1. Design of highly functional genome editors by modeling the universe of CRISPR-Cas sequences

    This article has 13 authors:
    1. Jeffrey A. Ruffolo
    2. Stephen Nayfach
    3. Joseph Gallagher
    4. Aadyot Bhatnagar
    5. Joel Beazer
    6. Riffat Hussain
    7. Jordan Russ
    8. Jennifer Yip
    9. Emily Hill
    10. Martin Pacesa
    11. Alexander J. Meeske
    12. Peter Cameron
    13. Ali Madani

    Reviewed by Arcadia Science

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  2. Intracellular Expression of a Fluorogenic DNA Aptamer Using Retron Eco2

    This article has 6 authors:
    1. Mahesh A Vibhute
    2. Corbin Machatzke
    3. Katrin Bigler
    4. Saskia Krümpel
    5. Daniel Summerer
    6. Hannes Mutschler
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important work introduces a method to express fluorogenic DNA aptamers in E. coli, paving the way for genetically encoded fluorescent DNA. The evidence supporting the conclusions is solid, consisting of comparisons of the aptamer's activity in vitro and within bacterial cells. This advancement described in this study is likely to become a standard technique in the DNA aptamer field, and the work will be of interest and utility to researchers in synthetic biology, molecular imaging, and bacterial genetics fields.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 1 listLatest version Latest activity
  3. A synthetic method to assay polycystin channel biophysics

    This article has 4 authors:
    1. Megan Larmore
    2. Orhi Esarte Palomero
    3. Neha P Kamat
    4. Paul G DeCaen
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The authors have developed a valuable approach that employs cell-free expression to reconstitute ion channels into giant unilamellar vesicles for biophysical characterisation. The work is solid and will be of particular interest to those studying ion channels that primarily occur in organelles and are therefore not amenable to be studied by more traditional methods.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 1 listLatest version Latest activity
  4. Targeted protein degradation systems to enhance Wnt signaling

    This article has 13 authors:
    1. Parthasarathy Sampathkumar
    2. Heekyung Jung
    3. Hui Chen
    4. Zhengjian Zhang
    5. Nicholas Suen
    6. Yiran Yang
    7. Zhong Huang
    8. Tom Lopez
    9. Robert Benisch
    10. Sung-Jin Lee
    11. Jay Ye
    12. Wen-Chen Yeh
    13. Yang Li
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The manuscript describes a valuable method to boost WNT signaling in a tissue-specific manner. The work extends previous data from the authors based on fusing an RSPO2 mutant protein to an antibody that binds ASGR1/2. In the current manuscript, two new antibodies with similar effects are described, that expand this solid approach and provide alternatives for potential future clinical applications. This manuscript will be of interest to all scientists studying protein engineering and cellular targeting.

    Reviewed by eLife

    This article has 6 evaluationsAppears in 1 listLatest version Latest activity
  5. Highly multiplexed design of an allosteric transcription factor to sense novel ligands

    This article has 13 authors:
    1. Kyle K. Nishikawa
    2. Jackie Chen
    3. Justin F. Acheson
    4. Svetlana V. Harbaugh
    5. Phil Huss
    6. Max Frenkel
    7. Nathan Novy
    8. Hailey R. Sieren
    9. Ella C. Lodewyk
    10. Daniel H. Lee
    11. Jorge L. Chávez
    12. Brian G. Fox
    13. Srivatsan Raman

    Reviewed by PREreview

    This article has 1 evaluationAppears in 1 listLatest version Latest activity
  6. Specific Modulation of CRISPR Transcriptional Activators through RNA-Sensing Guide RNAs in Mammalian Cells and Zebrafish Embryos

    This article has 5 authors:
    1. Oana Pelea
    2. Sarah Mayes
    3. Quentin RV. Ferry
    4. Tudor A. Fulga
    5. Tatjana Sauka-Spengler
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The authors aim to develop a CRISPR system that can be activated upon sensing an RNA. As an initial step to this goal, they describe RNA-sensing guide RNAs for controlled activation of CRISPR modification. Many of the data look convincing and while several steps remain to achieve the stated goal in an in vivo setting and for robust activation by endogenous RNAs, the current work will be important for many in the field.

    Reviewed by eLife

    This article has 7 evaluationsAppears in 1 listLatest version Latest activity
  7. Synthetic bacteria with programmed cell targeting and protein injection suppress tumor growth in vivo

    This article has 10 authors:
    1. Alejandro Asensio-Calavia
    2. Carmen Mañas
    3. Alba Cabrera-Fisac
    4. Eva Pico-Sánchez
    5. Elena M. Seco
    6. Starsha Kolodziej
    7. Daniel S. Leventhal
    8. José M. Lora
    9. Beatriz Álvarez
    10. Luis Ángel Fernández

    Reviewed by Arcadia Science

    This article has 3 evaluationsAppears in 1 listLatest version Latest activity
  8. Light-driven synchronization of optogenetic clocks

    This article has 5 authors:
    1. Maria Cristina Cannarsa
    2. Filippo Liguori
    3. Nicola Pellicciotta
    4. Giacomo Frangipane
    5. Roberto Di Leonardo
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study presents a light-entrainable synthetic oscillator in bacteria, the optorepressilator. The authors develop a toolbox using optogenetics that makes the cellular oscillator easily controllable. This toolbox is valuable, contributing both to bioengineering and to the understanding of biological dynamical systems. The comparison with a mathematical model, population, and single-cell measurements demonstrate convincingly that the planned system was achieved and is suitable to control and study biological oscillators.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 1 listLatest version Latest activity
  9. De novo-designed minibinders expand the synthetic biology sensing repertoire

    This article has 6 authors:
    1. Zara Y. Weinberg
    2. Sarah S. Soliman
    3. Matthew S. Kim
    4. Irene P. Chen
    5. Melanie Ott
    6. Hana El-Samad
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study presents a useful investigation to test de novo-designed mini binders against the Spike protein of SARS-CoV-2 within two classes of synthetic receptors (SNIPRs and CARs). The methods and evidence supporting the focused claims are very solid, although the small-scale nature of the investigation (number of modifications, number of minibinders, etc.) makes it difficult to determine how generalizable these results and potential design principles are. This work will be of interest to synthetic biologists and cell engineers as a starting point for systematic, larger-scale analysis and optimization of synthetic receptor designs for cellular therapy and other applications.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  10. A conserved phenylalanine motif among Teleost fish provides insight for improving electromagnetic perception

    This article has 5 authors:
    1. Brianna Ricker
    2. E. Alejandro Castellanos Franco
    3. Gustavo de los Campos
    4. Galit Pelled
    5. Assaf A. Gilad

    Reviewed by Arcadia Science

    This article has 5 evaluationsAppears in 1 listLatest version Latest activity