Synthetic Biology

High-throughput development and characterization of new functional nanobodies for gene regulation and epigenetic control in human cells

1. Jun Wan
2. Abby R Thurm
3. Sage J Allen
4. Connor H Ludwig
5. Aayan N Patel
6. Lacramioara Bintu

• Curated by eLife

  eLife Assessment

  This manuscript describes a valuable screening approach to identifying nanobodies with the potential to modulate gene expression via epigenetic regulators. While the concept is of interest and the screening strategy is well designed, the current evidence supporting mechanistic specificity remains incomplete.

Reviewed by eLife

STAMPS: Signal-peptide Transformer for Augmenting Mammalian Protein Secretion

1. Jacopo Gabrielli
2. Marino Exposito-Rodriguez
3. Justina Briliūtė
4. Matthew Burrell
5. George Thom
6. Cleo Kontoravdi
7. Francesca Ceroni

Reviewed by Arcadia Science

An in vitro human vessel model to study Neisseria meningitidis colonization and vascular damages

1. Léa Pinon
2. Mélanie Chabaud
3. Pierre Nivoit
4. Jérôme Wong-Ng
5. Tri Tho Nguyen
6. Vanessa Paul
7. Charlotte Bouquerel
8. Sylvie Goussard
9. Pauline Smilovici
10. Emmanuel Frachon
11. Dorian Obino
12. Samy Gobaa
13. Guillaume Duménil

• Curated by eLife

  eLife Assessment

  The authors develop an important microfluidic microvascular model called "Vessel-on-Chip", which they use to study Neisseria meningitidis interactions within this in vitro vascular system. Compelling evidence shows that the fabricated channels are lined by endothelial cells, and these can be colonized by N. meningitidis that in turn triggers neutrophil recruitment. This model has advantages over the human skin xenograft mouse model, which requires complex surgical techniques, however, it also carries limitations in that only endothelial cells and supplied specific immune cells in the microfluidics are present, while true vasculature contains a number of other cell types including smooth muscle cells, pericytes, and components of the immune system.

  [Editors' note: this paper was reviewed by Review Commons.]

Reviewed by eLife, Review Commons

Synthetic gene circuits that selectively target RAS-driven cancers

1. Gabriel Senn
2. Leon Nissen
3. Yaakov Benenson

• Curated by eLife

  eLife Assessment

  This important study demonstrates the potential of synthetic gene circuits to detect and target aberrant RAS activity in cancer cell lines. The circuit design is novel and the evidence supporting the claims is convincing. As a proof-of-concept, this will be of broad interest to researchers in synthetic biology and therapeutics development, while future work will be required to help translate this technology toward clinical applications in cancer therapeutics and address potential limitations of the strategy.

Reviewed by eLife

Generative design of novel bacteriophages with genome language models

1. Samuel H. King
2. Claudia L. Driscoll
3. David B. Li
4. Daniel Guo
5. Aditi T. Merchant
6. Garyk Brixi
7. Max E. Wilkinson
8. Brian L. Hie

Reviewed by PREreview

Sustainable production of plastic-degrading enzymes in Chlamydomonas pacifica

1. Crisandra Jade Diaz
2. João Vitor Dutra Molino
3. Barbara Saucedo
4. Kalisa Kang
5. Évellin do Espirito Santo
6. Marissa Tessman
7. Abhishek Gupta
8. Michael D. Burkart
9. Ryan Simkovsky
10. Stephen Mayfield

Reviewed by Arcadia Science

CF2H: a Cell-Free Two-Hybrid platform for rapid protein binder screening

1. Julien Capin
2. Pauline Mayonove
3. Angelique DeVisch
4. Amon Becher
5. Giang Ngo
6. Alexis P Courbet
7. Robert J Ragotte
8. Martin Cohen-Gonsaud
9. Julien Espeut
10. Jerome Bonnet

Reviewed by Arcadia Science

The nanos ^d integral gene drive enables population modification of the malaria vector Anopheles gambiae

1. Pei-Shi Yen
2. Sebald ANR Verkuijl
3. Paolo Capriotti
4. Giuseppe Del Corsano
5. Astrid Hoermann
6. Maria Grazia Inghilterra
7. Irati Aramburu-Gonzalez
8. Moeez A Khan
9. Dina Vlachou
10. George K Christophides
11. Nikolai Windbichler

Reviewed by Review Commons

APEX: Automated Protein EXpression in Escherichia coli

1. Martyna Kasprzyk
2. Michael A. Herrera
3. Giovanni Stracquadanio

Reviewed by Arcadia Science

OT-Mation: an open-source code for parsing CSV files into python scripts for control of OT-2 liquid handling robotics

1. Alex Laverick
2. Katherine Convey
3. Catherine Harrison
4. Jenny Tomlinson
5. Jem Stach
6. Thomas P. Howard

Reviewed by Arcadia Science