Synthetic Biology

Modular, robust, and extendible multicellular circuit design in yeast

1. Alberto Carignano
2. Dai Hua Chen
3. Cannon Mallory
4. R Clay Wright
5. Georg Seelig
6. Eric Klavins

• Curated by eLife

  Evaluation Summary:

  In this manuscript, the authors address important problems in the field of synthetic biology about scalability, robustness, and modularity. They used multiple strains to build gene circuits and demonstrate the modular composition of strain circuits with an automated design strategy to achieve a target behavior from a large space of possible functional circuit architectures. The major claims of the manuscript are well supported by solid quantitative data and systematic mathematical modeling analysis, and the approaches used are thoughtful and rigorous. This paper is of interest to synthetic biologists within the field of designing community-level behaviors, such as distributed computing, in multicellular consortia.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Integrated autolysis, DNA hydrolysis and precipitation enables an improved bioprocess for Q-Griffithsin, a broad-spectrum antiviral and clinical-stage anti-COVID-19 candidate

1. John S. Decker
2. Romel Menacho-Melgar
3. Michael D. Lynch

Reviewed by ScreenIT

Production and secretion of functional full-length SARS-CoV-2 spike protein in Chlamydomonas reinhardtii

1. Anna Kiefer
2. Justus Niemeyer
3. Anna Probst
4. Gerhard Erkel
5. Michael Schroda

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Predictive profiling of SARS-CoV-2 variants by deep mutational learning

1. Joseph M. Taft
2. Cédric R. Weber
3. Beichen Gao
4. Roy A. Ehling
5. Jiami Han
6. Lester Frei
7. Sean W. Metcalfe
8. Alexander Yermanos
9. William Kelton
10. Sai T. Reddy

Reviewed by ScreenIT

Peptide–Antibody Fusions Engineered by Phage Display Exhibit an Ultrapotent and Broad Neutralization of SARS-CoV-2 Variants

1. Jonathan M. Labriola
2. Shane Miersch
3. Gang Chen
4. Chao Chen
5. Alevtina Pavlenco
6. Reza Saberianfar
7. Francesca Caccuri
8. Alberto Zani
9. Nitin Sharma
10. Annie Feng
11. Daisy W. Leung
12. Arnaldo Caruso
13. Giuseppe Novelli
14. Gaya K. Amarasinghe
15. Sachdev S. Sidhu

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Proof of concept continuous event logging in living cells

1. Andrey Shur
2. Richard M. Murray

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A humanized nanobody phage display library yields potent binders of SARS CoV-2 spike

1. Ying Fu
2. Juliana da Fonseca Rezende e Mello
3. Bryan D. Fleming
4. Alex Renn
5. Catherine Z. Chen
6. Xin Hu
7. Miao Xu
8. Kirill Gorshkov
9. Quinlin Hanson
10. Wei Zheng
11. Emily M. Lee
12. Lalith Perera
13. Robert Petrovich
14. Manisha Pradhan
15. Richard T. Eastman
16. Zina Itkin
17. Thomas B. Stanley
18. Allen Hsu
19. Venkata Dandey
20. Kedar Sharma
21. William Gillette
22. Troy Taylor
23. Nitya Ramakrishnan
24. Shelley Perkins
25. Dominic Esposito
26. Eunkeu Oh
27. Kimihiro Susumu
28. Mason Wolak
29. Marc Ferrer
30. Matthew D. Hall
31. Mario J. Borgnia
32. Anton Simeonov

Reviewed by ScreenIT

A high-throughput, automated, cell-free expression and screening platform for antibody discovery

1. Andrew C. Hunt
2. Bastian Vögeli
3. Weston K. Kightlinger
4. Danielle J. Yoesep
5. Antje Krüger
6. Michael C. Jewett

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A Cell-Free Assay for Rapid Screening of Inhibitors of hACE2-Receptor–SARS-CoV-2-Spike Binding

1. Nanami Kikuchi
2. Or Willinger
3. Naor Granik
4. Reut Gal
5. Noa Navon
6. Shanny Ackerman
7. Ella Samuel
8. Tomer Antman
9. Noa Katz
10. Sarah Goldberg
11. Roee Amit

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A Genetic Trap in Yeast for Inhibitors of SARS-CoV-2 Main Protease

1. Hanna Alalam
2. Sunniva Sigurdardóttir
3. Catarina Bourgard
4. Ievgeniia Tiukova
5. Ross D. King
6. Morten Grøtli
7. Per Sunnerhagen

Reviewed by ScreenIT