Plant Biology

Fast and Efficient Root Phenotyping via Pose Estimation

1. Elizabeth M. Berrigan
2. Lin Wang
3. Hannah Carrillo
4. Kimberly Echegoyen
5. Mikayla Kappes
6. Jorge Torres
7. Angel Ai-Perreira
8. Erica McCoy
9. Emily Shane
10. Charles D. Copeland
11. Lauren Ragel
12. Charidimos Georgousakis
13. Sanghwa Lee
14. Dawn Reynolds
15. Avery Talgo
16. Juan Gonzalez
17. Ling Zhang
18. Ashish B. Rajurkar
19. Michel Ruiz
20. Erin Daniels
21. Liezl Maree
22. Shree Pariyar
23. Wolfgang Busch
24. Talmo D. Pereira

Reviewed by PREreview

Soybean RIN4 represents a mechanistic link between plant immune and symbiotic signaling

1. Katalin Tóth
2. Daewon Kim
3. Sung-Hwan Cho
4. Cuong T. Nguyen
5. Tran H. N. Nguyen
6. Christopher Hartanto
7. Jean-Michel Michno
8. Adrian O. Stec
9. Robert M. Stupar
10. Gary Stacey

• Curated by eLife

  eLife assessment

  The manuscript describes important findings supported by convincing data. The authors present persuasive genetic and biochemical evidence that supports the biological mechanism for optimal nodulation in soybean presented in this study. The results are of relevance to understanding the signaling pathway underpinning beneficial rhizobia symbiosis, while repressing the immune response. With the discussion part strengthened this paper would be of broad interest to plant biologists working on cell signaling and plant-microbe interactions.

Reviewed by eLife

A Ralstonia solanacearum type III effector alters the actin and microtubule cytoskeleton to promote bacterial virulence in plants

1. Rachel Hiles
2. Abigail Rogers
3. Namrata Jaiswal
4. Weiwei Zhang
5. Jules Butchacas
6. Marcus V. Merfa
7. Taylor Klass
8. Erica Kaser
9. Jonathan M. Jacobs
10. Christopher J. Staiger
11. Matthew Helm
12. Anjali S. Iyer-Pascuzzi

Reviewed by PREreview

Antisense transcription from a neighboring gene interferes with the expression of mNeonGreen as a functional in vivo fluorescent reporter in the chloroplast of Chlamydomonas reinhardtii

1. Axel Navarrete
2. Bernardo Pollak

Reviewed by Arcadia Science

Competence for transcellular infection in the root cortex involves a post-replicative, cell-cycle exit decision in Medicago truncatula

1. Morgane Batzenschlager
2. Beatrice Lace
3. Ning Zhang
4. Chao Su
5. Sabrina Egli
6. Pascal Krohn
7. Jule Salfeld
8. Franck Anicet Ditengou
9. Thomas Laux
10. Thomas Ott

• Curated by eLife

  eLife assessment

  This is a fundamental cell biological study of host responses during symbiotic microbial infection of plants. Compelling imaging-based approaches using genetically-encoded cell cycle markers show that in Medicago truncatula root cortex cells, early rhizobial infection events are associated with cell-cycle re-entry, but once the infection is established, host cells exit the cell cycle. The work will be of interest to a wide range of colleagues, from development and cell biology to plant-microbe interactions.

Reviewed by eLife

The wheat powdery mildew resistance gene Pm4 also confers resistance to wheat blast

1. Tom O’Hara
2. Andrew Steed
3. Rachel Goddard
4. Kumar Gaurav
5. Sanu Arora
6. Jesús Quiroz-Chávez
7. Ricardo Ramírez-González
8. Roshani Badgami
9. David Gilbert
10. Javier Sánchez-Martín
11. Luzie Wingen
12. Cong Feng
13. Mei Jiang
14. Shifeng Cheng
15. Susanne Dreisigacker
16. Beat Keller
17. Brande B.H. Wulff
18. Cristóbal Uauy
19. Paul Nicholson

