife

2. Targeted and random mutagenesis of cassava brown streak disease susceptibility factors reveal molecular determinants of disease severity

   This article has 13 authors:

   1. ZJ Daniel Lin
   2. Myia K Stanton
   3. Gabriela L Hernandez
   4. Elizabeth J De Meyer
   5. Zachary von Behren
   6. Katherine Benza
   7. Helene Tiley
   8. Anna Meirink
   9. Emerald Hood
   10. Greg Jensen
   11. Kerrigan B Gilbert
   12. James C Carrington
   13. Rebecca S Bart

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This valuable study shows that targeted mutations in specific cassava eIF4E-family genes can reduce infection and disease symptoms caused by cassava brown streak viruses. Through systematic knockouts across the eIF4E gene family, the authors provide convincing evidence that certain double mutants show resistance-associated outcomes. Overall, the work supports practical routes to engineer cassava with improved resistance and clarifies which host factors are relevant for this disease.

   Reviewed by eLife

3. Esr1-Dependent Signaling and Transcriptional Maturation in the Medial Preoptic Area of the Hypothalamus Shapes the Development of Mating Behavior during Adolescence

   This article has 19 authors:

   1. Koichi Hashikawa
   2. Yoshiko Hashikawa
   3. Brandy Briones
   4. Kentaro Ishii
   5. Yuejia Liu
   6. Mark A Rossi
   7. Marcus L Basiri
   8. Jane Y Chen
   9. Omar R Ahmad
   10. Rishi V Mukundan
   11. Nathan L Johnston
   12. Rhiana C Simon
   13. James C Soetedjo
   14. Jason R Siputro
   15. Jenna A McHenry
   16. Richard D Palmiter
   17. David R Rubinow
   18. Larry S Zweifel
   19. Garret D Stuber

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The authors test the hypothesis that gonadal steroid signaling influences the transcriptional development of specific neurons in the mPOA during adolescence, and that such adolescent development of the mPOA is necessary for mating behaviors. The valuable findings are supported by convincing evidence. This work contributes new insight into hormone-sensitive transcriptional profiles within genetically defined neuron clusters in the mPOA during adolescence and will be of interest to systems and molecular neuroscientists and those interested in development, sex differences, and/or hormonal regulation.

   Reviewed by eLife

4. Defined cellular reprogramming of androgen receptor-active prostate cancer to neuroendocrine prostate cancer

   This article has 14 authors:

   1. Shan Li
   2. Kai Song
   3. Huiyun Sun
   4. Yong Tao
   5. Arthur Huang
   6. Vipul Bhatia
   7. Brian Hanratty
   8. Radhika A Patel
   9. Henry W Long
   10. Colm Morrissey
   11. Michael C Haffner
   12. Peter S Nelson
   13. Thomas G Graeber
   14. John K Lee

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The study by Li et al. provides fundamental findings supported by convincing evidence that they defined cellular reprogramming of androgen receptor in neuroendocrine prostate cancer (NEPC). The findings enhance the understanding of the treatment of androgen receptor functions in NEPC.

   Reviewed by eLife

5. Multiplexed CRISPRi Reveals a Transcriptional Switch Between KLF Activators and Repressors in the Maturing Neocortex

   This article has 5 authors:

   1. Ryan W Kirk
   2. Liwei Sun
   3. Ruixuan Xiao
   4. Erin A Clark
   5. Sacha Nelson

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This is an important study utilizing innovative CRISPR based approaches demonstrating the role of the KLF family of transcription factors in the post natal maturation of cortical projection neurons. The strength of evidence overall is compelling, and the study is well executed. The screen data presented provides a number of interesting candidates for future analyses into the mechanism of action of KLF family members in neuronal maturation.

   Reviewed by eLife

6. cxcl18b-defined transitional state-specific nitric oxide drives injury-induced Müller glia cell-cycle re-entry in the zebrafish retina

   This article has 6 authors:

   1. Aojun Ye
   2. Shuguang Yu
   3. Meng Du
   4. Dongming Zhou
   5. Jie He
   6. Chang Chen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     Following retinal injury, zebrafish Müller glia reenter the cell cycle and generate replacement cells; this potentially valuable study proposes that injury induces a cxcl18b+ transitional state in Müller cells, which then express nitric oxide, inhibiting Notch signaling and allowing Müller glial cells to reenter the cell cycle. However, the evidence supporting the claims is incomplete, and the authors have made interpretations and conclusions that are not supported by the data. Questions of the temporal expression and function of cxcl18b, as well as the source of potential inflammatory cues before cxcl18b expression, remain unanswered and technical limitations and data inconsistencies raise concerns. Using larval animals complicates the analysis since the retina is still forming, and distinguishing between injury-induced regeneration and ongoing development is complex. With more rigorous testing of the signaling pathways proposed and a clear demonstration of their interdependence, the link between nitric oxide signaling and Notch activity, particularly, would interest those investigating retinal regeneration.

   Reviewed by eLife

7. Affinity-guided labeling reveals P2X7 nanoscale membrane redistribution during BV2 microglial activation

   This article has 10 authors:

   1. Benoit Arnould
   2. Adeline Martz
   3. Pauline Belzanne
   4. Francisco Andrés Peralta
   5. Federico Cevoli
   6. Volodya Hovhannisyan
   7. Yannick Goumon
   8. Eric Hosy
   9. Alexandre Specht
   10. Thomas Grutter

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     The authors employ an unbiased, affinity-guided reagent to label P2X7 receptor and use super-resolution imaging to monitor P2X7 redistribution in response to inflammatory signaling. The evidence is convincing and the study will be valuable to those studying the dynamics of receptor distribution and clustering.

   Reviewed by eLife

8. Tumor Cell Spatial Organization Directs EGFR/RAS/RAF Pathway Primary Therapy Resistance through YAP Signaling

   This article has 11 authors:

   1. Rachel Nakagawa
   2. Andrew Beardsley
   3. Sophia Durney
   4. Mary-Kate Hayward
   5. Vishvak Subramanyam
   6. Nathaniel P Meyer
   7. Harrison Wismer
   8. Hani Goodarzi
   9. Valerie M Weaver
   10. Daniel Van de Mark
   11. Andrei Goga

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This study identifies a role for YAP in regulating tumor cell growth and drug response with differential effects noted based upon growth conditions in monolayer vs spheroid culture. This work has the potential to define more biologically relevant cell culture model systems for drug resistance and define targetable pathways to overcome drug resistance. The findings described are important to the cancer biology field and the evidence supporting the key findings is convincing.

   Reviewed by eLife

9. Imaging of brain electric field networks with spatially resolved EEG

   This article has 6 authors:

   1. Lawrence R Frank
   2. Vitaly L Galinsky
   3. Olave Krigolson
   4. Susan Tapert
   5. Stephan Bickel
   6. Antigona Martinez

   Reviewed by eLife

10. Unsupervised Representation Learning of C. elegans Poses and Behavior Sequences From Microscope Video Recordings

    This article has 2 authors:

    1. Maurice Deserno
    2. Katarzyna Bozek

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This valuable study introduces a self-supervised machine learning method to classify C. elegans postures and behaviors directly from video data, offering an alternative to the skeleton-based approaches that rely on often error-prone tracking. This novel approach holds promise for advancing ethology research. That said, the strength of evidence is currently incomplete, as key aspects - including measuring head-tail orientation, increased behavioral interpretability, and quantitative comparisons to established methods - are underdeveloped and would benefit from further validation.

    Reviewed by Arcadia Science, eLife