Integrated autolysis, DNA hydrolysis and precipitation enables an improved bioprocess for Q-Griffithsin, a broad-spectrum antiviral and clinical-stage anti-COVID-19 candidate
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SciScore for 10.1101/2021.12.30.474602: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Antibodies Sentences Resources Plates were then blocked with 5% w/v BSA and incubated with clarified cell lysates containing 200 ng/mL total protein followed by incubation with a rabbit polyclonal anti-GRFT antibody (gift of Barry O’Keefe, National Cancer Institute, Bethesda, MD) at 1:400 dilution. anti-GRFTsuggested: NoneBound Q-GRFT was detected using an HRP-conjugated goat anti-rabbit polyclonal antibody at 1:5000 dilution and TMB (Cat. #65-6120 and #N301, respectively, Thermo Fisher Scientific, Waltham, MA) anti-rabbitsuggested: (Innovative Research …SciScore for 10.1101/2021.12.30.474602: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Antibodies Sentences Resources Plates were then blocked with 5% w/v BSA and incubated with clarified cell lysates containing 200 ng/mL total protein followed by incubation with a rabbit polyclonal anti-GRFT antibody (gift of Barry O’Keefe, National Cancer Institute, Bethesda, MD) at 1:400 dilution. anti-GRFTsuggested: NoneBound Q-GRFT was detected using an HRP-conjugated goat anti-rabbit polyclonal antibody at 1:5000 dilution and TMB (Cat. #65-6120 and #N301, respectively, Thermo Fisher Scientific, Waltham, MA) anti-rabbitsuggested: (Innovative Research Cat# 65-6120, RRID:AB_88384)Recombinant DNA Sentences Resources Strains and plasmids: E. coli strains DLF_R003, DLF_R004 [20], and DLF_Z0025 [21], as well as plasmid pHCKan-yibD-QGRFT plasmid (Addgene #158748) [18], were prepared as previously described. pHCKan-yibD-QGRFTsuggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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