Reviewed by PREreview

Thermoprotection by a cell membrane–localized metacaspase in a green alga

1. Yong Zou
2. Igor Sabljić
3. Natalia Horbach
4. Adrian N Dauphinee
5. Anna Åsman
6. Lucia Sancho Temino
7. Elena A Minina
8. Marcin Drag
9. Simon Stael
10. Marcin Poreba
11. Jerry Ståhlberg
12. Peter V Bozhkov

Reviewed by Arcadia Science

Characterization of tryptophan oxidation affecting D1 degradation by FtsH in the photosystem II quality control of chloroplasts

1. Yusuke Kato
2. Hiroshi Kuroda
3. Shin-Ichiro Ozawa
4. Keisuke Saito
5. Vivek Dogra
6. Martin Scholz
7. Guoxian Zhang
8. Catherine de Vitry
9. Hiroshi Ishikita
10. Chanhong Kim
11. Michael Hippler
12. Yuichiro Takahashi
13. Wataru Sakamoto

• Curated by eLife

  eLife assessment:

  This study adds a fundamental new perspective to a long-standing question: What controls the repair of photosystem II (PSII), a key process in maintaining and optimizing photosynthesis? The work supports a role for chemical modification in the recognition and subsequent degradation of a key protein subunit of PSII by a bacterial-type protease, suggesting that tryptophan oxidation of components of the photosynthetic apparatus after high light stress plays a critical role in initiating the PSII repair system. The evidence supporting the authors' conclusions is solid.

Reviewed by eLife

Genome assembly of the bearded iris Iris pallida Lam

1. Robert E. Bruccoleri
2. Edward J. Oakeley
3. Ann Marie E Faust
4. Marc Altorfer
5. Sophie Dessus-Babus
6. David Burckhardt
7. Mevion Oertli
8. Ulrike Naumann
9. Frank Petersen
10. Joanne Wong

• Curated by GigaByte

  **Editors Assessment: **

  Irises on top of being a popular and beautiful ornamental plant, have wider commercial interest due to the many interesting secondary metabolites present in their rhizomes that have value to the fragrance and pharmaceutical industries. Many of these have large and difficult to assemble genomes, and to fill that gap the Dalmatian Iris (Iris pallida Lam.) is sequenced here. Using PacBio long-read sequencing and bionano optical mapping to produce a giant 10Gbp assembly with a scaffold N50 of 14.34 Mbp. The authors didn’t manage to handle the haplotigs separately or to study the ploidy, but as all of the data is available for reuse others can explore these questions further. This reference genome should also allow researchers to study the biosynthesis of these secondary metabolites in much greater detail, opening new avenues of investigation for drug discovery and fragrance formulations.

  This evaluation refers to version 1 of the preprint

Reviewed by GigaByte

Cell-type-specific control of secondary cell wall formation by Musashi-type translational regulators in Arabidopsis

1. Alicia Kairouani
2. Dominique Pontier
3. Claire Picart
4. Fabien Mounet
5. Yves Martinez
6. Lucie Le-Bot
7. Mathieu Fanuel
8. Philippe Hammann
9. Lucid Belmudes
10. Remy Merret
11. Jacinthe Azevedo
12. Marie-Christine Carpentier
13. Dominique Gagliardi
14. Yohann Couté
15. Richard Sibout
16. Natacha Bies-Etheve
17. Thierry Lagrange

• Curated by eLife

  eLife assessment

  Secondary cell walls support vascular plants and conduct water throughout the plant body, and are crucial resources for lignocellulosic feedstocks. Here the authors present convincing genetic and biochemical evidence that secondary cell wall synthesis, known already to be under complex transcriptional control, is also controlled post-transcriptionally by MUSASHI-like RNA-binding proteins. These important results point to a new mechanism for control of secondary cell wall synthesis, which will be interesting to cell biologists and biochemists studying and attempting to manipulate plant biomass.

Reviewed by eLife